Veterinary Research Forum
Vet Res Forum

Effects of ozone therapy on acidic corneal burns in rats

Document Type : Original Article

Authors

Seren Aydoğan 1
Hanifi Erol 2
Münevver Baran 3

1 Veterinary Surgeon, FourVet Veterinary Clinic, Kayseri, Türkiye

2 Department of Surgery, Faculty of Veterinary Medicine, University of Erciyes, Kayseri, Türkiye

3 Department of Pharmaceutical Basic Science, Faculty of Pharmacy, University of Erciyes, Kayseri, Türkiye

https://doi.org/10.30466/vrf.2022.551199.3432
Abstract
In the present study the effect of ozone therapy on hydrofluoric acid (HFA) related eye burn was investigated in rats. A Total 20 healthy male Wistar albino rats (weighing 250 - 300 g with the age of 16 weeks) were used. They were divided into groups (experimental and control groups) of 10 rats being housed individually and fed ad libitum. The HFA (2.00%) burn was created in all animals. The ozonized (20.00 µg O3 mL-1) bi-distilled water was applied as a drop (10.00 µL each drop) every 8 hr for 7 days in the experimental group. At the same time, 0.90% NaCl was applied as drop (10.00 µL each drop) every 8 hr for 7 days in the control group. In the experimental group, intensive inflammation, angiogenesis, epithelial damage and stromal edema were detected in one animal. Epithelial vascularization and stromal edema were seen in four animals. In control group, only two animals’ corneal structures were normal. Inflammation, angiogenesis, epithelial damage, fibrosis, epithelial vascularization and stromal edema were detected in the rest. As a result of this study, it was observed that local usage of ozone therapy had a positive effect on the healing of corneal burns caused by HFA. It was concluded that more ozone-related studies should be done to enlighten the subject.     

Keywords

Burn
Cornea
Hydrofluoric acid
Ozone
Rat
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Veterinary Research Forum
Volume 14, Issue 4
April 2023
Pages 195-199

  • Receive Date 31 March 2022
  • Revise Date 13 June 2022
  • Accept Date 29 August 2022

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