Veterinary Research Forum
Vet Res Forum

Plasma microRNAs as non-invasive biomarkers in bovine endometritis caused by Gram-negative and Gram-positive bacteria

Document Type : Original Article

Authors

Arman Shokri 1
Reza Asadpour 1
Razi Jafai-joozani 1
Esmaeil Babaei 2
Abolfazl Hajibemani 1
Gholamreza Hamidian 3

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

2 Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran

3 Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran

https://doi.org/10.30466/vrf.2022.555375.3505
Abstract
The purpose was to identify differentially expressed plasma microRNAs (miRNAs) in cows with clinical and subclinical endometritis. In this study clinical endometritis (CE; n = 23) based on vaginal discharge score (VDS), subclinical endometritis (SCE; n = 17) based on VDS (0), and endometrial cytology (the presence of 8.00% polymorphonuclear neutrophils (PMN) on days 21-31 and 5.00% on days 41-51 days in milk (DIM) and healthy cows (n = 21) based on vaginal discharge score (0), and endometrial cytology (< 5.00% PMN on days 21 - 31 and < 5.00% on days 41 - 51 DIM) were selected. The results showed that the expression level of miR-146a was significantly higher in the CE (19.17-fold), and SCE (6.22-fold) groups than those of healthy cows. The relative transcript abundance of miR-223 was considerably down-regulated in the CE (0.26-fold) and SCE (0.06-fold) compared to the healthy cows. The expression levels of miR-146a and miR-223 were significantly higher in the CE group which could be caused by Gram-negative bacterial infection. Our results showed that the expression level of plasma miRNAs postpartum could be used as a reliable marker to distinguish between SCE, CE and healthy cows.

Keywords

Bacteria
Dairy cattle
Endometritis
microRNAs
Plasma

Subjects

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Veterinary Research Forum
Volume 14, Issue 8
August 2023
Pages 437-445

  • Receive Date 07 June 2022
  • Accept Date 01 August 2022

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