Document Type : Original Article
Department of Animal Science, Faculty of Agriculture, University of Hatay Mustafa Kemal, Hatay, Türkiye
Department of Animal Breeding, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, Hatay, Türkiye
Department of Biochemistry, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, Hatay, Türkiye
Department of Pathology, Faculty of Veterinary Medicine, University of Hatay Mustafa Kemal, Hatay, Türkiye
This research aimed to evaluate the effect of different doses of black cumin (Nigella sativa L.) seed (BCS) on growth performance, blood parameters, liver oxidant/anti-oxidant levels and fatty liver syndrome in quails. Four hundred and thirty-two unsexed (male and female) three-day-old Japanese quail (Coturnix coturnix japonica) chicks were divided into four treatment groups (108 chicks per group) with six replicates (18 chicks per replicate). Control and experimental groups were fed for 35 days with basal quail feed including 0.00, 0.50, 1.00 and 2.00% BCS supplement, respectively. At the end, a total of 96 quails, 24 from each group (12 females and 12 males) were slaughtered. The BCS-addition did not affect the growth performance in the experimental groups compared to the control group. Addition of BCS to the diet significantly decreased serum aspartate aminotransferase, lactate dehydrogenase and urea amounts compared to the controls. Whereas, cholesterol decreased significantly with the addition of only 1.00% and low-density lipoprotein with the addition of 0.50 and 1.00% BCS compared to the controls. Liver glutathione levels significantly elevated in 0.50 and 1.00% BCS fed groups; while, glutathione peroxidase levels significantly decreased in 1.00 and 2.00% BCS fed groups. Adding 1.00 and 2.00% BCS to the feed reduced fatty liver incidence in male quails. It is concluded that adding 0.50 and 1.00% BCS positively affects the blood and liver parameters; therefore, BCS may be suggested as an anti-oxidant source to help protect hepatocytes against tissue damage as it has a significant effect on maintaining oxidant and anti-oxidant balance.
- Çakmakçı S, Çakır Y. Black cumin (Nigella sativa): Composition, its use in food industry and health effects [Turkish]. Acad Food J 2011; 9(3): 61-69.
- Srinivasan K. Cumin (Cuminum cyminum) and black cumin (Nigella sativa) seeds: traditional uses, chemical constituents, and nutraceutical effects. Food Qual Saf 2018; 2(1): 1-16.
- Abd El-Hack ME, Alagawany M, Farag MR, et al. Nutritional, healthical and therapeutic efficacy of black cumin (Nigella sativa) in animals, poultry and humans. Int J Pharmacol 2016; 12(3): 232-248.
- Tekeli A. Nutritional value of black cumin (Nigella sativa) meal as an alternative proteın source in poultry nutritıon. J Anim Sci Adv 2014; 4(4): 797-806.
- Sogut B, Celik I, Tuluce Y. The effects of diet supplemented with black cumin (Nigella sativa ) upon immune potential and antioxidant marker enzymes and lipid peroxidation in broiler chicks. J Anim Vet Adv 2008; 7(10): 1196-1199.
- Kumar P, Patra AK, Mandal GP, et al. Carcass characteristics, chemical and fatty acid composition and oxidative stability of meat from broiler chickens fed black cumin (Nigella sativa) seeds. J Anim Physiol Anim Nutr (Berl) 2018; 102(3): 769-779.
- Abd El-Hack ME, Mahgoub SA, Hussein MMA, et al. Improving growth performance and health status of meat-type quail by supplementing the diet with black cumin cold-pressed oil as a natural alternative for antibiotics. Environ Sci Pollut Res Int 2018; 25(2):1157-1167.
- Kumar P, Patra AK, Mandal GP, et al. Effect of black cumin seeds on growth performance, nutrient utilization, immunity, and gut health and nitrogen excretion in broiler chickens. J Sci Food Agric 2017; 97(11): 3742-3751.
- Erener G, Altop A, Ocak N, et al. Influence of black cumin seed (Nigella sativa) and seed extract on broilers performance and total coliform bacteria count. Asian J Anim Vet Adv 2010; 5(2): 128-135.
- Shewita RS, Taha AE. Effect of dietary supplementation of different levels of black seed (Nigella Sativa) on growth performance, immunological, hematological and carcass parameters of broiler chicks. Int J Anim Vet Sci 2011; 5(5): 304-310.
