Veterinary Research Forum
Vet Res Forum

Effect of GnRH and hCG injection on the reproductive performance and serum progesterone concentration of ewes during spring season

Document Type : Original Article

Authors

Kolsoom Nikbakht 1
Javad Habibizad 2
Mehrdad Meamar 2

1 MSc Graduate, Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran

2 Department of Animal Science, Faculty of Agriculture, Yasouj University, Yasouj, Iran

https://doi.org/10.30466/vrf.2021.527306.3158
Abstract
This study was carried out to investigate the effect of different hormonal programs on the reproductive parameters and serum progesterone concentration of Lake-Ghashghaei ewes in spring season. In this study, 144 ewes were selected from two large nomadic herds. The ewes were synchronized using progesterone sponges. One day before harvesting the sponges, all ewes were injected intramuscularly with 400 IU of eCG. Then ewes were divided into six groups (12 ewes). In the first herd, 1 (GnRH-1 group), 2 (GnRH-2 group), 5 (GnRH-5 group), 7 (GnRH-7 group) and 12 days (GnRH-12 group) after estrus observation, 25.00 µg GnRH was injected intramuscularly to the ewes. In the second herd, the ewes were received 500 IU of hCG on days 1 (hCG-1), 2 (hCG-2), 5 (hCG-5), 7 (hCG-7) and 12 (hCG-12). In each herd, a group of 12 ewes received only eCG and progesterone sponges and was considered as control group. The results showed that twinning rate, the number of born lambs, fecundity and prolificacy rate were higher GnRH and hCG received groups on the second day compared to other groups. The serum progesterone concentration was significantly higher in all groups received GnRH and hCG compared to control group. In general, the results showed that GnRH and hCG two days after mating improved different reproductive activities in addition to increasing serum progesterone concentration. On the other hand, hCG had a greater effect on progesterone concentration, the number of born lambs and the fecundity rate compared to GnRH.

