Veterinary Research Forum
Vet Res Forum

Effects of feed restriction and supplementary garlic oil on blood metabolites in ewes

Document Type : Original Article

Authors

Ehsan Anassori 1
Vahid Mohammadi 1
Vahid Najarnezhad 1
Ghader Jalilzadeh-Amin 1
Hamed Khalilvandi-Behroozyar 2

1 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran‎

2 Department of Animal Science, Faculty of Agriculture, Urmia University, Urmia, Iran‎

https://doi.org/10.30466/vrf.2020.116116.2765
Abstract
The effects of garlic oil (GO) on serum fatty acids, lipid profiles and energy-related blood metabolites in feed-restricted fat-tailed ewes were investigated. For this purpose, twenty-eight dry, non-pregnant Makuei ewes (about three years of age; 45.00 ± 2.20 kg of body weight) were assigned randomly to four experimental groups including group 1 receiving basal diet as total mixed ration (TMR) without GO supplementation (control group), group 2 receiving 100% basal diet supplemented with GO (10.00 mg kg-1 BW), group 3 receiving 70.00% basal diet without GO supplementation and group 4 receiving 70.00% basal diet supplemented with GO (10.00 mg kg-1 BW). The main experimental period started eight weeks after performing adaptation and dietary allocations. Feed restriction reduced serum glucose levels along with higher serum levels of non-esterified fatty acids, triacylglycerols, β-hydroxybutyrate, low-density lipoprotein cholesterol, total cholesterol and very low-density of lipoprotein. Following feed restriction, the serum palmitic and oleic acids concentrations were increased. Garlic oil supplementation had a desirable effect on feed-restricted animals through lowering serum BHB and palmitic and oleic acids concentration and increasing the high-density lipoprotein cholesterol levels in the serum. These findings demonstrated that GO had the potential to reduce body-fat mobilization, thereby lowering the risk factors for disorders associated with negative energy balance in underfed ewes in the periparturient period.

