Document Type : Original Article
Institute for Advanced Studies, University of Malaya, Kuala Lumpur, Malaysia
Centre for Foundation Studies in Science, University of Malaya, Kuala Lumpur, Malaysia
Centre for Research in Biotechnology for Agriculture (CEBAR), University of Malaya, Kuala Lumpur, Malaysia
Histamine widely involves in local immune responses, physiological function in the gut, and acting as a neurotransmitter in the brain. Scientist also found the importance of histamine in the reproductive systems. The present study aimed to determine the existence of histamine receptor subtypes; H1R, H2R, H3R, and H4R on mouse oocytes through immunofluorescence (IF) staining and reverse transcription- polymerase chain reaction (RT-PCR). These further confirmed by the involvement of histamine receptor antagonists in in vitro fertilization (IVF). In IF staining, mouse oocytes were incubated with primary antibody against histamine receptor, followed by incubation with fluorescence conjugated secondary antibody. Then RT-PCR analysis was carried out for the undetected receptors during IF for confirmation. The RT-PCR used RNA extracted from mice COCs and cumulus free oocytes. In IVF, sperm was cultured in a group of treated histamine receptor antagonists oocytes. This investigation revealed the existance of H1R, H2R, and H3R on mouse oocytes in IF and RT-PCR analyses. The treatment of IVF with histamine receptor antagonists (H1R: pyrilamine; H2R: cimetidine; H3R: thioperamide) led to a significant reduction quantity of 2-cell embryos (4.61 ± 2.44%; 5.83 ± 4.65%; 3.83 ± 1.82%, respectively) as compared with the control group (22.50 ± 6.44%). Therefore, according to the results of this study, the presence of H1R, H2R, and H3R on mouse oocytes possibly will suggest the involvement of histamine in fertilization.
- Waldum HL, Sandvik AK, Syversen U, et al. The entero-chromaffin-like (ECL) cell. Physiological and patho-physiological role. Acta Oncol 1993; 32(2): 141-147.
- Chotard C, Ouimet T, Morisset S, et al. Effects of histamine H3 receptor agonist and antagonist on histamine co-transmitter expression in rat brain. J Neural Trans (Vienna) 2002; 109(3): 293-306.
- Hallgren J, Gurish MF. Granule maturation in mast cells: Histamine in control. Eur J Immunol 2014; 44(1): 33-36.
- Borriello F, Iannone R, Marone G. Histamine release from mast cells and basophils. Handb Exp Pharmacol 2017; 241: 121-139.
- Lagunoff D, Benditt EP. Mast cell degranulation and histamine release observed in a new In vitro system. J Exp Med 1960; 112(4): 571-580.
- Ishikawa T, Shimada T, Kessoku N, et al. Inhibition of rat mast cell degranulation and histamine release by histamine-rat gammaglobulin conjugate. Int Arch Allergy Appl Immunol 1979; 59(4): 403-407.
- Dy M, Schneider E. Histamine-cytokine connection in immunity and hematopoiesis. Cytokine Growth Factor Rev 2004; 15(5): 393-410.
- Akdis CA, Simons Histamine receptors are hot in immunopharmacology. Eur J Pharmacol 2006; 533
- Chazot PL, Tiligada E. The European Histamine Research Society (EHRS) symposium for EPHAR 2008 Inflamm Res 2008; 57(Suppl 1): S05- S06.
- Hill SJ. Distribution, properties, and functional characteristics of three classes of histamine receptor. Pharmacol Rev 1990; 42(1):45-83.
- Feuerstein TJ. Presynaptic receptors for dopamine, histamine, and serotonin. Handb Exp Pharmacol 2008; (184): 289-338.
- Aquino-Miranda G, Osorio-Espinoza A, Escamilla-Sánchez J, et al. Histamine H3 receptors modulate depolarization-evoked [3H]-noradrenaline release from rat olfactory bulb slices. Neuropharmacology 2012; 62(2): 1127-1133.
- Oda T, Morikawa N, Saito Y, et al. Molecular cloning and characterization of a novel type of histamine receptor preferentially expressed in leukocytes. J Biol Chem 2000; 275(47): 36781-36786.
- Lippert U, Artuc M, Grützkau A, et al. Human skin mast cells express H2 and H4, but not H3 receptors. J Inves Dermatol 2004; 123(1):116-123.
- Mayerhofer A, Bartke A, Amador AG. Histamine affects testicular steroid production in the golden hamster. Endocrinology 1989; 125(4): 2212-2214.
