Document Type : Original Article
Authors
1 Veterinary Surgeon, FourVet Veterinary Clinic, Kayseri, Türkiye
2 Department of Surgery, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Türkiye
3 Department of Pharmaceutical Basic Science, Faculty of Pharmacy, University of Erciyes, Kayseri, Türkiye
Abstract
In the present study the effect of ozone therapy on hydrofluoric acid (HFA) related eye burn was investigated in rats. A Total 20 healthy male Wistar albino rats (weighing 250 - 300 g with the age of 16 weeks) were used. They were divided into groups (experimental and control groups) of 10 rats being housed individually and fed ad libitum. The HFA (2.00%) burn was created in all animals. The ozonized (20.00 µg O3 mL-1) bi-distilled water was applied as a drop (10.00 µL each drop) every 8 hr for 7 days in the experimental group. At the same time, 0.90% NaCl was applied as drop (10.00 µL each drop) every 8 hr for 7 days in the control group. In the experimental group, intensive inflammation, angiogenesis, epithelial damage and stromal edema were detected in one animal. Epithelial vascularization and stromal edema were seen in four animals. In control group, only two animals’ corneal structures were normal. Inflammation, angiogenesis, epithelial damage, fibrosis, epithelial vascularization and stromal edema were detected in the rest. As a result of this study, it was observed that local usage of ozone therapy had a positive effect on the healing of corneal burns caused by HFA. It was concluded that more ozone-related studies should be done to enlighten the subject.
Keywords
- Müftüoğlu IK, Akova YA, Çetinkaya A. Clinical spectrum and treatment approaches in corneal burns [Turkish]. Turk J Opthalmol 2015; 45(5): 182-187.
- Altan S, Oğurtan Z. Dimethyl sulfoxide but not indomethacin is efficient for healing in hydrofluoric acid eye burns. Burns 2017; 43(1): 232-244.
- Spöler F, Frentz M, Först M, et al. Analysis of hydrofluoric acid penetration and decontamination of the eye by means of time-resolved optical coherence tomography. Burns 2008; 34(4): 549-555.
- Liu X. Effect of ozone therapy on cell apoptosis and angiogenesis in retina tissue of diabetic retinopathy rats. J Hainan Med Univ. 2016; 22(10): 1-4.
- Altınbilek T, Kaya E, Uyar M, et al. Ozone therapy for treatment of wounds [Turkish] Interg Tıp Derg 2014; 2(2): 44-48.
- Kaya A, Sonmez M, Kar T, et al. Efficiency of ozone therapy in a rat model of experimental uveitis. Ocul Immunol Inflamm 2017; 25(5): 695-700.
- Spadea L, Tonti E, Spaterna A, et al. Use of ozone-based eye drops: a series of cases in veterinary and human spontaneous ocular pathologies. Case Rep Ophthalmol 2018; 9(2): 287-298.
- Hatipoğlu F, Ogurtan Z, Sezer AD, et al. Effect of laminarin and chitosan gel formulations on the treatment of hydrofluoric acid induced corneal burns in the rabbits. Revue Méd Vét 2008; 159(4): 207-214.
- Lee CM, Jung WK, Na G, et al. Inhibitory effects of the platelet-activating factor receptor antagonists, CV-3988 and Ginkgolide B, on alkali burn-induced corneal neovascularization. Cutan Ocul Toxicol 2015; 34(1): 53-60.
- Sancak IG, Bozkurt MF. Healing effect of cornea conjunctival transposition (CCT) technique on corneal healing in New Zealand rabbits (Oryctolagus cuniculus) [Turkish]. Ankara Üniv Vet Fak Derg 2010; 57(4): 235-240.
- Singh P, Tyagi M, Kumar Y, et al. Ocular chemical injuries and their management. Oman J Ophthalmol 2013; 6(2): 83-86.
- Moghadam MH, Jafarinasab MR, Yousefi Z, et al. Aloe vera gel-derived eye drops for alkaline corneal injury in a rabbit model. J Ophthalmic Vis Res 2020; 15(1): 7-15.
- Varol K, Koç AN, Çakır Bayram L, et al. Studies on the effectiveness of ozone therapy on the treatment of experimentally induced keratitis with Candida albicans in rabbits. Semin Ophthalmol 2022; 37(2): 253-264.
- Ollivier FJ. Bacterial corneal diseases in dogs and cats. Clin Tech Small Anim Pract 2003; 18(3):193-198.
- Lorenzo-Martin E, Gallego-Muñoz P, Mar S, et al. Dynamic changes of the extracellular matrix during corneal wound healing. Exp Eye Res 2019; 186: 107704. doi: 10.1016/j.exer.2019.107704.
- Yi Q, Zou WJ. The wound healing effect of doxycycline after corneal alkali burn in rats. J Ophthamol 2019; 5168652. doi: 10.1155/2019/5168652.
- Kim EC, Kim TK, Park SH, et al. The wound healing effects of vitamin A eye drops after a corneal alkali burn in rats. Acta Ophthalmolog 2012; 90(7): e540-e546.
- Hoffart L, Matonti F, Conrath J, et al. Inhibition of corneal neovascularization after alkali burn: comparison of the different doses of bevacizumab in monotherapy or associated with dexamethasone. Clin Exp Opthalmol 2010; 38(4): 346-352.
- Kirkpatrick JJ, Enion DS, Burd DA. Hydrofluoric acid burns: a review. Burns 1995; 21(7): 483-493.
- Uysal B, Demirbag S, Poyrazoglu Y, et al. Medical ozone therapy decreases postoperative uterine adhesion formation in rats. Arch Gynecol Obstet 2012; 286(5): 1201-1207.
- Yamanel L, Kaldirim U, Oztas Y, et al. Ozone therapy and hyperbaric oxygen treatment in lung injury in septic rats. Int J Med Sci 2011; 8(1): 48-55.
- Oğurtan Z, Hatipoğlu F, Ceylan C, et al. Treatment of corneal hydrofluoric acid burns in rabbits. Revue Méd Vét 2002; 153(4): 269-74.
- Barrientez B, Nicholas SE, Whelchel A, et al. Corneal injury: clinical and molecular aspects. Exp Eye Res 2019; 186: 107709. doi: 10.1016/j.exer.2019.107709.
- Rigas B, Huang W, Honkanen R. NSAID-induced corneal melt: clinical importance, pathogenesis, and risk mitigation. Surv Ophthalmol 2020; 65(1): 1-11.
- Sağsöz H, Topaloğlu U, Güney Saruhan B, et al. Distribution of CD8 and CD68 positive cells in acid corneal burns in rabbit [Turkish]. Dicle Üniv Vet Fak Derg 2018; 11(1): 22-28.
- Schrage NF, Langefeld S, Zschocke J, et al. Eye burns: an emergency and continuing problem. Burns 2000; 26(8): 689-699.
- Gerard M, Merle H, Chiambaretta F, et al. An amphoteric rinse used in the emergency treatment of serious ocular burn. Burns 2002; 28(7): 670-673.
- Varol K. Determination of maximum ozone concentrations and half-life of ozone in some drinking water and high quality pure water produced in new system devices. Fresenius Environ Bull 2022; 31(2): 2285-2294.
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