Keywords

Energy
‎ Fatty acid profile
‎ Feed restriction
‎ Negative energy balance
  1. Constable P, Hinchcliff KW, Done SH, et al. Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats. 11th Philadelphia, USA: WB Saunders 2016; 1722-1726.
  2. Tekippe JA, Tacoma R, Hristov AN, et al. Effect of essential oils on ruminal fermentation and lactation performance of dairy cows. J Dairy Sci 2013; 96(12): 7892-7903.
  3. Anassori E, Dalir-Naghadeh B, Pirmohammadi R, et al. Garlic: A potential alternative for monensin as a rumen modifier. Livest Sci 2011; 142(1-3): 276-287.
  4. Ahmed MG, El-Zarkouny SZ, El-Shazly KA, et al. Impact of essential oils blend on methane emission, rumen fermentation characteristics and nutrient digestibility in Barki sheep. J Agri Sci 2014; 6(7): 144-156.
  5. Chaves AV, Stanford K, Dugan MER, et al. Effects of cinnamaldehyde, garlic and juniper berry essential oils on rumen fermentation, blood metabolites, growth performance, and carcass characteristics of growing lambs. Livest Sci 2008; 117(2-3): 215-224.
  6. Anassori E, Dalir‐Naghadeh B, Pirmohammadi R, et al. Changes in blood profile in sheep receiving raw garlic, garlic oil or monensin. J Anim Physiol Anim Nutr 2015; 99(1): 114-122.
  7. Panthee A, Matsuno A, Al-Mamun M, et al. Effect of feeding garlic leaves on rumen fermentation, methane emission, plasma glucose kinetics, and nitrogen utilization in sheep. J Anim Sci technol 2017; 59(1): 14. doi: 10.1186/s40781-017-0139-3.
  8. Vahidinia A, Komaki H, Rahbani M, et al. Effects of dietary garlic supplements on serum lipid profiles, LDL oxidation and weight gain in Western diet-fed rats. Prog Nutr 2017; 19(Supplement 1): 19-26.
  9. Sun YE, Wang W, Qin J. Anti-hyperlipidemia of garlic by reducing the level of total cholesterol and low-density lipoprotein: A meta-analysis. Medicine (Baltimore) 2018; 97(18): e0255. doi: 10.1097/MD.00000000 00010255.
  10. Kamruzzaman M, Torita A, Sako Y, et al. Effects of feeding garlic stem and leaf silage on rates of plasma leucine turnover, whole body protein synthesis and degradation in sheep. Small Ruminant Res 2011; 99(1): 37-43.
  11. Nam H, Jung H, Kim Y, et al. Aged black garlic extract regulates lipid metabolism by inhibiting lipogenesis and promoting lipolysis in mature 3T3-L1 adipocytes. Food Sci Biotechnol 2017; 27(2): 575-579.
  12. Zhu Z, Hang S, Zhu H, et al. Effects of garlic oil on milk fatty acid profile and lipogenesis‐related gene expression in mammary gland of dairy goats. J Sci Food Agric 2013; 93(3): 560-567.
  13. McGlone J, Ford S, Mitloehner F et al. Guide for the care and use of agricultural animals in research and teaching. 3rd Champaign-Urbana, Illinois: Federation of Animal Science Societies 2010: 141-155.
  14. Clevenger JF. Apparatus for the determination of volatile oil. J Am Pharm Assoc (1912) 1928; 17(4), 345-349.
  15. NRC, National Research Council. Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids, Washington, USA: National Academy Press 2007: 246-255.
  16. Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem 1972; 18(6): 499-502.
  17. Folch J, Lees M, Sloane Stanley GH. simple method for the isolation and purification of total lipids from animal tissues. J boil Chem 1957; 226(1): 497-509.
  18. Ichihara K, Fukubayashi Y. Preparation of fatty acid methyl esters for gas-liquid chromatography. J Lipid Res 2010; 51(3): 635-640.
  19. Russel AJF, Doney JM, Reid RL. Energy requirements of the pregnant ewe. J Agric Sci 1967; 68: 359-363.
  20. Gross J, van Dorland HA, Bruckmaier RM, et al. Performance and metabolic profile of dairy cows during a lactational and deliberately induced negative energy balance with subsequent realimentation. J Dairy Sci 2011; 94(4): 1820-1830.
  21. Carlson DB, Litherland NB, Dann HM, et al. Metabolic effects of abomasal L-carnitine infusion and feed restriction in lactating Holstein cows. J Dairy Sci 2006; 89(12): 4819-4834.
  22. Navarre CB, Pugh DG, Diseases of the gastrointestinal system. Sheep and goat medicine. 2002; 69-105.
  23. Caldeira RM, Belo AT, Santos CC, et al. The effect of long-term feed restriction and over-nutrition on body condition score, blood metabolites and hormonal profiles in ewes. Small Ruminant Res 2007; 68(3): 242-255.
  24. Yeh YY, Liu L. Cholesterol-lowering effect of garlic extracts and organosulfur compounds: human and animal studies. J Nutr 2001; 131(3s): 989S-993S.
  25. Duehlmeier R, Fluegge I, Schwert B, et al. Metabolic adaptations to pregnancy and lactation in German Blackheaded Mutton and Finn sheep ewes with different susceptibilities to pregnancy toxaemia. Small Ruminant Res 2011; 96(2-3): 178-184.
