Veterinary Research Forum
Vet Res Forum

Synergistic effects of dual antimicrobial combinations of synthesized N-heterocycles or MgO nanoparticles with nisin against the growth of Aspergillus fumigatus: In vitro study

Document Type : Original Article

Authors

Hamid Beyzaei 1
Mojtaba Mirzaei 2
Narjes Hasan Fakhrabadi 3
Behzad Ghasemi 4

1 Department of Chemistry, Faculty of Science, University of Zabol, Zabol, Iran

2 Young Researchers and Elite Club, Mashhad Branch, Islamic Azad University, Mashhad, Iran

3 Department of Pathobiology, Faculty of Veterinary, University of Zabol, Zabol, Iran

4 Torbat-e Jam Faculty of Medical Sciences, Torbat-e Jam, Iran

https://doi.org/10.30466/vrf.2019.103449.2460
Abstract
Introduction of new inhibitory agents such as peptides, heterocyclic derivatives and nanoparticles (NPs) along with preventive proceedings are effective ways to deal with standard and drug-resistant strains of microorganisms. In this regard, inhibitory activities of some recently synthesized 4-thiazolylpyrazoles, imidazolidine- and tetrahydropyrimidine-2-thiones and magnesium oxide (MgO) NPs alone and in combination with nisin have been assessed against Aspergillus fumigatus. Antimicrobial susceptibility tests were done via broth microdilution, disk diffusion and streak plate methods according to the modified Clinical and Laboratory Standards Institute (CLSI) guidelines. Synergistic effects were also determined as fractional inhibitory concentration (FIC) and fractional fungicidal concentration (FFC) values. Inhibitory potentials of all heterocycles and NPs against A. fumigatus were proved based on inhibition zone diameter (IZD) values in the range of 7.72 - 16.85 mm, minimum inhibitory concentration (MIC) values in the range of 64.00 - 512 µg mL-1 and minimum fungicidal concentration (MFC) values in the range of 256 - 2048 µg mL-1. Tetrahydropyrimidine derivative 3f showed the best inhibitory properties. Inhibitory activity was not significant with nisin. While antifungal effects of major derivatives were improved by combination with it. The results indicated that the combined treatment of heterocycles used in the present study with nisin might be efficient for mold prevention and removal in foodstuffs or other products.

Keywords

Aspergillus fumigatus
MgO nanoparticle
Nisin
Synergistic antifungal effect
Thiazole
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Veterinary Research Forum
Volume 12, Issue 2
Spring 2021
Pages 241-246

  • Receive Date 06 February 2019
  • Revise Date 04 August 2019
  • Accept Date 30 October 2019

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