Veterinary Research Forum
Vet Res Forum

Isolation of infectious laryngotracheitis virus in broiler chicken in Iran: First report

Document Type : Clinical Report

Authors

Jamshid Razmyar 1
Sara Shokrpoor 2
Abbas Barin 3
Jamshid Gheshlagh 4
Peyman Nakhaee 5
Moein Khodayari 1
Seyed Mostafa Peighambari 1

1 Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Private Poultry Clinic and Diagnostic Laboratory, Hamedan, Iran

5 Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University, Shiraz, Iran

https://doi.org/10.30466/vrf.2020.124157.2911
Abstract
In February 2019, a severe respiratory distress with co-infection of infectious laryngotracheitis (ILT) and Newcastle disease accompanied with Salmonella Enteritidis occurred in a broiler flock in the western region of Iran. Clinical signs included paralysis, torticollis, nasal discharge, conjunctivitis, gasping and respiratory rale with high mortality. At necropsy, caseous diphtheritic membrane adherent to the larynx and trachea was observed. Microscopically, syncytial cells formation with dense eosinophilic intranuclear inclusion bodies were main histopathological findings in tracheal tissues. Conventional polymerase chain reaction (PCR) for ICP4 gene amplification as a definitive diagnosis was utilized for the detection of ILT virus nucleic acid in suspected tracheal samples inoculated on to the chorioallantioc membrane of 11-day-old specific pathogen free (SPF) chicken eggs. Tracheal tissues taken from these SPF birds were positive by nested ILT PCR. In conclusion, because of no vaccination policy against ILT in broilers, the most probable scenario is that virus-laden dust or other fomites can be vectors and virus persistence and disease outbreak can be a sequel of wild virus introduction to the farm.

Keywords

Broiler
Histopathology
Infectious laryngotracheitis
Polymerase chain reaction
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Veterinary Research Forum
Volume 12, Issue 2
Spring 2021
Pages 259-262

  • Receive Date 06 April 2020
  • Revise Date 25 August 2020
  • Accept Date 07 September 2020

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