Veterinary Research Forum
Vet Res Forum

Serine proteases activity in miracidia of Fasciola hepatica and effects of ‎chemical and herbal inhibitors

Document Type : Original Article

Authors

Leila Kianifard 1
Mohammad Yakhchali 2
Mehdi Imani 3

1 PhD Candidate, Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2019.99959.2387
Abstract
Fasciolosis is a zoonotic parasitic disease caused by the trematode Fasciola hepatica. The proteases are essential for the survival of parasites. The present study was aimed to determine serine proteases activities in miracidia of F. hepatica and evaluate the effects of pH and different inhibitors on the serine proteases activities. Adult F. hepatica helminths were removed from naturally infected livers of the slaughtered cattle and crushed. The eggs were incubated at 28.00 ˚C for 16 days. The released miracidia were homogenized and total proteolytic activity of the extract of miracidia at different pH values were evaluated. Serine proteases activities were determined using specific substrates. The inhibitory effects of chemical and herbal inhibitors on the enzymes were also assessed. The extract of miracidia hydrolyzed azocasein with optimum activity at pH 8.00. The optimum pH effect on serine proteases activities was found at alkaline pH. Phenylmethylsulfonyl fluoride and Bowman-Birk inhibitors inhibited and decreased the proteases activities in the miracidia extract. It was concluded that there were proteases activities in miracidia of F. hepatica which were inhibited by chemical and herbal inhibitors.

Keywords

Fasciola hepatica
‎ Miracidia
‎ Serine proteases
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Veterinary Research Forum
Volume 12, Issue 3
Summer 2021
Pages 291-295

  • Receive Date 19 December 2018
  • Revise Date 26 February 2019
  • Accept Date 21 May 2019

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