Document Type : Original Article


1 DVSc Graduate, Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran


Intestinal hypomotility cause health risks and economic losses and is considered as an important digestive disorder that efforts to find prokinetic drugs can solve this major problem. This study investigated the effects of Zingiber officinale aqueous extract (ZOAE) on caecal smooth muscle contractions in healthy cows. To perform in vitro tests, cecum strips connected to the organ bath. Ginger aqueous extract caused concentration-dependent contraction in caecal smooth muscle with an effective threshold concentration of 6.00 mg L-1. The strongest contraction was caused at a concentration of 100 mg L-1 with an average contraction of 141%. To evaluate the possible mechanisms underlying the contractile effect on cecum strips, atropine, 1,1-dimethyl-4-diphenylacetoxypiperidinium iodide (4-DAMP) and verapamil completely inhibited aqueous extract induced smooth muscle contractions, while addition of hexamethonium had no effect on the contraction process. The lack of reduction of contractions caused by the extract in the presence of hexamethonium indicates that presence of acetylcholine-like constituents independent of nicotinic receptors. The inhibitory properties of atropine and 4-DAMP indicate that at least part of the prokinetic effect of the extract is due to stimulating the muscarinic receptors, especially M3 receptors. Also, verapamil inhibitory function proves that the extract acting by L-type calcium channels. The results suggest that the ZOAE has a potential prokinetic effect which may provide a pharmacological base to its medicinal or prophylactic use in caecal motility disorders.


