Veterinary Research Forum
Vet Res Forum

Royal jelly protects male mice against nicotine-induced reproductive failure

Document Type : Original Article

Authors

Farnam Azad 1
Vahid Nejati 1
Ali Shalizar-Jalali 2
Gholamreza Najafi 2
Fatemeh Rahmani 1

1 Department of Biology, Faculty of Sciences, Urmia University, Urmia, Iran

2 Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2018.32088
Abstract
This study evaluated the possible protective effect of royal jelly (RJ) on sperm parameters and sperm malondialdehyde (MDA) concentration and in vitro fertilizing potential in nicotine (NIC) exposed male mice. Thrtiy-six male BALB/c mice were randomly divided into six groups (n = 6). Group 1 received normal saline, group 2 received 100 mg kg-1 per day RJ, groups 3 and 4 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively and groups 5 and 6 received NIC at doses of 0.50 and 1.00 mg kg-1 per day, respectively plus RJ. Caudal epididymal sperm characteristics, lipid peroxidation and in vitro fertilizing capacity and embryo development were evaluated after 35 days. The NIC treatment caused a significant decrease in sperm motility and viability and fertilization rate along with poor blastocyst formation and increased sperm DNA damage and MDA levels. Moreover, the incidences of chromatin abnormality in spermatozoa were significantly higher in NIC-exposed mice than those of control. Nevertheless, RJ treatment improved sperm parameters and in vitro fertilization outcome as well as sperm lipid peroxidation level. Data from the current study suggest that RJ has a potential repro-protective action against NIC-induced sperm abnormalities and embryotoxicity in mice.

Keywords

malondialdehyde
Mice
Nicotine
Royal jelly
Sperm
Subjects

  1.  

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Veterinary Research Forum
Volume 9, Issue 3
Summer 2018
Pages 231-238

  • Receive Date 15 August 2018

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