  26. Zhang S, Albornoz RI, Aschenbach JR, et al. Short-term feed restriction impairs the absorptive function of the reticulo-rumen and total tract barrier function in beef cattle. J Anim Sci 2013; 91(4): 1685-1695.
  27. Mahmoodi M, Hosseini Zijoud SM, Hassanshahi GH, et al. The effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and sugar metabolism in hyperglycemic and/or hyperlipidemic individuals-Benefit of raw garlic consumption. Adv Biol Chem 2011; 1: 29-33.
  28. Johnson DD, Mitchell GE, Tucker RE, et al. Pancreatic amylase, plasma glucose, and insulin responses to propionate or monensin in sheep. J Dairy Sci 1986; 69(1): 52-57.
  29. Xie W, Zhao Y, Zhang Y. Traditional Chinese medicines in treatment of patients with type 2 diabetes mellitus. Evid Based Complement Alternat Med 2011; 2011: 726723. doi: 10.1155/2011/726723.
  30. Cullen SP, Monahan FJ, Callan JJ, et al. The effect of dietary garlic and rosemary on grower-finisher pig performance and sensory characteristics of pork. Irish J Agr Food Res 2005; 44(1): 57-67.
  31. Ingvartsen KL. Feeding-and management-related diseases in the transition cow: Physiological adaptations around calving and strategies to reduce feeding-related diseases. Anim Feed Sci Technol 2006; 126(3-4): 175-213.
  32. Drackley JK, Overton TR, Neil Douglas G. Adaptations of glucose and long-chain fatty acid metabolism in liver of dairy cows during the periparturient period. J Dairy Sci 2001; 84(Supplement): E100-E112.
  33. Bauchart D. Lipid absorption and transport in ruminants. J Dairy Sci 1993; 76(12): 3864-3881.
  34. Bartley JC. Lipid metabolism and its diseases. In: Kaneko JJ (Ed). Clinical biochemistry of domestic animals. 4th New York, USA: Academic Press 1989; 106-141.
  35. Gupta N, Porter TD. Garlic and garlic-derived compounds inhibit human squalene monooxygenase. J Nutr 2001; 131(6): 1662-1667.
  36. Mohammadi A, Abbasi Oshaghi E. Effect of garlic on lipid profile and expression of LXR alpha in intestine and liver of hypercholesterolemic mice. J Diabetes Metab Disord 2014; 13(1): 20. doi: 10.1186/2251-6581-13-20.
  37. Lin MC, Wang EJ, Lee C, et al. Garlic inhibits microsomal triglyceride transfer protein gene expression in human liver and intestinal cell lines and in rat intestine. J Nutr 2002; 132(6): 1165-1168.
  38. Tanaka Y, Mori A, Tazaki H, et al. Plasma metabolite concentrations and hepatic enzyme activities in pregnant Romney ewes with restricted feeding. Res Vet Sci 2008; 85(1): 17-21.
  39. Rowe A, Macedo FAF, Visentainer JV, et al. Muscle composition and fatty acid profile in lambs fattened in drylot or pasture. Meat Sci 1999; 51(4): 283-288.
  40. Neil Douglas G, Rehage J, Beaulieu AD, et al. Prepartum nutrition alters fatty acid composition in plasma, adipose tissue, and liver lipids of periparturient dairy cows. J Dairy Sci 2007; 90(6): 2941-2959.
  41. Kim YJ, Jin SK, Yang HS. Effect of dietary garlic bulb and husk on the physicochemical properties of chicken meat. Poult Sci 2009; 88(2): 398-405.
  42. Hwang YP, Kim HG, Choi JH, et al. S-allyl cysteine attenuates free fatty acid-induced lipogenesis in human HepG2 cells through activation of the AMP-activated protein kinase-dependent pathway. J Nutr Biochem 2013; 24(8): 1469-1478.
  43. Lee MS, Kim IH, Kim CT, et al. Reduction of body weight by dietary garlic is associated with an increase in uncoupling protein mRNA expression and activation of AMP-activated protein kinase in diet-induced obese mice. J Nutr 2011; 141(11): 1947-1953.
  44. Brindle NP, Zammit VA, Pogson CI. Regulation of carnitine palmitoyltransferase activity by malonyl-CoA in mitochondria from sheep liver, a tissue with a low capacity for fatty acid synthesis. Biochem J 1985; 232(1): 177-182.
  45. Reid JC, Husbands DR. Oxidative metabolism of long-chain fatty acids in mitochondria from sheep and rat liver. Evidence that sheep conserve linoleate by limiting its oxidation. Biochem J 1985; 225(1): 233-237.
  46. Reuter HD, Koch, HP, Lawson D. Therapeutic effects of garlic and its preparations. In: Lawson LD, Koch HP (Eds). Garlic: The science and therapeutic applications of Allium sativum and related species. 2nd ed. Baltimore, USA: William & Wilkins 1996; 135-162.
  47. He ML, Yang WZ, You JS, et al. Effect of garlic oil on fatty acid accumulation and glycerol-3-phosphate dehydrogenase activity in differentiating adipocytes. Asian-Aust J Anim Sci 2009; 22(12): 1686-1692.
Veterinary Research Forum
Volume 12, Issue 3
Summer 2021
Pages 369-376

  • Receive Date 22 October 2019
  • Revise Date 04 February 2020
  • Accept Date 19 April 2020

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