Veterinary Research Forum
Vet Res Forum

Pulsed-field gel electrophoresis fingerprinting of Listeria monocytogenes ‎isolates recovered from foods of animal origin and fishes in North-Eastern ‎India

Document Type : Short Communication

Authors

Ingudam Shakuntala 1
Arockiasamy Arun Prince Milton 1
Samir Das 1
Sandeep Ghatak 1
Rajkumari Sanjukta 1
Kekungu-U Puro 1
Raj Kumar Pegu 1
Swapnil Prakash Doijad 2
Sukhadeo Balirao Barbuddhe 3
Arnab Sen 1

1 Division of Animal Health, Indian Council of Agricultural Research (ICAR) Research Complex for North Eastern Hill Region, Umiam, India‎

2 Department of Microbiology, Goa University, Taleigaon Plateau, India

3 ICAR-National Research Centre on Meat, Hyderabad, India

https://doi.org/10.30466/vrf.2020.121715.2865
Abstract
Listeria monocytogenes is a pathogen of great concern to the food industry. The present study was aimed to explore the clonal relationships amongst L. monocytogenes strains isolated from foods of animal origin (milk, beef, chevon (goat meat), pork and chicken) and fish. Forty-six L. monocytogenes strains were characterized by pulsed-field gel electrophoresis (PFGE). The PFGE analysis using ApaI and AscI enzymes revealed 37 pulsotypes, with Simpson’s discriminatory index of 0.987. This study demonstrated the presence of a few similar L. monocytogenes pulsotypes in different foods of animal origin in different places and years of isolation and this indicates that some L. monocytogenes subtypes may be ubiquitous which are acclimatizing and persisting in different foods of animal origin. This also emphasizes the importance of cross-contamination in local wet markets. Thus, the understanding of genetic diversity will contribute to the development of rational and workable strategies to control this important zoonotic infection. 

Keywords

Food
‎ Genetic diversity
‎ India
‎ Listeria monocytogenes
‎ Pulsed-field gel electrophoresis ‎

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Veterinary Research Forum
Volume 13, Issue 1
Winter 2022
Pages 133-139

  • Receive Date 15 February 2020
  • Revise Date 30 June 2020
  • Accept Date 03 August 2020

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