Veterinary Research Forum
Vet Res Forum

Effects of curcumin on canine semen parameters and expression of NOX5 gene in cryopreserved spermatozoa

Document Type : Original Article

Authors

Parisa Aparnak 1
Adel Saberivand 2

1 PhD Candidate, Department of Clinical Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

https://doi.org/10.30466/vrf.2019.76137.2015
Abstract
Canine seminal plasma contains antioxidant enzymes to protect sperm against internally generated ROS. These enzymes are removed from seminal plasma during the process of cryopreservation. The freezing/thawing process can cause some morphological and functional changes via ice crystallization and osmolality imbalance. The present study was conducted to evaluate the effects of curcumin supplementation on sperm total count, motility, progressive motility, viability, morphology, total antioxidant capacity (TAC), DNA integrityand NOX5 gene expression of dog frozen semen. The pooled semen was allocated to fresh (Group 1) and frozen (Group 2) controls, curcumin (2.50 mM) (Group 3) and curcumin (5.00 mM), (Group 4). Sperm parameters including total sperm count, morphology, motility, progressive motility, sperm concentration and DNA integrity in addition to TAC were evaluated in fresh and frozen-thawed semen samples. Real-time PCR was used to investigate NOX5 and GADPH (reference gene) genes expressions. Curcumin at 2.50 mM provided a greater protective effect on the DNA integrity compared to 5.00 mM and control groups. TAC was significantly higher in 2.50 mM group than other groups. NOX5 gene expression in curcumin 2.50 mM was higher than 5.00 mM group. In conclusion, curcumin seems to emolliate sperm parameters and to protect sperm against sperm reactive oxygen stress and increases NOX5 gene expression.

Keywords

Cryopreservation
Curcumin
Dog
NOX5
Total antioxidant capacity

Subjects

  1.  

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Veterinary Research Forum
Volume 10, Issue 3
Summer 2019
Pages 221-226

  • Receive Date 02 December 2017
  • Revise Date 13 February 2018
  • Accept Date 21 February 2018

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