Document Type : Short Communication
Authors
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Ataturk University, Erzurum, Türkiye
Abstract
The aim of this study was to determine the prevalence of the Parainfluenza 3 (PI3) virus antigen with histopathological and immunohistochemical (IHC) staining methods in sheep lung samples collected at slaughter in Erzurum province. Between August and November 2017, 1462 sheep were dissected in the slaughterhouse, and their lungs were examined macroscopically. In total, 100 of the lung samples with pneumonia were selected. Routine histopathological and immunohistochemical staining of the collected lung tissues with pneumonia were performed. Pneumonia observed through macroscopic and histopathological examination of the lung samples was classified as purulent catarrhal bronchopneumonia (14%), fibrinous bronchopneumonia (23%), interstitial pneumonia (69%), granulomatous pneumonia (7%), verminous pneumonia (19%), and pulmonary adenomatous (6%) (n = 100). Two or three types of pneumonia were observed in many of the same cases. The PI3 virus antigen positivity rate in the IHC staining of the sheep lung samples was 19%. In the IHC method, positive samples were found mostly in the alveolar macrophages and in the cytoplasm of the bronchial, bronchiolar, and alveolar epithelial cells. As a result of the research findings, the prevalence of the PI3 virus in sheep in Erzurum province was determined to be 19% by immunohistochemical method.
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