Veterinary Research Forum
Vet Res Forum

Effect of chlorogenic acid on follicular development, hormonal status and biomarkers of oxidative stress in rats with polycystic ovary syndrome

Document Type : Original Article

Authors

Neda Abedpour 1
Masoumeh Zirak Javanmard 1
Mojtaba Karimipour 1
Amirreza Pourmajed Liqvan 2

1 Department of Anatomy, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

2 Student Research Committee, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran

https://doi.org/10.30466/vrf.2021.530203.3179
Abstract
Polycystic ovarian syndrome (PCOS) is a complex endocrine and metabolic disorder. Chlorogenic acid (CGA) bears antioxidant properties with protective effects on different tissues. This study was conducted to evaluate the effect of CGA on follicular development, hormonal status and biomarkers of oxidative stress in a rat model of PCOS. In this experimental study, 18 rats were divided into three equal groups including: control, non-treated PCOS [(estradiol valerate (EV): 40.00 mg kg-1 intramuscularly)], and PCOS-CGA (EV: 40.00 mg kg-1 intramuscularly and CGA: 100 mg kg-1 intraperitoneally once a week for eight consecutive weeks). At the end of treatment period, all rats were anesthetized. Then 5.00 mL blood samples of rats in the three groups were taken and prepared for hormonal analyses and their ovaries were isolated and dissected mechanically free of fat and mesentery. The ovaries underwent the following analyses: Morphological study with Hematoxylin and Eosin staining and biochemical study using the malondialdehyde (MDA) level and total antioxidant activity. Data were analyzed using one-way ANOVA and post hoc Tukey’s test. The serum level of luteinizing hormone, estrogen, testosterone, antioxidant capacity, glutathione and the number of cystic follicles in the PCOS group treated with 100 mg kg-1 Chlorogenic acid compared to the non-treated PCOS group were significantly decreased, however, the serum level of follicle stimulating hormone, progesterone, MDA and the number of secondary, graafian follicles and corpus luteum were significantly increased. Chlorogenic acid could be effective in ameliorating follicular development as well as hormonal and biochemical disorders in rats with PCOS.

Keywords

Chlorogenic acid
Gonadal steroid hormones
Ovarian follicle
Oxidative stress
Polycystic ovarian syndrome
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Veterinary Research Forum
Volume 13, Issue 4
Autumn 2022
Pages 513-520

  • Receive Date 17 May 2021
  • Revise Date 10 July 2021
  • Accept Date 13 September 2021

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