Veterinary Research Forum
Vet Res Forum

In vitro sperm storage with poultry oviductal secretions

Document Type : Original Article

Authors

Ana Karen Vargas Ibarra 1
Samantha Anahi Carcoba Pérez 1
Alejandro Avalos Rodríguez 2
Ana María Rosales Torres 2
Fernanda Rodriguez-Hernandez 3
Ricardo Camarillo Flores 4
José Antonio Quintana López 5
José Antonio Herrera Barragán 2

1 Metropolitan Autonomous University - X, Mexico City, Mexico

2 Department of Agricultural and Animal Production, Metropolitan Autonomous University - X, Mexico City, Mexico

3 Department of Agricultural and Animal Production. Metropolitan Autonomous University Xochimilco

4 Metropolitan Autonomous University - I, Mexico City, Mexico

5 Department of Avian Medicine and Zootechnics, College of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Mexico City, Mexico

https://doi.org/10.30466/vrf.2019.95854.2300
Abstract
In the hen oviduct, tubules have been identified that preserve the sperm, maintaining viability for up to 15 weeks. This study aimed to evaluate the physiological status of rooster sperm when preserved in vitro with uterus vaginal junction secretions (UVJS). Males and females of the Rhode Island breed were used. Sperm aliquots were prepared using Lake extender and Lake extender with UVJS (10.00%, 30.00%, 60.00%, and 90.00%). Subsequently, a basic sperm evaluation was performed and sperm physiological status was determined through the presence and distribution of Ca2+ and its acrosomal reaction capability via perivitelline layer (PVL) co-incubation. It was observed that motility was decreased in sperm preserved with UVJS at 6 and 24 hr) compared to 40 min and fresh semen. The sperm decapacitation percentage was increased when preserved with UVJS at 40 min, 6 and 24 hr compared to fresh semen. The acrosomal reaction was increased in sperm co-incubated with PVL, even when preserved with UVJS. It was concluded that UVJS induced physiological changes in sperm by inducing a decapacitation process, which increased sperm viability when preserved in vitro.

Keywords

Acrosome
Decapacitation
Hen
Oviduct
Semen

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Veterinary Research Forum
Volume 11, Issue 3
Summer 2020
Pages 207-211

  • Receive Date 24 October 2018
  • Revise Date 16 January 2019
  • Accept Date 02 February 2019

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