Document Type : Original Article
Department of Anatomy, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Türkiye
Department of Biology, Faculty of Science and Art, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye
Department of Medical Services and Techniques, Isparta Health Services Vocational School, Süleyman Demirel University, Isparta, Türkiye
Department of Biochemistry, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Türkiye
Organisms have evolved defense mechanisms to protect themselves from stressful conditions. The expression of heat shock proteins is considered a valid indication of protection from the adverse effects of hostile conditions. In this study, we used immunohistochemistry to investigate the seasonal effects of some abiotic factors on heat shock protein 70 and 90 (HSP70 and HSP90) expression in the liver, gills, and muscle tissues of 24 Common carp (Cyprinus carpio) caught in Karataş Lake (Burdur, Türkiye) using gillnets of various mesh sizes. We also measured some physicochemical parameters on-site at sampling time and took water samples for further analyses of other physicochemical parameters and heavy metals. Immunostaining for HSP90 was stronger than for HSP70 in both liver and gill samples. Liver and gill structures exhibited significant seasonal differences in HSP70 and HSP90 immunoreactivity, and the same was true for immunostaining for HSP70 and HSP90 in muscle samples. Some physicochemical properties seemed to vary considerably between seasons, with Fe, Mn, and Zn levels tending to exhibit changes throughout the seasons. However, these levels were considered acceptable for human health. In conclusion, this study suggests that substantial changes in HSP70 and HSP90 expression may be essential for seasonal adaptation and tolerance. Further research on fish HSPs would greatly contribute to aquaculture, which is essential for meeting food requirements.
- López-Maury L, Marguerat S, Bähler J. Tuning gene expression to changing environments: from rapid responses to evolutionary adaptation. Nat Rev Genet 2008; 9(8): 583-593.
- Jesus TF, Inácio A, Coelho MM. Different levels of hsp70 and hsc70 mRNA expression in Iberian fish exposed to distinct river conditions. Genet Mol Biol 2013; 36(1): 61-69.
- Lindquist S. The heat-shock response. Annu Rev Biochem 1986; 55: 1151-1191.
- Ming J, Xie J, Xu P, et al. Molecular cloning and expression of two HSP70 genes in the Wuchang bream (Megalobrama amblycephala Yih). Fish Shellfish Immunol 2010; 28(3): 407-418.
- Su X, Du L, Li Y, et al. Cloning and expression of HSP70 gene of sipuncula Phascolosoma esculenta. Fish Shellfish Immunol 2010; 28(3): 461-466.
- Liu HH, He JY, Chi CF, et al. Differential HSP70 expression in Mytilus coruscus under various stressors. Gene 2014; 543(1): 166-173.
- Franzellitti S, Fabbri E. Differential HSP70 gene expression in the Mediterranean mussel exposed to various stressors. Biochem Biophys Res Commun 2005; 336(4): 1157-1163.
- Liu T, Pan L, Cai Y, et al. Molecular cloning and sequence analysis of heat shock proteins 70 (HSP70) and 90 (HSP90) and their expression analysis when exposed to benzo(a)pyrene in the clam Ruditapes philippinarum. Gene 2015; 555(2): 108-118.
- Erbse A, Mayer MP, Bukau B. Mechanism of substrate recognition by Hsp70 chaperones. Biochem Soc Trans 2004; 32(Pt 4): 617-621.
- Chen B, Piel WH, Gui L, et al. The HSP90 family of genes in the human genome: insights into their divergence and evolution. Genomics 2005; 86(6): 627-637.
- Chen B, Zhong D, Monteiro A. Comparative genomics and evolution of the HSP90 family of genes across all kingdoms of organisms. BMC Genomics 2006; 7: 156. doi: 10.1186/1471-2164-7-156.
- Sreedhar AS, Kalmár E, Csermely P, et al. Hsp90 isoforms: functions, expression and clinical importance. FEBS Lett 2004; 562(1-3): 11-15.
- Tine M, Bonhomme F, Mckenzie DJ, et al. Differential expression of the heat shock protein Hsp70 in natural populations of the tilapia, Sarotherodon melanotheron, acclimatized to a range of environmental salinities. BMC Ecol 2010; 10: 11. doi: 10.1186/1472-6785-10-11.
- Purohit GK, Mahanty A, Suar M, et al. Investigating hsp gene expression in liver of Channa striatus under heat stress for understanding the upper thermal acclimation. Biomed Res Int 2014; 2014: 381719. doi: 10.1155/2014/381719.
- Peng G, Zhao W, Shi Z, et al. Cloning HSP70 and HSP90 genes of kaluga (Huso dauricus) and the effects of temperature and salinity stress on their gene expression. Cell Stress Chaperones 2016; 21(2): 349-359.
- Ma F, Luo L. Genome-wide identification of Hsp70/110 genes in rainbow trout and their regulated expression in response to heat stress. PeerJ 2020; 8: e10022. doi: 10.7717/peerj.10022.
- Wang J, Wei Y, Li X, et al. The identification of heat shock protein genes in goldfish (Carassius auratus) and their expression in a complex environment in Gaobeidian Lake, Beijing, China. Comp Biochem Physiol C Toxicol Pharmacol 2007; 145(3): 350-362.
- Padmini E, Usha Rani M. Impact of seasonal variation on HSP70 expression quantitated in stressed fish hepatocytes. Comp Biochem Physiol B Biochem Mol Biol 2008; 151(3): 278-285.
- Bildik A, Asıcı Ekren GS, Akdeniz G, et al. Effect of environmental temperature on heat shock proteins (HSP30, HSP70, HSP90) and IGF-I mRNA expression in Sparus aurata. Iran J Fish Sci 2019; 18(4): 1014-1024.
