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Synchronization of estrus using progesterone injections followed by human menopausal gonadotropin in ewes

Document Type : Original Article

Authors

1 Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Veterinary Research Institute, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Department of Animal Sciences, School of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

Abstract

Serial progesterone injections followed by human menopausal gonadotropin (hMG), instead of equine chorionic gonadotropin (eCG), were used to synchronize estrus in ewes. Shal ewes
(n = 189) were assigned into five groups and each group was divided into two sub-groups to receive gonadotropins including eCG (300 IU; intra-muscular) or hMG (one ampoule; subcutaneously, SC). All ewes received prostaglandin (PG) F2α six days after introducing ram (day 0). Ewes received 0 (control), one, two, three or four injections of progesterone (50.00 mg; SC), 72 hr apart. The first progesterone was injected at the time of PG injection. Ewes in treatment groups received gonadotropins 48 hr after the last progesterone injection. Control group ewes received gonadotropins, at the time of PG injection. Mating was recorded after introducing fertile rams. Data were analyzed using GLM and GENMOD procedures in SAS. The incidence of estrus was less in control and ewes received a single progesterone (34.20%) compared to ewes received two (64.10%), three (81.10%) and four injections (68.40%) of progesterone. Time to estrus was earlier in control (45.70 ± 4.41 hr) than progesterone-treated groups (63.60 ± 1.79 hr). Fertility (51.30%) and fecundity (78.40%) of ewes received three progesterone injections were significantly greater than other progesterone-treated groups. There was no significant difference in reproductive indices between eCG and hMG sub-groups. In conclusion, during the non-breeding season, three injections of progesterone, three days apart, starting six days after ram exposure, in association with hMG, 48 hr after the last progesterone injection, could provide a sound reproductive performance in Shal ewes.

