Veterinary Research Forum
Vet Res Forum

Season influence on serum kisspeptin level and its association with ‎hormonal levels and semen kinematics of buffalo bulls (Bubalus bubalis)‎

Document Type : Original Article

Authors

Muhammad Khurram Shahzad 1
Imtiaz Rabbani 1
Syed Murtaza Hassan Andrabi 2
Hafsa Zaneb 3
Muhammad Shahbaz Yousaf 1
Khalid Abdul Majeed 1
Sajid Khan Tahir 1
Sohrab Ahmad 1
Habib Rehman 1

1 Department of Physiology, University of Veterinary and Animal Sciences, Lahore, Pakistan‎

2 Animal Sciences Institute, National Agricultural Research Center, Islamabad, Pakistan‎

3 Department of Anatomy and Histology, University of Veterinary and Animal Sciences, Lahore, Pakistan

https://doi.org/10.30466/vrf.2020.122142.2873
Abstract
Kisspeptin has an important role in the stimulation of hypothalamic-pituitary-gonadal axis in term of pubertal development, release of reproductive and metabolic hormones and ultimately affecting the fertility. The aim of the present study was to evaluate the serum kisspeptin level and its correlation with semen quality and selected hormones in buffalo bulls during the summer and spring seasons. Semen and blood samples from eight Nili-Ravi buffalo bulls (age: 9.21 ± 1.02 years) were collected. Semen was analysed using computer-assisted semen analysis. Serum concentrations of kisspeptin, gonadotropin releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, cortisol, triiodothyronine (T3), thyroxin (T4) and insulin like growth factor (IGF-1) were estimated using enzyme-linked immunosorbent assay kits. Kisspeptin was neither affected by seasons and nor correlated with semen parameters and hormones. Higher levels of GnRH, LH, cortisol, IGF-1, total motility (TM), average path velocity (VAP), straight-line velocity (VSL), curvilinear velocity (VCL) and linearity (LIN) were recorded in summer compared to spring. Correlations of GnRH versusIGF-1 and LH, LH versus IGF-1 and cortisol, FSH versus T4 and testosterone, testosterone versus T3 and T4 and T3 versus T4 were observed. The GnRH and IGF-1 were positively associated with TM, VAP, VSL, VCL and LIN. The LH was correlated with VSL, straightness and LIN. In conclusion, GnRH, LH, and IGF-1 correlations with semen parameters can be used to indicate semen quality. The buffalo bulls are well-adapted and can give quality semen in the summer season.

Keywords

Bubalus bubalis
‎ Computer-assisted semen analysis
‎ Fertility
‎ Kisspeptin
‎ Season ‎

  1.  

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Veterinary Research Forum
Volume 12, Issue 3
Summer 2021
Pages 283-289

  • Receive Date 25 February 2020
  • Revise Date 11 May 2020
  • Accept Date 06 July 2020

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