Document Type : Short Communication


1 Department of Biochemisty, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, Hatay, Turkiye

2 Department of Biochemistry, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkiye

3 Department of Pathology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkiye


Epidermal growth factor (EGF) has biological roles, including embryonic organ development, breast morphogenesis, breast cell proliferation, and mammary development. This study aimed to measure EGF concentration and evaluate its relationship with somatic cell count (SCC) in healthy water buffaloes (Bubalus bubalis) milk. The study material was constituted of 120 milk samples obtained from 30 healthy water buffaloes between the ages of 3 - 6 years, negative for California mastitis test and SCC less than 3.00 × 105 cells mL-1 milk. In milk serum samples, the EGF concentration was measured using a bovine-specific enzyme-linked immunosorbent assay kit. Epidermal growth factor concentration in the buffalo milk was ranged from 4.30 to 9.80 ng mL-1, with a mean of 8.30 ± 1.50 ng mL-1. Positive correlation between milk SCC values and EGF concentrations was recorded in water buffaloes. Further research is required to evaluate the content of milk EGF in different species of animals because of the EGF effective role in mammary gland and intestinal mucosa.


  1. Collier RJ, McGrath MF, Byatt JC, et Regulation of bovine mammary gland growth by peptide hormones: involvement of receptors, growth factors and binding proteins. Livest Prod Sci 1993; 35(1-2): 21-33.
  2. Dvorak B. Milk epidermal growth factor and gut protection. J Pediatr 2010; 156(2 Suppl): S31-S35.
  3. Akers RM. Lactation physiology: a ruminant animal perspective. Protoplasma 1990; 159: 96-111.
  4. Dehnhard M, Claus R, Munz O, et al. Course of epidermal growth factor (EGF) and insulin-like growth factor I (IGF-I) in mammary secretions of the goat during end-pregnancy and early lactation. J Vet Med A Physiol Pathol Clin Med 2000; 47(9): 533-540.
  5. Sheffield LG. Mastitis increases growth factor messenger ribonucleic acid in bovine mammary glands. J Dairy Sci 1997; 80(9): 2020-2024.
  6. Hård AL, Nilsson AK, Lund AM, et al. Review shows that donor milk does not promote the growth and development of preterm infants as well as maternal milk. Acta Paediatr 2019; 108(6): 998-1007.
  7. Dvorak B, Halpern MD, Holubec H, et al. Epidermal growth factor reduces the development of necrotizing enterocolitis in a neonatal rat model. Am J Physiol Gastrointest Liver Physiol 2002; 282(1): G156-G164.
  8. Okuyama H, Urao M, Lee D, et al The effect of epidermal growth factor on bacterial translocation in newborn rabbits. J Pediatr Surg 1998; 33(2): 225-228.
  9. Alhussien MN, Dang AK. Milk somatic cells, factors influencing their release, future prospects, and practical utility in dairy animals: An overview. Vet World 2018; 11(5): 562-577.
  10. Gokceoglu A, Yarim GF, Gultiken N, et al. High epidermal growth factor concentration associated with somatic cell count in milk of cows with subclinical mastitis. Med Weter 2020; 76(6): 354-357.
  11. Sarfraz A. Buffalo Milk. In: Park YW, Haenlein GFW (Eds). Milk and dairy products in human nutrition: production, composition and health. New York, USA: John Wiley & Sons, Ltd. 2013; 519-553.
  12. Sharma N, Singh NK, Bhadwal MS. Relationship of somatic cell count and mastitis: an overview. Asian-Aust J Anim Sci 2011; 24(3): 429-438.
  13. Kilicoglu C, Alacam E, Izgür H, et al. Udder health control of dairy cows in the area of Ankara (Turkey), [German]. Dtsch Tierarztl Wochenschr. 1989; 96(10): 486-488.
  14. Alais C. Milk science; principles of dairy techniques [French]. 3rd Paris, France: SEPEC 1974; 636-665.
  15. Jaeger LA, Lamar CH, Tottoms GD, et al. Growth-stimulating substances in porcine milk. Am J Vet Res 1987; 48(10): 1531-1533.
  16. Murray MJ, Schaudies RP, Cavey DM. Epidermal growth factor-like activity in mares' milk. Am J Vet Res 1992; 53(10): 1729-1731.
  17. Jansson L, Karlson FA, Westermark B. Mitogenic activity and epidermal growth factor content in human milk. Acta Paediatr Scand 1985; 74(2): 250-253.
  18. Iacopetta BJ, Grieu F, Horisberger M, et al. Epidermal growth factor in human and bovine milk. Acta Paediatr 1992; 81(4): 287-291.
  19. Xiao X, Xiong A, Chen X, et al. Epidermal growth factor concentrations in human milk, cow's milk and cow's milk-based infant formulas. Chin Med J (Engl) 2002; 115(3): 451-454.
  20. Gurler H, Yarım GF, Salar S, et al. Investigation of epidermal growth factor (EGF) levels in milk of Anatolian buffaloes with subclinical mastitis. Kocatepe Vet J 2019; 12(3): 254-257.