Veterinary Research Forum
Vet Res Forum

Rapid and visual detection of an isolated and identified goose parvovirus (GPV) strain by a loop-mediated isothermal amplification assay

Document Type : Original Article

Authors

Jun-Ting Liu 1
Yan-Hui Chen 1
Yi-Feng Pei 1
Qian Yu 1
Ruth Afumba 1
Hao Dong 1, 2

1 Department of Microbiology, Faculty of Life Sciences, Jilin Agricultural University, Changchun, China

2 Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, China

https://doi.org/10.30466/vrf.2021.540351.3246
Abstract
Gosling plague caused by goose parvovirus (GPV), a highly infectious septic disease with high mortality, has caused substantial loss in the waterfowl industry. A method for the rapid detection of GPV is needed. In this study, we isolated the virus strain of GPV in May 2020 and applied it to the loop-mediated isothermal amplification (LAMP) assay. We designed five sets of primers for the goose parvovirus VP3 gene by LAMP. The GV-1 primer set was selected to detect GPV sensitively and rapidly. LAMP was more sensitive compared to PCR. In addition, the LAMP method could complete detection within 60 min which was faster than the PCR assay. The LAMP provided a convenient and effective experimental method for detection of GPV for inspection and quarantine departments and health care units in China, and it is expected to become a simple and routine detection method, especially suitable for goose farms.

Keywords

Goose parvovirus
Gosling plague
Loop-mediated isothermal amplification
  1. Li P, Zhang R, Chen J, et al. Development of a duplex semi-nested PCR assay for detection of classical goose parvovirus and novel goose parvovirus-related virus in sick or dead ducks with short beak and dwarfism syndrome. J Virol Methods 2017; 249: 165-169.
  2. Bian G, Ma H, Luo M, et al. Identification and genomic analysis of two novel duck-origin GPV-related parvovirus in China. BMC Vet Res 2019; 15: 88. doi: 10.1186/s12917-019-1833-9.
  3. Ning K, Liang T, Wang M, et al. Genetic detection and characterization of goose parvovirus: Implications for epidemiology and pathogenicity in Cherry Valley Pekin ducks. Infect Genet Evol 2017; 51: 101-103.
  4. Wang J, Huang Y, Zhou M, et al. Construction and sequencing of an infectious clone of the goose embryo-adapted Muscovy duck parvovirus vaccine strain FZ91-30. Virol J 2016; 13: 104. doi: 10.1186/s12985-016-0564-9.
  5. Wang Q, Ju H, Li Y, et al. Development and evaluation of a competitive ELISA using a monoclonal antibody for antibody detection after goose parvovirus virus-like particles (VLPs) and vaccine immunization in goose sera. J Virol Methods 2014; 209: 69-75.
  6. Shao H, Lv Y, Ye J, et al. Isolation of a goose parvovirus from swan and its molecular characteristics. Acta Virol 2014; 58(2): 194-198.
  7. Niu Y, Zhao L, Liu B, et al. Comparative genetic analysis and pathological characteristics of goose parvovirus isolated in Heilongjiang, China. Virol J 2018; 15(1): 27. doi: 10.1186/s12985-018-0935-5.
  8. Poonia B, Dunn PA, Lu H, et al. Isolation and molecular characterization of a new Muscovy duck parvovirus from Muscovy ducks in the USA. Avian Pathol 2006; 35(6): 435-441.
  9. Yan YQ, He TQ, Li R, et al. Molecular characterization and comparative pathogenicity of goose parvovirus isolated from Jilin province, northeast China. Avian Dis 2019; 63(3): 481-485.
  10. Niu X, Chen H, Yang J, et al. Development of a TaqMan-based real-time PCR assay for the detection of Novel GPV. J Virol Methods 2016; 237: 32-37.
  11. Liu Z, Zhang Q, Yang NN, et al. Rapid and sensitive detection of salmonella in chickens using loop-mediated isothermal amplification combined with a lateral flow dipstick. J Microbiol Biotechnol 2019; 29(3): 454-464.
  12. Yu X, Wei L, Chen H, et al. Development of colloidal gold-based immunochromatographic assay for rapid detection of goose parvovirus. Front Microbiol 2018; 9: 953. doi: 10.3389/fmicb.2018.00953.
  13. Zhang S, Yang J, Wang Z, et al. Research Note: Development of an ELISA to distinguish between goose parvovirus infection and vaccine immunization antibodies. Poult Sci 2020; 99(3): 1332-1340.
  14. Walker DI, McQuillan J, Taiwo M, et al. A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters. Water Res 2017; 126: 101-110.
  15. Zhou D, Wang S, Yang K, et al. Rapid and simultaneous detection of Japanese encephalitis virus by real-time nucleic acid sequence-based amplification. Microb Pathog 2021; 150: 104724. doi: 10.1016/j.micpath. 2020.104724.
  16. Notomi T, Okayama H, Masubuchi H, et al. Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 2000; 28: E63. doi: 10.1093/nar/ 28.12.e63.
  17. Yin J, Wang Q, Wang Y, et al. Development of a simple and rapid reverse transcription-loopmediated isothermal amplification (RT‐LAMP) assay for sensitive detection of tilapia lake virus. J Fish Dis 2019; 42(6): 817-824.
  18. Zhao N, Liu J, Sun D. Detection of HCV genotypes 1b and 2a by a reverse transcription loop-mediated isothermal amplification assay. J Med Virol 2017; 89(6): 1048-1054.
  19. Shehata AA, Gerry DM, Heenemann K, et al. Goose parvovirus and circovirus coinfections in ornamental ducks. Avian Dis 2016; 60(2): 516-522.
  20. Xie L, Xie Z, Zhao G, et al. A loop-mediated isothermal amplification assay for the visual detection of duck circovirus. Virol J 2014; 11: 76. doi: 10.1186/1743-422X-11-76.
  21. Yang J, Chen H, Wang Z, et al. Development of a quantitative loop-mediated isothermal amplification assay for the rapid detection of novel goose parvovirus. Front Microbiol 2017; 8: 2472. doi: 10.3389/fmicb. 2017.02472.
  22. Yu Z, Zhang D, Yang K, et al. A simple and rapid diagnostic method to detect new goose astrovirus using reverse-transcription loop-mediated isothermal amplification. 3 Biotech 2020; 10(1): 20. doi: 10.1007/s13205-019-2006-z.
  23. Yang J, Yang R, Cheng A, et al. A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification. Virol J 2010; 7: 14. doi: 10.1186/1743-422X-7-14.
Veterinary Research Forum
Volume 14, Issue 1
January 2023
Pages 7-12

  • Receive Date 05 October 2021
  • Revise Date 20 November 2021
  • Accept Date 20 December 2021

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