- Albrecht M, Frungieri MB, Gonzalez-Calvar S, et al. Evidence for a histaminergic system in the human testis. Fertil Steril 2005; 83(4): 1060-1063.
- Mondillo C, Patrignani Z, Reche C, et al. Dual role of histamine in modulation of Leydig cell steroidogenesis via HRH1 and HRH2 receptor subtypes. Biol Reprod 2005; 73(5): 899-907.
- Mondillo C, Falus A, Pignataro O, et al. Prolonged histamine deﬁciency in histidine decarboxylase gene knockout mice affect Leydig cell function. J Androl 2007; 28(1): 86-91.
- Mondillo C, Pagotto RM, Piotrkowski B, et al. Involvement of nitric oxide synthase in the mechanism of histamine-induced inhibition of Leydig cell steroid-genesis via histamine receptor subtypes in Sprague-Dawley rats. Biol Reprod 2009; 80(1): 144-152.
- Khan UW, Rai U. Differential effects of histamine on Leydig cell and testicular macrophage activities in wall lizards: precise role of H1/H2 receptor subtypes. J Endocrinol 2007; 194(2): 441-448.
- Pap E, Rácz K, Kovács JK, et al. Histidine decarboxylase deﬁciency in gene knockout mice elevates male sex steroid production. J Endocrinol 2002; 175(1): 193-199.
- Saﬁna F, Tanaka S, Inagaki M, et al. Expression of L-histidine decarboxylase in mouse male germ cells. J Biol Chem 2002; 277(16): 14211-14215.
- Temeles GL, Ram PT, Rothstein JL, et al. Expression patterns of novel genes during mouse preimplantation embryogenesis. Mol Reprod Dev 1994; 37(2): 121-129.
- Leguia M, Wessel GM. The histamine H1 receptor activates the nitric oxide pathway at fertilization. Mol Reprod Dev 2006; 73(12): 1550-1563.
- Whitaker M. Calcium at fertilization and in early development. Physiol Rev 2006; 86(1): 25-88.
- Berridge MJ, Lipp P, Bootman MD. The versatility and universality of calcium signalling. Nat Rev Mol Cell Biol 2000; 1(1): 11-21.
- Kotlikoff MI, Murray RK, Reynolds EE. Histamine-induced calcium release and phorbol antagonism in cultured airway smooth muscle cells. Am J Physiol 1987; 253(4 Pt 1): C561-C566.
- Wozniak KL, Mayfield BL, Duray AM, et al. Extracellular Ca2+ is required for fertilization in the African Clawed Frog, Xenopus laevis. PLoS One 2017; 12 (1): e0170405. doi: 10.1371/journal.pone.0170405.
- Sinha RB, Banerjee P, Ganguly AK. Serum concentration of testosterone, epididymal mast cell population and histamine content in relation to sperm count and their motility in albino rats following H2 receptor blocker treatment. Nepal Med Coll J 2006; 8(1): 36-39.
- Aprioku JS, Ibeachu C, Amah-Tariah FS. Differential effects of H2 receptor antagonists on male reproductive function and hepatic enzymes in Wistar rats. Asian J Biomed Pharm Sci 2014; 4(28): 1-6.
- Banihani SA. Histamine-2 receptor antagonists and semen quality. Basic Clin Pharmacol Toxicol 2016; 118(1): 9-13.
- Ahmad EA, Abdel-Emam RA. The potential impact of 1st and 2nd generation antihistamines on male fertility. Comp Clin Path 2019; 28(5): 1465-1470.
- Oraibi TS, Ibrahim RM, Soud SA. Effects of ranitidine (Zantac) drug on the hormonal level, sperm head abnormality and histoarchitecture of the testis of albino male mice. Asian J BiotechnolBioresou Technol 2019; 5(1): 1-7.
- Nemetallah BR, Howell RE, Ellis LC. Histamine H1receptors and prostaglandin histamine interactions modulating contractility of rabbit and rat testicular capsules in Vitro. Biol Reprod 1983; 28(3): 632-635.
- Abiuso AM, Berensztein E, Pagotto RM, et al. H4 histamine receptors inhibit steroidogenesis and proliferation in Leydig cells. J Endocrinol 2014; 223(3): 241-253.
- Virant-Klun I, Knez K, Tomazevic T, et al. Gene expression profiling of human oocytes developed and matured in vivo or in vitro. BioMed Res Int 2013: 879489. doi: 10.1155/2013/879489.
- Okabe M. The cell biology of mammalian fertilization. Development 2013; 140(12): 4471-4479.