Keywords

GnRH
hCG
Lake-Ghashghaei ewes
Prolificacy rate
Twining rate
  1. Reynolds LP, Borowicz PP, Caton JS, et al. Utero-placental vascular development and placental function: an update. Int J Dev Biol 2010; 54(2-3): 355-366.
  2. Michels H, Vanmontfort D, Dewil E, et al. Genetic variation of prenatal survival in relation to ovulation rate in sheep: A review. Small Rumin Res 1998; 29(2): 129-142.
  3. Roberts RM, Schalue-Francis T. Maternal recognition of pregnancy and embryonic loss. Theriogenology 1990; 33(1): 175-183.
  4. Kittok RJ, Stellflug JN, Lowry SR. Enhanced progesterone and pregnancy rate after gonadotropin administration in lactating ewes. J Anim Sci 1983; 56(3): 652-655.
  5. Fernandez J, Bruno-Galarraga MM, Soto AT, et al. Hormonal therapeutic strategy on the induction of accessory corpora lutea in relation to follicle size and on the increase of progesterone in sheep. Theriogenology 2018; 105: 184-188.
  6. Coleson MP, Sanchez NS, Ashley AK, et al. Human chorionic gonadotropin increases serum progesterone, number of corpora lutea and angiogenic factors in pregnant sheep. Reproduction 2015; 150(1): 43-52.
  7. Antoniazzi AQ, Webb BT, Romero JJ, et al. Endocrine delivery of interferon tau protects the corpus luteum from prostaglandin F2 alpha-induced luteolysis in ewes. Biol Reprod 2013; 88(6): 144. doi: 10.1095/biolreprod.112.105684.
  8. Fernandez J, Bruno-Galarraga MM, Soto AT, et al. Effect of GnRH or hCG administration on Day 4 post insemination on reproductive performance in Merino sheep of North Patagonia Theriogenology 2019; 126: 63-67.
  9. Hashem NM, El-Azrak KM, Nour El-Din AN, et al. Effect of GnRH treatment on ovarian activity and reproductive performance of low-prolific Rahmani ewes. Theriogenology 2015; 83(2): 192-198.
  10. Khan TH, Beck NF, Khalid M. The effect of hCG treatment on Day 12 post-mating on ovarian function and reproductive performance of ewes and ewe lambs. Anim Reprod Sci 2009; 116(1-2): 162-168.
  11. Akif Cam M, Kuran M. Effects of a single injection of hCG or GnRH agonist on day 12 post mating on fetal growth and reproductive performance of sheep. Anim Reprod Sci 2004; 80(1-2): 81-90.
  12. Bazer FW, Ott TL, Spencer TE. Maternal recognition of pregnancy: Comparative aspects: A review. Placenta 1998; 19(Suppl 2): 375-386.
  13. Hosseinzadeh Aski A, Masoudi R, Zare-Shahneh A, et al. The effect of equine chorionic gonadotrophin (eCG) injection combined with prostaglandin F2α (pGF2α) and gonadotrophin releasing hormone (GnRH) treatment on reproductive performance of Zandi ewes during non-breeding season. Arch Razi Inst 2016; 71(4): 269-276.
  14. Jordan KM, Inskeep EK, Knights M. Use of gonadotropin releasing hormone to improve reproductive responses of ewes introduced to rams during seasonal anestrus. Anim Reprod Sci 2009; 116(3-4): 254-264.
  15. Karaca F, Ataman M, Çoyan K. Synchronization of estrus with short-and long-term progestagen treatments and the use of GnRH prior to short-term progestagen treatment in ewes. Small Rumin Res 2009; 81(2): 185-188.
  16. Silva BDM, Silva TASN, Moreira NH, et al. Ovulation induction in ewes using GnRH in long and short-term synchronization protocols. Anim Reprod Belo Horizonte 2015; 12(2): 312-315.
  17. da Fonseca JF, Castro ACR, Arashiro EKN, et al. Effects of hCG administration on accessory corpus luteum formation and progesterone production in estrous-induced nulliparous Santa Inés ewes. Anim Reprod 2018; 15(2): 135-139.
  18. Quintero J, Olguín H, Quezada A, et al. Effect of hCG application on day 12 post-mating on the reproductive efficiency and plasmatic concentrations of progesterone in hair ewes. Cuba J Agric Sci 2015; 49(4): 487-490.
  19. García-Pintos C, Menchaca A. Pregnancy establishment and maintenance after the administration of equine chorionic gonadotropin (eCG) associated or not with gonadotropin-releasing hormone (GnRH) after insemination in sheep. Anim Prod Sci 2018; 58(10): 1802-1806.
  20. Catalano R, Teruel M, González C, et al. Reproductive performance of ewe lambs in non-breeding season exposed to hCG at day 12 post mating. Small Rumin Res 2015; 124: 63-67.
  21. Dias LMK, Sales JNS, Viau P, et al. Although it induces synchronized ovulation, hCG reduces the fertility of Santa Ines ewes submitted to TAI. Arq Bras Med Vet Zootec 2018; 70(1): 122-130.
  22. Habibizad J, Riasi A, Kohram H, et al. Effect of feeding greater amounts of dietary energy for a short-term with or without eCG injection on reproductive performance, serum metabolites and hormones in ewes. Anim Reprod Sci 2015; 160: 82-89.
