Veterinary Research Forum
Vet Res Forum

Potential fertilizer of spermatozoa in porcine epididymal tail post-orchiectomy

Document Type : Original Article

Authors

Mariela Adriana Ydiaquez-Miranda 1
José Antonio Herrera-Barragán 2
Miguel González-Lozano 3, 4
Alejandro Ávalos-Rodríguez 3

1 PhD in Biological and Health Sciences, Metropolitan Autonomous University, ‎Mexico City, Mexico

2 Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, ‎Mexico City, Mexico

3 Department of Agricultural and Animal Production, Metropolitan Autonomous University-Xochimilco, ‎Mexico City, Mexico

4 ‎‎Center for Teaching, Research and Extension in Swine Production (CEIEPP), Faculty of ‎Veterinary Medicine and Animal Production, National Autonomous University of Mexico, Jilotepec, Mexico

https://doi.org/10.30466/vrf.2020.108342.2570
Abstract
The aim of this study was to determine the potential fertilizing of spermatozoa from the epididymal tail in different periods of time post-orchiectomy (P-OQ). Therefore, the study was approached in two stages. In the first stage, the orchiectomy was performed in 30 adult pigs. The testicles were stored at 5.00 ˚C in physiological saline solution for 5, 24, 48, 72, 96 and 120 hr. The spermatozoa were obtained by backflushing the vas deferens. The spermogram and fluorometric study were performed for each sample to evaluate the exposure of phosphatidyl-serine (PS) and acrosome reaction (AR). The second stage included the fertilization test, 16 prepubertal sows were selected, after synchronizing the oestrous cycle and the post-cervical artificial insemination was performed with the refrigerated sperm samples from each P-OQ time. The percentage of live sperm remained without significant changes until 96 hr P-OQ. An increase in the percentage of spermatozoa that showed a PS exposure was observed. The premature AR was evident after 72 hr. Considering that the artificial insemination was performed ensuring a minimum number of live sperms, no significant differences were observed in the number of embryos and corpora lutea. The results indicated that pig sperm collected from the epididymal tail P-OQ and stored for 5 and up to 72 hr at 5.00 ˚C had viable characteristics and maintained their fertilization ability. However, there was an increase in the loss of phospholipid asymmetry of the plasma membrane as time increased (72 and 96 hr), therefore, sperm viability was decreased.

Keywords

Fertilization
Phospholipid asymmetry
Sperm
Swine
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Veterinary Research Forum
Volume 12, Issue 3
Summer 2021
Pages 267-272

  • Receive Date 22 May 2019
  • Revise Date 24 April 2020
  • Accept Date 09 June 2020

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