Veterinary Research Forum
Vet Res Forum

The effectiveness of Teucrium chamaedrys L. extracts on endometriotic ‎implant regression in rat endometriosis model

Document Type : Original Article

Authors

Sule Ozel 1
Ipek Suntar 2
Nilufer Ercan Gokay 1
Tugba Taskin Turkmenoglu 3
Murside Ayse Demirel 4

1 Clinic of Gynecology, Zekai Tahir Burak Woman’s Health Training and Research Hospital, University of Health Sciences, Ankara, Turkey

2 Department of Pharmacognosy, Faculty of Pharmacy, Gazi University, Ankara, Turkey

3 Department of Pathology, Diskapi Yildirim Beyazit Training and Research Hospital, Ankara, Turkey

4 Laboratory Animals Care and Research Unit, Department of Pharmacology, Faculty of Pharmacy, Gazi University, Ankara, Turkey

https://doi.org/10.30466/vrf.2019.105229.2500
Abstract
The aim of the present study was to investigate the therapeutic effects of Teucrium chamaedrys L. (Lamiaceae) in the experimentally induced endometriosis in rats. Endometrial tissue was implanted into the abdominal wall of thirty Sprague Dawley rats; the rats with endometriosis were randomized into five groups and treatment procedure was performed for three weeks. The treatment groups were orally treated with three different extracts of Teucrium chamaedrys. Buserelin acetate (20.00 mg) was given as a reference drug. Vehicle was administered alone to the control group. All rats were sacrified at the end of the experiment. The endometriotic implants were measured, intra-abdominal adhesions were scored and the tissue samples were histopathologically investigated. After the treatment procedure, the volumes of endometrial implant and adhesions were detected to be significantly decreased in the T. chamaedrys extracts treated groups compared to the control group. Therapeutic effect of the T. chamaedrys extracts could be attributed to the both nonpolar and polar secondary metabolites. The study conceived that the different polarity extracts of T. chamaedrys could be beneficial in the treatment of endometriosis.