Keywords

References
  1. Hogue DE. Frequent lambing systems. In: Fayez I, Marai M, Owen JB (Eds). New techniques in sheep production. Oxford, UK: Butterworth-Heinemann. 1987; 57-63.
  2. Bartlewski PM, Beard AP, Cook SJ, et al. Ovarian follicular dynamics during anestrus in ewes. J Reprod Fertil 1998; 113(2): 275-285.
  3. Souza CJ, Campbell BK, Baird DT. Follicular dynamics and ovarian steroid secretion in sheep during anoestrus. J Reprod Fertil 1996; 108(1): 101-106.
  4. Robinson TJ. Role of progesterone in the mating behaviour of the ewe. Nature 1952; 170(4322): 373-374.
  5. Fabre-Nys C, Gelez Sexual behavior in ewes and other domestic ruminants. Horm Behav 2007; 52(1): 18-25.
  6. Wildeus S. Current concepts in synchronization of estrus: Sheep and goats. J Anim Sci 2000; 77: 1-14.
  7. Greyling JPC, Brink WCJ. Synchronization of oestrus in sheep: The use of controlled internal drug release (CIDR)S Afr J Anim Sci 1987; 17(3): 128-132.
  8. Godfrey RW, Gray ML, Collins JR. A comparison of two methods of oestrous synchronisation of hair sheep in the tropics. Anim Reprod Sci 1997; 47(1-2): 99-106.
  9. Cortà NG, Abad-Zavaleta J, Bravo-Delgado HR, et al. Effect of fluorogestone acetate on the vaginal micro-biota from ewes Pelibuey in the Papaloapan region. Trop Subtrop Agroecosystems 2013; 16(3): 309-314.
  10. Dutt RH, Casida LE. Alteration of the estrual cycle in sheep by use of progesterone and its effect upon sub-sequent ovulation and fertility. Endocrinology 1948; 43(4): 208-217.
  11. Dutt RH. Induction of estrus and ovulation in anestrual ewes by use of progesterone and pregnant mare serum. J Anim Sci 1953; 12(3): 515-523.
  12. Boland MP, Gordon I. Oestrus and ovulatory response to progesterone-PMSG treatments in anoestrous ewes. J Dept Agric Fish Ireland 1973; 70: 65-70.
  13. Boland MP, Crosby TF, Gordon I. Effect of mating management and PMSG dose on lambing outcome in ewes bred in late anoestrus. J Agric Sci 1981; 97(2): 445-447.
  14. Abecia JA, Forcada F, González-Bulnes A. Hormonal control of reproduction in small ruminants. Anim Reprod Sci 2012; 130(3-4): 173-179.
  15. Menzer C, Schams D. Radioimmunoassay for PMSG and its application to in-vivo studies. J Reprod Fertil 1979; 55(2): 339-345.
  16. Murphy BD, Martinuk SD. Equine chorionic gonadotropin. Endocr Rev 1991; 12(1); 27-44.
  17. Soumano K, Lussier JG, Price CA. Levels of messenger RNA encoding ovarian receptors for FSH and LH in cattle during superovulation with equine chorionic gonadotrophin versus FSH. J Endocrinol 1998; 156(2): 373-378.
  18. Bodin L, Drion PV, Remy B, et al. Anti-PMSG antibody levels in sheep subjected annually to oestrus syn-chronisation. Reprod Nutr Dev 1997; 37(6): 651-660.
  19. Roy F, Combes B, Vaiman D, et al. Humoral immune response to equine chorionic gonadotropin in ewes: association with major histocompatibility complex and interference with subsequent fertility. Biol Reprod 1999; 61(1): 209-218.
  20. McGowan MR, Braithwaite M, Jochle W, et al. Super-ovulation of beef heifers with Pergonal (HMG): A dose response trial. Theriogenology 1985; 24(2): 173-184.
  21. Ararooti T, Niasari-Naslaji A, Asadi-Moghaddam B, et al. Superovulatory response following FSH, eCG-FSH and hMG and pregnancy rates following transfer of hatched blastocyst embryos with different diameter and shape in dromedary camel. Theriogenology 2018; 106: 149-156.
  22. Riesenberg S, Meinecke-Tillmann S, Meinecke B. Ultrasonic survey of follicular development following superovulation with a single application of pFSH, eCG or hMG in goats. Small Rumin Res 2001; 40(1): 83-93.
  23. Riesenberg S, Meinecke-Tillmann S, Meinecke B. Ultrasonic study of follicular dynamics following superovulation in German Merino ewes. Theriogenology 2001; 55(4): 847-865.
  24. National Research Council (NRC). Nutrient requirements of small ruminants: sheep, goats, cervids, and new world camelids. Washington DC, USA; National Academy Press 2007.
  25. Evans G, Maxwell WC. Salamons' artificial insemination of sheep and goats.2nd ed. Sydney, Australia: Butterworths 1987; 93-106.
  26. Hashemi M, Safdarian M, Kafi M. Estrous response to synchronization of estrus using different progesterone treatments outside the natural breeding season in ewes. Small Rumin Res 2006; 65(3): 279-283.
  27. Rhodes L, Nathanielsz PW. Comparison of a controlled internal drug release device containing progesterone with intravaginal medroxyprogesterone sponges for estrus synchronization in ewes. Theriogenology 1988; 30(4): 831-836.
  28. Tritschler II JP, Duby RT, Parsons EM, et al. Comparison of two progestogens during out-of-season breeding in a commercial ewe flock. Theriogenology 1991; 35(5): 943-952.