  23. Lashari MH, Tasawar Z. The effect of GnRH given on day of mating on ovarian function and reproductive performance in Lohi sheep. Pak Vet J 2010; 30(1): 29-33.
  24. Afri-Bouzebda F, Lamraoui R, Bouzebda Z, et al. Effects of GnRH or hCG on ovarian response in PMSG-superovulated Ouled Djellal ewes (Algeria). Global Veterinaria 2015; 15(5): 498-505.
  25. Stevenson JS, Portaluppi MA, Tenhouse DE, et al. Interventions after artificial insemination: conception rates, pregnancy survival, and ovarian responses to gonadotropin-releasing hormone, human chorionic gonadotropin, and progesterone. J Dairy Sci 2007; 90(1): 331-340.
  26. Fukui Y, Itagaki R, Ishida N, et al. Effect of different hCG treatments on fertility of estrus-induced and artificially inseminated ewes during the non-breeding season. J Reprod Dev 2001; 47(4): 189-5.
  27. Ishida N, Okada M, Sebata K, et al. Effects of GnRH and hCG treatments for enhancing corpus luteum function to increase lambing rate of ewes artificially inseminated during the non-breeding season. J Reprod Dev 1999; 45(1): 73-79.
  28. Khan TH, Beck NF, Khalid M. The effects of GnRH analogue (buserelin) or hCG (chorulon) on Day 12 of pregnancy on ovarian function, plasma hormone concentrations, conceptus growth and placentation in ewes and ewe lambs. Anim Reprod Sci 2007; 102(3-4): 247-257.
  29. Khan TH, Hastie PM, Beck NF, et al. hCG treatment on day of mating improves embryo viability and fertility in ewe lambs. Anim Reprod Sci 2003; 76(1-2): 81-89.
  30. Rostami B, Hajizadeh R, Shahir MH, et al. The effect of post-mating hCG or progesterone administration on reproductive performance of Afshari× Booroola-Merino crossbred ewes. Trop Anim Health Prod 2017; 49(2): 245-250.
  31. Ambrose JD, Pires MF, Moreira F, et al. Influence of deslorelin (GnRH-agonist) implant on plasma progesterone, first wave dominant follicle and pregnancy in dairy cattle. Theriogenology 1998; 50(7): 1157-1170.
  32. Vasconcelos JL, Sartori R, Oliveira HN, et al. Reduction in size of the ovulatory follicle reduces subsequent luteal size and pregnancy rate. Theriogenology 2001; 56(2): 307-314.
  33. Yavas Y, Walton JS. Induction of ovulation in postpartum suckled beef cows: a review. Theriogenology 2000; 54(1): 1-23.
  34. Hafez B, Hafez ESE. Reproduction in farm animals. 7th Philadelphia, USA: Lippincott Willimas and Wilkins 2000; 509.
  35. Binelli M, Thatcher WW, Mattos R, et al. Antiluteolytic strategies to improve fertility in cattle. Theriogenology 2001; 56(9): 1451-1463.
  36. Aslan S, Arslanbas D, Beindorff N, et al. Effects of induction of ovulation with GnRH or hCG on follicular and luteal blood flow in Holstein-Friesian heifers. Reprod Domest Anim 2011; 46(5): 781-786.
  37. Cole LA. hCG, the wonder of today’s science. Reprod Biol Endocrinol 2012; 10: 24. doi: 10.1186/1477-7827-10-24.
  38. Gomez-Brunet A, Santiago-Moreno J, Montoro V, et al. Reproductive performance and progesterone secretion in estrus-induced Manchega ewes treated with hCG at the time of AI. Small Rumin Res 2007; 71: 117-122.
  39. Kaya S, Kaçar C, Kaya D, et al. The effectiveness of supplemental administration of progesterone with GnRH, hCG and PGF2α on the fertility of Tuj sheep during the non-breeding season. Small Rumin Res 2013; 113(2-3): 365-370.
  40. Nephew KP, Cárdenas H, McClure KE, et al. Effects of administration of human chorionic gonadotropin or progesterone before maternal recognition of pregnancy on blastocyst development and pregnancy in sheep. J Anim Sci 1994; 72(2): 453-458.
  41. Schmitt EJ, Barros CM, Fields PA, et al. A cellular and endocrine characterization of the original and induced corpus luteum after administration of a gonadotropin-releasing hormone agonist or human chorionic gonadotropin on day five of the estrous cycle. J Anim Sci 1996; 74(8): 1915-1929.
  42. Reynolds LP, Vonnahme KA, Lemley CO, et al. Maternal stress and placental vascular function and remodeling. Curr Vasc Pharmacol 2013; 11(5): 564-593.
  43. Cam MA, Kuran M, Yildiz S, et al. Fetal growth and reproductive performance in ewes administered GnRH agonist on day 12 post-mating. Anim Reprod Sci 2002; 72(1-2): 73-82.
Veterinary Research Forum
Volume 13, Issue 4
Autumn 2022
Pages 553-561

  • Receive Date 28 March 2021
  • Revise Date 10 July 2021
  • Accept Date 13 September 2021

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