Veterinary Research Forum
Vet Res Forum

Viability of in vitro preserved sperm of Harris’s hawk (Parabuteo unicinctus)

Document Type : Original Article

Authors

Cuauhtémoc Cruz-Valencia 1
José Antonio Herrera-Barragán 2
Fernando Gual-Sill 2
Juán José Pérez-Rivero 2
Zulma América Soto-Guerrero 3
Fernanda Rodriguez-Hernández 2
Juán Gabriel Rivera-Martínez 1, 4

1 Department of Animal Reproduction, University Autonomous Metropolitan, Iztapalapa, Mexico

2 Department of Agricultural and Animal Production, University Autonomous Metropolitan, Xochimilco, Mexico

3 Secretary of Environment, Bird of Prey Rehabilitation Center, Aguascalientes, Mexico

4 In memory

https://doi.org/10.30466/vrf.2020.106719.2534
Abstract
Harris hawks (Parabuteo unicinctus) are the raptors with more anthropogenic use, which makes it necessary to improve their reproduction either natural or assisted for sustainable use even though they are not endangered. The aim of this study was to determine fresh and thawed P. unicinctus sperm capacitation and acrosome reaction parameters as indicators of sperm viability in ejaculates of birds with injuries. A total of 30 ejaculates were analyzed as fresh and thawed samples. Basic sperm evaluations as well as in vitro acrosomal reaction capability through the presence and distribution of Ca2+ and N-acetylglucosamine and sialic acid using chlortetracycline and agglutinin-fluorescein isothiocyanate Triticum vulgaris were performed,respectively. Tagged samples were observed using epifluorescence microscopy. The results indicated 30.00% decrease in sperm viability following freeze-thaw, while rate of capacitated sperms (10.00%) and the percentage of sperm with acrosomal reaction (40.00%) were increased. The results of the experiment demonstrated that the post-thaw viability of the obtained sperm may be an alternative for use in artificial insemination.

Keywords

Acrosome
Cryopreservation
Glucosamine
Membrane
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Veterinary Research Forum
Volume 12, Issue 2
Spring 2021
Pages 137-141

  • Receive Date 23 April 2019
  • Revise Date 24 December 2019
  • Accept Date 03 May 2020

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