Veterinary Research Forum
Vet Res Forum

Histological and stereological approaches for detection of tissues and fraud in some meat products ‎

Document Type : Original Article

Authors

Nasser Darban Maghami 1
Abolghasem Nabipour 1
Mohammad Mohsenzadeh 2
Maryam Torabi 3

1 Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran‎

2 Department of Food Hygiene, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran‎

3 Laboratory of Veterinary Office, Mashhad, Iran‎

https://doi.org/10.30466/vrf.2020.115238.2742
Abstract
Meat and meat products are highly important sources of protein in the diet. Nowadays, the consumption of meat and meat products has increased owing to modern manufacturing techniques. Due to the economic value of meat, the use of unauthorized tissue is possible in meat products. In some cases, there is fraud in the percentage of meat in meat products to reduce prices. In this study, 34 samples of minced meat, hamburger and sausage were randomly collected from the markets in the northeast of Iran. Then, sections were stained using Hematoxylin and Eosin (H & E), Verhoeff-van-Gieson, Masson's trichrome and periodic acid–Schiff-Alcian blue stains. In this regard, for the first time, the efficacy of stereological technique to determine the percentage of meat listed in sausages and the possible existence of fraud was evaluated. The results showed that, due to the presence of some unusual tissues, histological technique could determine different tissues in meat products. The stereological results of control samples showed a very slight difference; whereas, the results for the samples collected from the city stores showed a distinctive difference regarding the percentage of meat compared to the percentage of label. Skeletal and smooth muscles, blood vessels, nerve, gizzard, adipose tissue, glandular tissue, cartilage, bone, tendon, skin, lymphatic tissues and plant materials were observed. It was confirmed that stereology was a reliable method to determine and confirm the percentage of meat used in meat products.

Keywords

Authorized tissues
‎ Histology
‎ Meat product
‎ Stereology
‎ Unauthorized tissues

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    1. Mohammadi H. Principles of nutrition and dietary [Persian]. Tehran, Iran: University of Tehran Press 1999; 46-47.
    2. Majedi M. Food chemical test methods [Persian]. Tehran, Iran: Jahad Daneshgahi Press 1998; 91-93.
    3. Bergeron M, Durand P. The histological technique called "encogelation" application to the study of the probability of the identification of a constituent [French]. Ann Fals Exp Chim 1976; 69: 669-671.
    4. Kamkar AAF, Rokny N, Rasouli A, et al. Evaluating of hamburger quality using collagen content [Persian]. Pajouhesh-va-Sazandegi 2004; 17(2): 75-79.
    5. Potter NN, Hotchkiss JH. Food Science. 5th New York, USA: Springer 2012; 491-3, 67.
    6. de Macedo Neto JJ, dos Santos JA, Schwartz WR. Meat adulteration detection through digital image analysis of histological cuts using LBP. arXiv preprint arXiv:1611.02260, 2016. Accessed 20 Feb 2022.
    7. Standard Organization of Iran. Sausages specifications and test methods (Amendment No. 2) [Persian]. 2010; No. 2303.
    8. Kamkar A, Bokaei S, Behrozi M, et al. Determination of hydroxyproline as measure of collagen content in meat product by colorimetric method [Persian]. J Vet Res 2005; 60(1): 25-30.
    9. Abbasy-Fasarani M, Hosseini H, Jahed-Khaniki GR, et al. Histological study of industrial hamburgers containing 30 and 60 percent meat for presence of unpermitted edible tissues and correlation of this factor to meat connective tissue chemical indices [Persian]. Iran J Nutr Sci Food Technol 2013; 7(5): 311-318.
    10. Fekri M, Hosseini H, Eskandari S, et al. Histological study of sausages in point of unpermitted edible tissues assessment and its relationship to collagen and hydroxyproline of product [Persian]. J Food Sci Technol (Iran) 2013; 10(41): 107-116.
    11. Sadeghi E, Khazaei M, Almasi A, et al. Recognition of illegal tissues in the meat products from Kermanshah Supply Centers during the years 2009-2010. Horizon Med Sci 2011; 17(1): 55-59.
    12. Gundersen HJ. Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J Microsc 1986; 143(Pt 1): 3–
    13. Gundersen HJ, Bendtsen TF, Korbo L, et al. Some new, simple and efficient stereological methods and their use in pathological research and diagnosis. APMIS 1988; 96(5): 379-394.
    14. Gundersen HJ, Jensen EB. The efficiency of systematic sampling in stereology and its prediction. J Microsc 1987; 147(Pt 3): 229-263.
    15. Sahin B, Aslan H, Unal B. Brain volumes of the lamb, rat and bird do not show hemispheric asymmetry: a stereological study. Image Anal Stereol 2001; 20(1): 9-13.
    16. Hannan I. The collagen content of meat products and its legislative implications. J Sci Food Agric 1989; 35: 126-129.
    17. Ballin NZ. Authentication of meat and meat products. Meat Sci 2010; 86(3): 577-587.
    18. Rokni N, Rezaian M, Dayani Dardashti A. Histological histometrical study of different heated sausages [Persian]. J Vet Med 1997; 52: 95-103.
    19. Barai BK, Nayak RR, Singhal RS, et al. Approaches to detection of meat adulteration. Trends Food Sci Technol 1992; 3: 69-72.
    20. Carey AM, Archer NA, Priore JD Jr, et al. Histological detection of cardiac musculature, soy flour, and partially defatted tissue in ground beef: interlaboratory study. J Assoc Off Anal Chem 1984; 67(1): 16-19.
    21. Izadi F, Sadeghinejad J, Hajimohamadi B, et al. Efficacy of histological examination in detection of Fraud in minced meat [Persian]. J Health 2016; 7(4): 386-394.
    22. Sepehri Eraei S. Histological methods evaluation for detection of adulteration of raw meat products supplied in Tehran. DVM Thesis. University of Tehran. Tehran, Iran 2008.
    23. Prayson B, McMahon JT, Prayson RA. Fast food hamburgers: what are we really eating? Ann Diagn Pathol 2008; 12(6): 406-409.
    24. Darban Maghami N, Nabipour A, Mohsenzadeh M, et al. Detection of minced red meat mixing adulteration via molecular and histological techniques in Mashhad, Iran. Health Dev J 2021; 10(1): 64-73.
    25. Hassanzadeh Yazdi J. Histological study of heated sausages in Khorasan province. DVM Thesis. Ferdowsi University of Mashhad. Mashhad, Iran 2000.
    26. Jahed Khaniki GR, Rokni ND. Histological detection of soya in freezing raw hamburger of Iran [Persian]. Pajouhesh-va-Sazandegi 2004; 17(1): 71-75.
    27. Cetin O, Bingol EB, Civan E, et al. Identification of animal species and foreign tissues in ready-to-sell fresh processed meat products. Acta Aliment 2016; 45(2): 198-205.
    28. Latorre R, Sadeghinezhad J, Hajimohammadi B, et al. Application of morphological method for detection of unauthorized tissues in processed meat products. J Food Qual Hazards Control 2015; 15(2): 71-74.
    29. Prayson BE, McMahon JT, Prayson RA. Applying morphologic techniques to evaluate hotdogs: what is in the hotdogs we eat? Ann Diagn Pathol 2008; 12(2): 98-102.
Veterinary Research Forum
Volume 13, Issue 1
Winter 2022
Pages 47-53

  • Receive Date 02 October 2019
  • Revise Date 11 December 2019
  • Accept Date 08 January 2020

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