Veterinary Research Forum
Vet Res Forum

Fabrication of chitosan-based electrospun nanofiber scaffold: Amplification of biomechanical properties, structural stability, and seeded cell viability

Document Type : Original Article

Authors

Mohammad Mahdian-Dehkordi 1
Farshid Sarrafzadeh-Rezaei 1
Mazdak Razi 2
Mehdi Mahmoudian 3

1 Department of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎

3 Nanotechnology Research Center, Urmia University, Urmia, Iran‎

https://doi.org/10.30466/vrf.2020.123047.2893
Abstract
The cell scaffolds should structurally be manufactured similar to the target tissue's extracellular matrix. This property should be maintained until cell differentiation. For this purpose, in the current study, electrospun nanofiber (EN) of chitosan (Ch)/polyvinyl alcohol (PVA), as a tissue-friend scaffold, was fabricated by electrospinning in different formulations and borax was utilized as an innovative cross-linking agent to up-regulate the structural and biomechanical properties. The weight loss, water absorbability, structural stability, tensile strength and biocompatibility of borax-included and non-included ENs were compared. The finest morphology, weight loss, water absorbability, structural stability in an aqueous environment, tensile strength and cell viability were found in the borax-included EN containing Ch50.00%v/PVA50.00%v. Moreover, The ENs exhibited appropriate antibacterial properties against Gram-positive and Gram-negative bacteria. In conclusion, borax can be used to improve the mechanical and biocompatibility features of the Ch/PVA-based ENs. Furthermore, it could be suggested that borax-included Ch/PVA ENs can exhibit high appropriate biological properties, candidate them as an appropriate scaffold in the field of tissue engineering. However, in vivo trials are needed to clearly their side effects and advantages.

Keywords

‎Biomaterial
Borax
Cell seeding
Cross linking
Electrospinning
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Veterinary Research Forum
Volume 12, Issue 1
Winter 2021
Pages 25-32

  • Receive Date 14 March 2020
  • Revise Date 27 April 2020
  • Accept Date 01 June 2020

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