Veterinary Research Forum
Vet Res Forum

Seasonal variation in the expression pattern of heat shock protein 70 and 90 in Common carp (Cyprinus carpio) from Karataş Lake, Burdur, Türkiye

Document Type : Original Article

Authors

Nilgün Kuru 1
Mustafa Öztop 2
Emel Demirbağ 3
Nazlı Ercan 4

1 Department of Anatomy, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Türkiye

2 Department of Biology, Faculty of Science and Art, Burdur Mehmet Akif Ersoy University, Burdur, Türkiye

3 Department of Medical Services and Techniques, Isparta Health Services Vocational School, Süleyman Demirel University, Isparta, Türkiye

4 Department of Biochemistry, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Türkiye

https://doi.org/10.30466/vrf.2022.558983.3568
Abstract
Organisms have evolved defense mechanisms to protect themselves from stressful conditions. The expression of heat shock proteins is considered a valid indication of protection from the adverse effects of hostile conditions. In this study, we used immunohistochemistry to investigate the seasonal effects of some abiotic factors on heat shock protein 70 and 90 (HSP70 and HSP90) expression in the liver, gills, and muscle tissues of 24 Common carp (Cyprinus carpio) caught in Karataş Lake (Burdur, Türkiye) using gillnets of various mesh sizes. We also measured some physicochemical parameters on-site at sampling time and took water samples for further analyses of other physicochemical parameters and heavy metals. Immunostaining for HSP90 was stronger than for HSP70 in both liver and gill samples. Liver and gill structures exhibited significant seasonal differences in HSP70 and HSP90 immunoreactivity, and the same was true for immunostaining for HSP70 and HSP90 in muscle samples. Some physicochemical properties seemed to vary considerably between seasons, with Fe, Mn, and Zn levels tending to exhibit changes throughout the seasons. However, these levels were considered acceptable for human health. In conclusion, this study suggests that substantial changes in HSP70 and HSP90 expression may be essential for seasonal adaptation and tolerance. Further research on fish HSPs would greatly contribute to aquaculture, which is essential for meeting food requirements.

Keywords

Common carp
Gill
Heavy metal
Liver
Muscle

Subjects

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Veterinary Research Forum
Volume 14, Issue 9
September 2023
Pages 471-479

  • Receive Date 27 July 2022
  • Revise Date 16 December 2022
  • Accept Date 17 December 2022

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