- Tufan T, Arslan C, Sari M, et al. Effect of black cumin (Nigella sativa ) seeds or black cumin oil addition to japanese quail diets on growth performance, carcass traits and some blood parameters [Turkish]. Kafkas Univ Vet Fak Derg 2015; 21(4): 593-599.
- Shokrollahi B, Sharifi B. Effect of Nigella sativa seeds on growth performance, blood parameters, carcass quality and antibody production in Japanese quails. J Livest Sci 2018; 9(1): 56-64.
- Arif M, Rehman A, Abd El-Hack ME, et al. Growth, carcass traits, cecal microbial counts, and blood chemistry of meat-type quail fed diets supplemented with humic acid and black cumin seeds. Asian-Australas J Anim Sci 2018; 31(12): 1930-1938.
- Demirci M, Karsli MA, Aluç Y. Determining the effects of black cumin seed oil on performance and meat fatty acid profile of broiler chickens. S Afr J Anim Sci 2019; 49(5): 892-899.
- Singh PK, Kumar A. Effect of dietary black cumin (Nigella sativa) on the growth performance, nutrient utilization, blood biochemical profile and carcass traits in broiler chickens. Anim Nutr Feed Technol 2018; 18(3): 409-419.
- Darand M, Alavian SM, Hekmatdoost A. Nigella sativa and non-alcoholic fatty liver disease: A review of the current evidence. Hepat Mon 2018; 18(10): e68046. doi: 10.5812/hepatmon.68046.
- Awad AS, Abd Al Haleem EN, El-Bakly WM, et al. Thymoquinone alleviates nonalcoholic fatty liver disease in rats via suppression of oxidative stress, inflammation, apoptosis. Naunyn Schmiedebergs Arch Pharmacol 2016; 389(4): 381-391.
- (National Research Council). Nutrient requirements of poultry 9th ed. Washington DC, USA: National Academy Press 1994.
- AOAC International. Official method of analysis. 18th Maryland, USA: Association of analytical chemists international 2005.
- Kazak F, Cimrin T, Alaşahan S. Effects of ground fennel seed supplementation to diet on some blood and liver parameters in quails. ISPEC J Agr Sci 2020; 4(4): 1037-1052.
- Lowry OH, Rosebrough NJ, Farr AL, et al. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193(1): 265-275.
- Ohkawa H, Ohishi N, Yagi K. Assay for lipit peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95(2): 351-358.
- Ellman GL. Tissue sulphydryl groups. Arch Biochem Biophys 1959; 82(1): 70-77.
- Haag W. Zur methodik und praktischen Bedeutung der Vitamin C - Best immung be im Rind in Vergangenheit und Gegenwart [German]. Ph.D. Thesis, University of Justus Liebig, Gissen, Germany: 1985.
- Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70(1): 158-169.
- Aebi H. Catalase in vitro. Methods Enzymol 1984; 105: 121-126.
- Güvenç M, Cellat M, Gökçek İ, et al. Nobiletin attenuates acetaminophen-induced hepatorenal toxicity in rats. J Biochem Mol Toxicol 2020; 34(2): e224227. doi: 10.1002/jbt.22427.
- Al-Beitawi N, El-Ghousein SS. Effect of feeding different levels of Nigella sativa seeds (black cumin) on performance, blood constituents and carcass characteristics of broiler chicks. Int J Poult Sci 2008; 7(7): 715-721.
- Talebi A, Maham M, Asri-Rezaei S, et al. Effects of Nigella sativa on performance, blood profiles, and antibody titer against Newcastle disease in broilers. Evid Based Complement Alternat Med 2021; 2021: 2070375. doi: 10.1155/2021/2070375.
- Ghasemi HA, Kasani N, Taherpour K. Effects of black cumin seed (Nigella sativa), a probiotic, a prebiotic and a synbiotic on growth performance, immune response and blood characteristics of male broilers. Livest Sci 2014; 164: 128-134.
- Karadağoğlu O, Şahin T, Ölmez M, et al. Fatty acid composition of liver and breast meat of quails fed diets containing black cumin (Nigella sativa L.) and/or coriander (Coriandrum sativum ) seeds as unsaturated fatty acid sources. Livest Sci 2019; 223: 164-171.