  1. Braun U, Beckmann C, Gerspach C, et a Clinical findings and treatment in cattle with caecal dilatation. BMC Vet Res 2012; 8: 75. doi: 10.1186/1746-6148-8-75.
  2. Singh B, Kumar A, Udehiya PK, et al. Clinical study on the ultrasonographic features, prognostic indicators and outcome of caecal dilatation in bovine. Indian J Vet Surg 2019; 40(2): 103-106.
  3. Singh G, Udehiya RK, Mohindroo J, et al. Differential diagnosis and surgical management of cecal dilatation vis-a-vis cecal impaction in bovine. Vet world 2018; 11(9): 1244-1249.
  4. Khalphallah A, Elsayed HK, Elmeligy E, et al. Differential diagnosis of bovine intestinal diseases and their sequelae regarding ultrasonography and other diagnostic tools. Vet World 2021; 14(6): 1537-1547.
  5. Steiner A, Roussel AJ, Martig J. Effect of bethanechol, neostigmine, metoclopramide, and propranolol on myoelectric activity of the ileocecocolic area in cows. Am J Vet Res 1995; 56(8): 1081-1086.
  6. Mendel M, Chłopecka M, Dziekan N, et al. Interactions between erythromycin, flunixin meglumine, levamisole and plant secondary metabolites towards bovine gastrointestinal motility-in vitro study. J Vet Pharmacol Ther 2018; 41(2): 281-291.
  7. Mahmoud M, Ahmed MA, Wafa MA, et al. Evaluation the effect of metoclopramide on abomasal emptying rate in healthy non-pregnant mixed breed cattle by ultrasound. Benha Vet Med J 2019; 36(1): 310-315.
  8. Steiner A. Modifiers of gastrointestinal motility of cattle. Vet Clin North Am Food Anim Pract 2003; 19(3): 647-660.
  9. Nam Y, Lee JM, Wang Y, et al. The effect of Flos Loni-cerae Japonicae extract on gastro-intestinal motility function. J Ethnopharmacol 2016; 179: 280-290.
  10. Brierley SM, Kelber O. Use of natural products in gastrointestinal therapies. Curr Opin Pharmacol 2011; 11(6): 604-611.
  11. Oka T, Okumi H, Nishida S, et al. Effects of Kampo on functional gastrointestinal disorders. BioPsychoSocial Med 2014; 8: 5. doi:10.1186/1751-0759-8-5.
  12. Mahboubi M. Zingiber officinale essential oil, a review on its composition and bioactivity. Clin Phytosci 2019;5: 6. doi: 10.1186/s40816-018-0097-4.
  13. Borrelli F, Capasso R, Pinto A, et al. Inhibitory effect of ginger (Zingiber officinale) on rat ileal motility in vitro. Life Sci 2004; 74(23): 2889-2896.
  14. Khodaveisi Z, Borzou SR, Mohammadi Y, et al. The effect of inhalation of ginger extract on postoperative nausea, retching and vomiting after laparoscopic cholecystectomy: a randomized clinical trial [Persian]. J Health Care 2019; 21(2): 126-134.
  15. ElRokh el-SM, Yassin NA, El-Shenawy SM, et al. Antihypercholesterolaemic effect of ginger rhizome (Zingiber officinale) in rats. Inflammopharmacology 2010; 18(6): 309-315.
  16. Ali BH, Blunden G, Tanira MO, et al. Some phytochemical, pharmacological and toxicological properties of ginger (Zingiber officinale Roscoe): a review of recent research. Food Chem Toxicol 2008; 46(2): 409-420.
  17. Ghayur MN, Gilani AH. Pharmacological basis for the medicinal use of ginger in gastrointestinal disorders. Dig Dis Sci 2005; 50(10): 1889-1897.
  18. Mamaghani A, Maham M, Dalir-Naghadeh B. Effects of ginger extract on smooth muscle activity of sheep reticulum and rumen. Vet Res Forum 2013; 4(2): 91-97.
  19. Dadfar F, Bahaoddini B. The effect of hydroalcoholic extract of Zingiber officinale rhizomes on mechanical activity of isolated trachea of male rats. J Jahrom Univ Med Sci. 2014; 12(1): 1-7.
  20. Jalilzadeh-Amin G, Maham M, Dalir-Naghadeh B, et al. Effects of Bunium persicum (Boiss.) essential oil on the contractile responses of smooth muscle (an in vitro study). Vet Res Forum 2011; 2(2): 87-96.
  21. Mohseni M, Maham M, Dalir-Naghadeh B,et al. Does Achillea millefolium extracts possess prokinetic effects on the bovine abomasum thourgh M3 muscarinic receptors? Vet Res Forum 2017; 8(2): 115-120.
  22. Arbati A, Maham M, Dalir-Naghadeh B, The effect of cinnamaldehyde on the contractility of bovine isolated gastrointestinal smooth muscle preparations. Vet Res Forum 2021; 12(3): 313-318.
  23. Najafzadeh H. Effects of Zingiber officinale on contraction of muscles of broiler`s ileum. DVM Thesis. Urmia University. Urmia, Iran: 2015.
  24. Ghayur MN, Gilani AH. Species differences in the prokinetic effects of ginger. Int J Food Sci Nutr 2006; 57(1-2): 65-73.
  25. Ontsouka EC, Niederberger M, Steiner A, et al. Binding sites of muscarinic and adrenergic receptors in gastrointestinal tissues of dairy cows suffering from left displacement of the abomasum. Vet J 2010; 186(3): 328-337.
  26. Poole DP, Littler RA, Smith BL, et al. Effects and mechanisms of action of the ergopeptides ergotamine and ergovaline and the effects of peramine on reticulum motility of sheep. Am J Vet Res 2009; 70(2): 270-276.
  27. Racké K, Juergens UR, Matthiesen S. Control by cholinergic mechanisms. Eur J Pharmacol 2006; 533(1-3): 57-68.
  28. Caulfield MP, Birdsall NJ. International Union of Pharmacology. XVII. Classification of muscarinic acetylcholine receptors. Pharmacol Rev 1998; 50(2): 279-290.
  29. Eglen RM. Muscarinic receptor subtypes in neuronal and non‐neuronal cholinergic function. Auton Autacoid Pharmacol 2006; 26(3): 219-233.
  30. Tanahashi Y, Komori S, Matsuyama H, et al. Functions of muscarinic receptor subtypes in gastrointestinal smooth muscle: a review of studies with receptor-knockout mice. Int J Mol Sci 2021; 22(2):926. doi: 10.3390/ijms22020926.
  31. Lyford GL, Farrugia G. Ion channels in gastrointestinal smooth muscle and interstitial cells of Cajal. Curr Opin Pharmacol 2003; 3(6): 583-587.
  32. Sanders KM, Kito Y, Hwang SJ, et al. Regulation of gastrointestinal smooth muscle function by interstitial cells. Physiology (Bethesda) 2016; 31(5): 316-326.
  33. Eglen RM, Michel AD, Cornett CM, et al. The interaction of hexamethonium with muscarinic receptor subtypes in vitro. Br J Pharmacol 1989; 98(2): 499-506.
  34. Mayer M, Vogl CR, Amorena M, et al. Treatment of organic livestock with medicinal plants: a systematic review of European ethnoveterinary research. Forsch Komplementmed 2014; 21(6): 375-386.
  35. Ayrle H, Mevissen M, Kaske M, et al. Medicinal plants - - prophylactic and therapeutic options for gastro-intestinal and respiratory diseases in calves and piglets? A systematic review. BMC Vet Res 2016; 12:89. doi: 10.1186/s12917-016-0714-8.
  36. Sanhokwe M, Mupangwa J, Masika PJ, et al. Medicinal plants used to control internal and external parasites in goats. Onderstepoort J Vet Res 2016; 83(1):a1016. doi: 10.4102/ojvr.v83i1.1016.