Veterinary Research Forum
Vet Res Forum

Application of metagenomic sequencing toward rapid and sensitive ‎diagnosis of goose avastrovirus infection in China

Document Type : Original Article

Authors

Guangwei Zhao 1
Xinzhu Deng 1
Di Wu 1
Ruibing Cao 2
Aojun Shao 3
Yu Zhou 3
Tianqi Zhang 3
Ge Li 3
Hongzhang He 3
Jing Lu 1
Liwu Zhang 3

1 Department of Veterinary Medicine, College of Veterinary Medicine, Southwest University, Chongqing China‎

2 Department of Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China‎

3 Chongqing Sanjiezhongxin Bioengineering Co., Ltd, Chongqing, China

https://doi.org/10.30466/vrf.2020.116892.2776
Abstract
The gosling gout, a newly emerged disease, has widely broken out in China since 2017. Typical signs for the disease include diarrhea, anorexia, depression, dehydration, emaciation and paralysis. At autopsy, uratosis was the main pathological change which could be found at kidney, pericardium, air sac, muscle and leg joint. In this study, gosling gout was firstly diagnosed by metagenomic analysis. Samples of kidney, Fabricius bursa, spleen and jejunum were collected and submitted to next-generation DNA sequencing. Our results demonstrated that goose avastrovirus was highly related with this disease. We confirmed the sequencing results by reverse transcription polymerase chain reaction method and artificial infection experiment and got consistent results. In summary, metagenomic sequencing method combined with traditional molecular identification was applied toward diagnosis of a novel gosling gout disease in China and revealed that goose avastrovirus was highly related with this disease. It has been proved to be a powerful tool for rapid and sensitive diagnosis of animal diseases, especially for some exceptional pathogens. In addition, host range, variation, molecular pathogenesis and potential zoonotic infection of this novel goose astrovirus need to be further studied.

Keywords

China
Diagnosis
Goose avastrovirus
Gosling gout
Metagenomic analysis
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Veterinary Research Forum
Volume 13, Issue 1
Winter 2022
Pages 1-6

  • Receive Date 07 November 2019
  • Revise Date 09 January 2020
  • Accept Date 12 February 2020

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