- Attia YA, Al-Harthi MA. Nigella seed oil as an alternative to antibiotic growth promoters for broiler chickens. Europ Poult Sci 2015; 79: doi: 10.1399/ eps.2015.80.
- Szczerbińska D, Sulik M, Majewska D, et al. Effect of diet supplemented with black cumin (Nigella sativa) seeds on egg yolk fatty acid profile and egg yolk cholesterol level of Japanese quail (Coturnix japonica). Acta Sci Pol Zootechnica 2020; 19(3): 31-38.
- Al-Homidan A, Al-Qarawi AA, Al-Waily SA, et al. Response of broiler chicks to dietary Rhazya stricta and Nigella sativa. Br Poult Sci 2002; 43(2): 291-296.
- El-Bahr SM, Al-Ankari A, Shousha S. Immune-responsiveness, performance and blood chemistry of broiler chickens fed black cumin seed and/or turmeric. Thai J Vet Med 2021; 51(2): 267-275.
- Akhtar MS, Nasir Z, Abid AR. Effect of feeding powdered Nigella sativa seeds on poultry egg production and their suitability for human consumption. Vet Arhiv 2003; 73(3): 181-190.
- Sevim B, Çufadar Y, Bahtiyarca Y, et al. The effect of adding different levels of black cumin (Nigella sativa) seed to the feed on performance, serum parameters and reproductive hormones in male Japanese quails (Coturnix Coturnix Japonica). OKU J Inst Sci Technol 2021; 4(3): 441-451.
- Balevi T. The effects of some oils used in rations on the productivity propertions of the poultry and fatty acid compositions of poultry products [Turkish]. PhD thesis, Selcuk University. Institute of Health Sciences. Konya, Turkey: 1996.
- Shirzadegan K, Fallahpour P, Nickkhah I, et al. Black cumin (Nigella sativa) supplementation in the diet of broilers influences liver weight and its enzymes. Iran J Appl Anim Sci 2015; 5(1): 173-178.
- Aydogan I, Yildirim E, Kurum A, et al. The effect of dietary garlic (Allium sativum), black cumin (Nigella sativa) and their combination on performance, intestine morphometry, serum biochemistry and antioxidant status of broiler chickens. Braz J Poult Sci 2020; 22(4): doi. 10.1590/1806-9061-2020-1317.
- Mukai H, Noguchi T, Kamimura K, et al. Significance of elevated serum LDH (lactate dehydrogenase) activity in atopic dermatitis. J Dermatol 1990; 17(8): 477-481.
- Butt MS, Sultan MT. Nigella sativa: reduces the risk of various maladies. Crit Rev Food Sci Nutr 2010; 50(7): 654-665.
- Tülüce Y, Özkol H, Söğüt B, et al. Effects of Nigella sativa L. on lipid peroxidation and reduced glutathione levels in erythrocytes of broiler chickens. Cell Membr Free Radic Res 2009; 1(3): 95-99.
- Guler T, Ertas ON, Kizil M, et al. Effect of dietary supple-mental black cumin seeds on antioxidant activity in broilers. Medycyna Wet 2007; 63(9):1060-1063.
- Hassan SS. Evaluation of Nigella sativa seeds on broiler chicks hematological, blood biochemical parameters and antioxidant enzymes. Egypt Poult Sci 2021; 41(3): 439-459.
- Brody T. Nutritional biochemistry. 2nd San Diego, USA: Academic Press 1999; 157-262.
- Attia A, Ragheb A, Sylwestrowicz T, et al. Attenuation of high cholesterol-induced oxidative stress in rabbit liver by thymoquinone. Eur J Gastroenterol Hepatol 2010; 22(7): 826-834.
- Türkdoğan MK, Ozbek H, Yener Z, et al. The role of Urtica dioica and Nigella sativa in the prevention of carbon tetrachloride-induced hepatotoxicity in rats. Phytother Res 2003; 17(8): 942-946.
- Güzelsoy P, Aydin S, Basaran N. Potential effects of thymoquinone the active constituent of black seed (Nigella sativa) on human health. J Lit Pharm Sci 2018; 7(2): 118-135.
- Yüncü M, Şahin M, Bayat N, et al. Effects of Nigella sativa oil on rat liver development [Turkish]. Gaziantep Med J 2013; 19(3): 180-184.