Veterinary Research Forum
Vet Res Forum

Histopathological evaluation of the role of negative electrical charge on renal ischemia/reperfusion injuries on brain and heart tissues in rat

Document Type : Original Article

Authors

Sara Dehyadegari 1
Mohammad Mehdi Oloumi 2
Shahrzad Azizi 3

1 DVM Graduate, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

3 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran

https://doi.org/10.30466/vrf.2018.86965.2126
Abstract
This study was performed to evaluate the role of electroacupuncture on kidney 1 (Ki1) acupoint to prevent the heart and brain injury following ischemia/reperfusion of both kidneys. 24 Sprague Dawley rats were randomly assigned into four equal groups. In the treatment 1 group, following anesthesia, acupuncture needles were inserted on Ki1 on the palm of both hindlimbs and connected to electroacupuncture unit for a 3.00 Hz direct current, 1 hr before surgery until the end of surgery. In treatment two groups, the electroacupuncture was also performed 48 and 24 hr before the operation, with the same protocol as treatment 1. Control 1 and control 2 groups had the same procedures like the treatment ones, except for acupuncture. Immediately after reperfusion, the samples of brains and hearts were taken and prepared for microscopic examination. Histopathological study of the heart in the control and treatment groups showed the breakage of myofibrils, hyaline necrosis, edema and disorganization of myocytes. The severity of cardiac lesions was decreased in both treatment groups in comparison with the controls. Brain in control and treatment groups showed ischemic necrosis, disorganization of the neurons in the hippocampus, and edema. The severity of lesions was reduced in the treatment groups and showed a significant difference between the control and treatment 1. It could be concluded that electroacupuncture on the Ki1 point could reduce the severity of damages induced by renal ischemia/reperfusion in the remote organs of the heart and brain.

Keywords

Brain
Electroacupuncture
Heart
Ischemia-reperfusion injury
Kidney

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Veterinary Research Forum
Volume 11, Issue 1
Winter 2020
Pages 15-20

  • Receive Date 13 June 2018
  • Revise Date 04 December 2018
  • Accept Date 29 December 2018

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