Keywords

‎ Endometriosis
‎ Lamiaceae
‎ Rat
‎ Teucrium chamaedrys
  1. Leyland N, Casper R, Laberge P, et al. Endometriosis: diagnosis and management. J Obstet Gynaecol Can 2010;32(7 Suppl 2):S1-S32.
  2. Dayangan Sayan C, Özakşit MG, Sarıkaya E, et al Serum interleukin-8, CA-125 levels, neutrophil-to-lymphocyte ratios, and combined markers in the diagnosis of endometriosis. Turk J Med Sci 2013;43: 417-423.
  3. Măluțan AM, Drugan T, Ciortea R, et al. Endometriosis-associated changes in serum levels of interferons and chemokines. Turk J Med Sci 2017;47(1):115-122.
  4. Moreira LLQ, de Carvalho ECQ, Caldas-Bussiere MC. Differential immunohistochemical expression of matrix metalloproteinase-2 and tissue inhibitor of metallo-proteinase-2 in cow uteri with adenomyosis during follicular phase. Vet Res Commun 2011; 35:261-269.
  5. Demirel MA. A case of spontaneous abortion related to ovarian endometriosis in a Golden Retriever dog. Iran J Vet Res 2017;18(1):63-66.
  6. Giudice LC, Kao LC. Endometriosis. Lancet 2004; 364(9447):1789–1799.
  7. Arruda MS, Petta CA, Abrão MS, et al. Time elapsed from onset of symptoms to diagnosis of endometriosis in a cohort study of Brazilian women. Hum Reprod. 2003;18(4):756-759.
  8. Schlafer DH. Equine endometrial biopsy: enhancement of clinical value by more extensive histopathology and application of new diagnostic techniques. Theriogenology 2007; 68(3):413-422.
  9. Perez-Marin CC, Molina L, Dominguez JM, et al. Incidental finding of uterine adenomyosis in a bitch with reproductive disorders: a case report. Vet Med (Praha) 2008; 53(11):636-640.
  10. Bulman-Fleming J. A rare case of uterine adenomyosis in a Siamese cat. Can Vet J 2008; 49(7):709-712.
  11. Ayers JW, Birenbaum DL, Menon KM. Luteal phase dysfunction in endometriosis: elevated progesterone levels in peripheral and ovarian veins during the follicular phase. Fertil Steril 1987; 47(6): 925-929.
  12. Pluchino N, Drakopoulos P, Wenger JM, et al. Endocrinology of pregnancy loss. In: Carp HJA (Ed). Recurrent pregnancy loss: Causes, controversies, and treatment. 2nd Ed. New York, USA: CRC Press 2014; 111-121.
  13. Pfeifer S, Fritz M, Goldberg J, et al. Endometriosis and infertility: a committee opinion. Fertil Steril 2012; 98(3):591-598.
  14. Rice VM. Conventional medical therapies for endometriosis. Ann N Y Acad Sci 2002; 955:343-352.
  15. Ricci AG, Olivares CN, Bilotas MA, et al. Natural therapies assessment for the treatment of endometriosis. Hum Reprod 2013; 28:178-188.
  16. Rader JI, Delmonte P, Trucksess MW. Recent studies on selected botanical dietary supplement ingredients. Anal Bioanal Chem 2007; 389(1):27-35.
  17. Herrera S, Bruguera M. Hepatotoxicity induced by herbs and medicines used to induce weight loss [Spanish]. Gastroenterol Hepatol 2008;31(7):447-453.
  18. Bedir E, Manyam R, Khan I.A. Neo-clerodane diterpenoids and phenylethanoid glycosides from Teucrium chamaedrys L. Phytochemistry 2003;63(8): 977-983.
  19. Lin LZ, Harnly JM, Upton R. Comparison of the phenolic component profiles of skullcap (Scutellaria lateriflora) and germander (Teucrium canadense and T. chamaedrys), a potentially hepatotoxic adulterant. Phytochem Anal 2009;20(4):298-306.
  20. Vernon MW, Wilson EA. Studies on the surgical induction of endometriosis in the rat. Fertil Steril 1985; 44(5):684-694.
  21. Altintas D, Kokcu A, Tosun M, et al. Comparison of the effects of cetrorelix, a GnRH antagonist, and leuprolide, a GnRH agonist, on experimental endometriosis. J Obstet Gynaecol Res 2008;34(6):1014-1019.
  22. Blauer KL, Collins RL. The effect of intraperitoneal progesterone on postoperative adhesion formation in rabbits. Fertil Steril.1988;49:144-149.
  23. Taylor HS, Osteen KG, Bruner-Tran KL, et al. Novel therapies targeting endometriosis. Reprod Sci 2011;18(9):814-823.
  24. Abbas MA, Taha MO, Disi AM, et al. Regression of endometrial implants treated with vitamin D3 in a rat model of endometriosis. Eur J Pharmacol 2013; 715(1-3):72-75.
  25. Yildirim B, Guler T, Akbulut M, et al. 1-alpha,25-dihydroxyvitamin D3 regresses endometriotic implants in rats by inhibiting neovascularization and altering regulation of matrix metalloproteinase. Postgrad Med 2014;126(1):104-110.
  26. Shinwari S, Ahmad M, Zhang G, et al. Medicinal plant diversity use for gynecological disorders among the rural communities of Northern Pakistan. Pak J Bot 2017; 49(5):1787-1799.
  27. Vlase L, Benedec D, Hanganu D, et al. Evaluation of antioxidant and antimicrobial activities and phenolic profile for Hyssopus officinalis, Ocimum basilicum and Teucrium chamaedrys.Molecules 2014; 19(5):5490-5507.
  28. Abadian K, Keshavarz Z, Mojab F, et al. The biological effects of Teucrium polium on the severity of primary dysmenorrhea. J Appl Biotechnol Rep 2016; 3(3):453-456.
  29. Seeff L, Stickel F, Navarro VJ. Hepatotoxicity of herbals and dietary supplements. In, Kaplowitz N, DeLeve LD (Eds). Drug-induced liver disease. 3rd ed. Amsterdam, The Netherland: Elsevier 2013; 631-658.
Veterinary Research Forum
Volume 11, Issue 4
Autumn 2020
Pages 305-309

  • Receive Date 15 March 2019
  • Revise Date 22 May 2019
  • Accept Date 06 July 2019

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