Veterinary Research Forum
Vet Res Forum

Phylogenetic analysis of pigeon adenovirus 1 in clinical specimens of domestic pigeons (Columba livia domestica) in Iran

Document Type : Original Article

Authors

Elham Rahimi Sardo 1
Forough Talazadeh 1
Ramezan Ali Jafari 1
Masoud Reza Seyfi 2

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

https://doi.org/10.30466/vrf.2022.553719.3471
Abstract
An internationally identified syndrome that leads to deaths between domestic and ornamental pigeons, particularly after racing is young pigeon disease syndrome (YPDS). This study was conducted to determine the status of pigeon adenoviral infection and molecularly characterize the pigeon adenovirus in Ahvaz pigeons. Sixty stool samples of healthy pigeons (young pigeons and adult pigeons) and 60 stool samples of diseased pigeons (young and adults) with symptoms of lethargy, weight loss, crop stasis, vomiting and diarrhea were examined. Samples were screened for aviadenoviruses by polymerase chain reaction (PCR) assay and degenerated primers set to target the aviadenovirus polymerase (pol) gene were used which was designed in this study. Screening for pigeon adenovirus 1 (PiAdV-1) was performed using a primer pair that targeted the fiber gene of PiAdV-1. Out of 120 stool samples, six samples (5.00%) were positive for aviadenovirus. The results showed that independent from pigeons’ age status, 5.00 and 3.33% of sick and of healthy pigeons were positive for PiAdV-1, respectively. Genomic sequencing revealed that the viruses detected in Ahvaz pigeons belonged to the PiAdV-1 genotype. The results in pigeons revealed a 98.10 - 99.53% nucleotide similarity when compared to other strains of PiAdV-1 (TR/SKPA20, P18-05523-6 and strain IDA4) formerly deposited in GenBank® in Türkiye, Australia and The Netherlands. As far as the authors know, this was the first record of phylogenetic analysis of PiAdV-1 in Iran.

Keywords

Adenoviral infections
Molecular identification
Pigeon
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Veterinary Research Forum
Volume 14, Issue 6
June 2023
Pages 329-334

  • Receive Date 13 May 2022
  • Revise Date 24 August 2022
  • Accept Date 18 October 2022

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