  29. Niasari-Naslaji A, Soukhtezari. Comparison between three estrus synchronization programs using progestagens during the breeding season in the ewe [Persian]. Pajouhesh-va-Sazandegi 2005; 65: 86-91.
  30. Windorski EJ, Schauer CS, Wurst AK, et al. Effects of melengestrol acetate and P.G. 600 on fertility in Rambouillet ewes outside the natural breeding season. Theriogenology 2008; 70(2): 227-232.
  31. Swelum AA, Alowaimer AN, Abouheif MA. Use of fluorogestone acetate sponges or controlled internal drug release for estrus synchronization in ewes: Effects of hormonal profiles and reproductive performance. Theriogenology 2015; 84(4): 498-503.
  32. Martinez-Ros P, Lozano M, Hernandez F, et al. Intravaginal device-type and treatment-length for ovine estrus synchronization modify vaginal mucus and microbiota and affect fertility. Animals (Basel) 2018; 8(12): 226. doi: 10.3390/ani8120226
  33. Manes J, Ríos G, Fiorentino MA, et al. Vaginal mucus from ewes treated with progestogen sponges affects quality of ram spermatozoa. Theriogenology 2016; 85(5): 856-861.
  34. Ungerfeld R, Rubianes E. Effectiveness of short-term progestogen primings for the induction of fertile oestrus with eCG in ewes during late seasonal anoestrus. Anim Sci J 1999; 68(3) 349-353.
  35. Niasari-Naslaji A, Seidi-Samani H, Shojaie A, et al. Induction of estrus and fertility of Shal ewes using pro-gesterone injections or intravaginal sponge followed by laparoscopic AI during non-breeding season. Iran Vet J 2022; doi: 10.22055/IVJ. 2021.300556.2389.
  36. Oldham CM, Martin GB. Stimulation of seasonally anovular Merino ewes by rams. II. Premature regression of ram-induced corpora lutea. Anim Reprod Sci 1979; 1(4): 291-295.
  37. Martin GB, Oldham CM, Lindsay DR. Increased plasma LH levels in seasonally anovular Merino ewes following the introduction of rams. Anim Reprod Sci 1980; 3(2) 125-132.
  38. Knight TW, Peterson AJ, Payne E. The ovarian and hormonal response of the ewe to stimulation by the ram early in the breeding season. Theriogenology 1978; 10(5): 343-353.
  39. Knight TW, Tervit HR, Fairclough RJ. Corpus luteum function in ewes stimulated by rams. Theriogenology 1981; 15(2): 183-190.
  40. Husein MQ, Bailey MT, Ababneh MM, et al. Effect of eCG on the pregnancy rate of ewes transcervically inseminated with frozen-thawed semen outside the breeding season. Theriogenology 1998; 49(5): 997-1005.
  41. Fukui Y, Akaike M, Anzai H, et al. Effect of timing of injection with pregnant mare's serum gonadotrophin on fixed-time artificial insemination of seasonally anoestrous ewes. J Agric Sci 1989; 113(3): 361-364.
  42. Abecia JA, Forcada F, González-Bulnes A. Pharma-ceutical control of reproduction in sheep and goats. Vet Clin North Am Food Anim Pract 2011; 27(1): 67-79.
  43. Acritopoulou S, Haresign W. Response of ewes to a single injection of an analog of PGF-2 alpha given at different stages of the oestrous cycle. J Reprod Fertil 1980; 58(1): 219-221.
  44. Fierro S, Olivera-Muzante J, Gil J, et al. Effects of prostaglandin administration on ovarian follicular dynamics, conception, prolificacy, and fecundity in sheep. Theriogenology 2011; 76(4): 630-639.
  45. Olivera-Muzante J, Fierro S, López V, et al. Comparison of prostaglandin- and progesterone-based protocols for timed artificial insemination in sheep. Theriogenology 2011; 75(7): 1232-1238.
  46. Boland MP, Gordon I, Kelleher D. The effect of treatment by prostaglandin analog (ICI-80, 996) or progestagen (SC-9880) on ovulation and fertilization in cyclic ewes. J Agric Sci 1978; 91(3), 727-730.
  47. Davies KL, Bartlewski PM, Epp T, et al. Does injection of prostaglandin F(2alpha) (PGF2alpha) cause ovulation in anestrous Western White Face ewes? Theriogenology 2006; 66(2): 251-259.
  48. Hawk HW, Cooper BS. Sperm transport into the cervix of the ewe after regulation of estrus with prostaglandin or progestogen. J Anim Sci 1977; 44(4): 638-644.
  49. Rubianes E, Beard A, Dierschke DJ, et al. Endocrine and ultrasound evaluation of the response to PGF 2alpha and GnRH given at different stages of the luteal phase in cyclic ewes. Theriogenology 1997; 48(7): 1093-1104.
  50. Hawk HW, Cooper BS, Pursell VG. Increased sperm death in the cervix and uterus of estrous ewes after regulation of estrus with prostaglandin or progestogen. J Anim Sci 1981; 52(3): 601-610.
Veterinary Research Forum
Volume 14, Issue 3
March 2023
Pages 145-151
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History
  • Receive Date: 04 December 2021
  • Revise Date: 22 January 2022
  • Accept Date: 08 February 2022
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