Veterinary Research Forum
Vet Res Forum

Immunohistochemical determination of somatostatin release in gastric tissue of rats fed with a high-fat and cholesterol diet

Document Type : Original Article

Authors

Habibe Gündoğdu 1
Ebru Karadağ Sari 2

1 Department of Histology and Embryology, Faculty of Medicine, Ataturk University, Erzurum, Türkiye

2 Department of Histology and Embryology, Faculty of Veterinary Medicine, Kafkas University, Kars, Türkiye

https://doi.org/10.30466/vrf.2022.551598.3441
Abstract
This study aimed to investigate the effects of a high-fat and cholesterol diet (HFCD) on rats gastric mucosa. In the study, a total of 16 (40-day-old Sprague Dawley) male rats were used and randomly divided into two groups (each consisted of eight rats). Rats in the control group had no implementations other than normal feeding. For 10 weeks, rats in a high-fat with cholesterol diet group had daily energy amounts provided by pellet feed mixed with 65.00% butter and 2.00% cholesterol. Before beginning the study and at the end, rats live weight was recorded and their blood samples were taken for biochemical analyses. Hematoxylin and Eosin and Crossman’s triple staining techniques were used to investigate the general structure of gastric tissue. Rats fed with HFCD had statistically significant increases in live weight and total cholesterol values, and were identified to have gastric tissue degeneration. The rats gastric tissue in control group had more intense somatostatin (SST) immunoreactivity in parietal and chief cells than the HFCD group. It was determined that feeding with the HFCD has a negative effect on SST secretion in rats and hence, this may have important areas of use such as in gastric cancer treatment and preventing complications linked to gastric diseases.

Keywords

Fat
Cholesterol
Rat
Somatostatin
Stomach
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Veterinary Research Forum
Volume 14, Issue 6
June 2023
Pages 309-315

  • Receive Date 08 April 2022
  • Revise Date 14 June 2022
  • Accept Date 01 August 2022

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