- Colín-García NA, Chiappa-Carrara X, Campos JE, et al. Differential gene expression of heat shock protein in response to thermal stress, in two Fundulus species endemic to the Gulf of Mexico. Rev Mex Biodiv 2019; 90: e902663. doi: 10.22201/ib.20078706e.2019. 2663.
- Macêdo AKS, Santos KPED, Brighenti LS, et al. Histological and molecular changes in gill and liver of fish (Astyanax lacustris Lütken, 1875) exposed to water from the Doce basin after the rupture of a mining tailings dam in Mariana, MG, Brazil. Sci Total Environ 2020; 735: 139505. doi: 10.1016/j.scitotenv.2020. 139505.
- Kir İ, Tekin-Özcan S, Barlas M. Heavy metal concentrations in organs of Rudd, Scardinus erythrophthalmus, 1758 populating Lake Karataş-Turkey. Fresenius Environ Bull 2006; 15(1): 25-29.
- Çetin B. The use of wetland of Karataş (Bahçeözü) Lake (Burdur-Karamanlı) and a geographical look at the emerging problems [Turkish]. Nat Sci 2009; 4(4): 157-174.
- Wallace DR. Environmental pesticides and heavy metals - Role in breast cancer. In: Larramendy ML, Soloneski S (Eds). Toxicity and hazard of agrochemicals. London, United Kingdom: IntechOpen 2015; 39-70.
- Alengebawy A, Abdelkhalek ST, Qureshi SR, et al. Heavy metals and pesticides toxicity in agricultural soil and plants: ecological risks and human health implications. Toxics 2021; 9(3): 42. doi: 10.3390/toxics9030042.
- Piano A, Valbonesi P, Fabbri E. Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissues of Ostrea edulis exposed to heat and heavy metals. Cell Stress Chaperones 2004; 9(2): 134-142.
- Gibson RN. The use of anaesthetic quinaldine in fish ecology. J Anim Ecol 1967; 36(2): 295-301.
- Öztop M, Cinar K, Turk S. Immunolocalization of natriuretic peptides and their receptors in goat (Capra hircus) heart. Biotech Histochem 2018; 93(6): 389-404.
- Kaptan H, Tekin-Özan S. Determination of the heavy metals levels in some tissues and organs of carp (Cyprinus carpio, 1758) living in water, sediment of Eğirdir Lake [Turkish]. SDU J Sci 2014; 9(2): 44-60.
- Guidelines for drinking water quality. 4th ed. Geneva, Switzerland: World Health Organization 2017.
- De Smet H, Blust R. Stress response and changes in protein metabolism in carp Cyprinus carpio during cadmium exposure. Ecotoxicol Environ Saf 2001; 48(3): 255-262.
- Thophon S, Kruatrachue M, Upatham ES, et al. Histopathological alterations of white seabass, Lates calcarifer, in acute and subchronic cadmium exposure. Environ Pollut 2003; 121(3): 307-320.
- Yoo JL, Janz DM. Tissue-specific HSP70 levels and reproductive physiological responses in fishes inhabiting a metal-contaminated creek. Arch Environ Contam Toxicol 2003; 45(1): 110-120.
- Williams JH, Petersen NS, Young PA, et al. Accumulation of Hsp70 in juvenile and adult rainbow trout gill exposed to metal-contaminated water and/or diet. Environ Toxicol Chem 1996; 15(8): 1324-1328.
- Rajeshkumar S, Munuswamy N. Impact of metals on histopathology and expression of HSP70 in different tissues of Milkfish (Chanos chanos) of Kaattuppalli Island, South East Coast, India. Chemosphere 2011; 83(4): 415-421.
- Safari R, Shabani A, Ramezanpour S, et al. Alternations of heat shock proteins (Hsp70) gene expression in liver and gill of Persian sturgeon (Acipenser persicus Borodin, 1987) exposed to cadmium chloride. Iran J Fish Sci 2014; 13(4): 979-997.
- Nair M, Jayalakshmy KV, Balachandran KK, et al. Bioaccumulation of toxic metals by fish in a semi-enclosed tropical ecosystem. Environ Forensics 2006; 7(3): 197-206.
- Hong Z, Zhao Q, Chang J, et al. Evaluation of water quality and heavy metals in wetlands along the Yellow River in Henan Province. Sustainability 2020; 12(4): 1300. doi: 10.3390/su12041300.
- Guo W, Zou J, Liu S, et al. Seasonal and spatial variation in dissolved heavy metals in Liaodong Bay, China. Int J Environ Res Public Health 2022; 19(1): 608. doi: 10.3390/ijerph19010608.
- Alsherif EA, Al-Shaikh TM, AbdElgawad H. Heavy metal effects on biodiversity and stress responses of plants inhabiting contaminated soil in Khulais, Saudi Arabia. Biology (Basel) 2022; 11(2): 164. doi: 10.3390/ biology11020164.
- Das P, Gupta A, Manna SK. Heat shock protein 70 expression in different tissues of Cirrhinus mrigala (Ham.) following heat stress. Aquac Res 2005; 36(6): 525-529.
- Camargo MMP, Martinez CBR. Histopathology of gills, kidney and liver of a Neotropical fish caged in an urban stream. Neotrop Ichthyol 2007; 5(3): 327-336.
- Deane EE, Woo NY. Differential gene expression associated with euryhalinity in sea bream (Sparus sarba). Am J Physiol Regul Integr Comp Physiol 2004; 287(5): R1054-R1063.
- Shahjahan M, Khatun MS, Mun MM, et al. Nuclear and cellular abnormalities of erythrocytes in response to thermal stress in common carp Cyprinus carpio. Front Physiol 2020; 11: 543. doi: 10.3389/fphys.2020.00543.