Veterinary Research Forum
Vet Res Forum

Association between uterine toxicity induced by chlorpyrifos and downregulation of heparin-binding epidermal growth factor and L-selectin genes

Document Type : Original Article

Authors

Parisa Gheibi 1
Zohre Eftekhari 2
Delaram Doroud 3
Kazem Parivar 1

1 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

2 Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran

3 Department of Quality Control, Research and Production Complex, Pasteur Institute of Iran, Karaj, Iran

https://doi.org/10.30466/vrf.2021.532844.3203
Abstract
Various factors are effective in reducing the fertility rate. This experiment aimed to investigate chlorpyrifos (CPF), an organophosphate, that could alter the structure of the uterus and the molecules involved in parental and fetal. CPF was injected intraperitoneally in thirty mice for five days in a week (six weeks). The animals were euthanized on the 5th day of gestation, then their blood and uterus were collected for biochemical and histopathological assays. Exposure to CPF resulted in a significant reduction in maternal weight gain and the number of litters. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly increased in blood serum of the CPF group compared with the control. The number of uterus glands, endometrium thickness, and the uterine cavity were changed following CPF injection. Additional investigation indicated that the expressions of L-selectin, L-selectin ligand, and heparin-binding epidermal growth factor (HB-EGF) as initial adhesion of mice blastocysts and maternal endometrium biomarkers were downregulated in the CPF group. Nevertheless, any mortality and abnormal clinical symptoms were not observed in the treated mice. This study revealed a potential molecular mechanism of continuous CPF-induced toxicity in fetal-maternal attachment without clinical symptoms.

Keywords

Adhesion molecules
Endometrium
Liver enzymes
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Veterinary Research Forum
Volume 14, Issue 1
January 2023
Pages 45-52

  • Receive Date 04 July 2021
  • Revise Date 19 August 2021
  • Accept Date 13 September 2021

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