Veterinary Research Forum
Vet Res Forum

How origin of ovaries influences the vitrification outcome of bovine ovarian tissue: effects of side of ovaries and corpus luteum

Document Type : Short Communication

Authors

Mohammadhamed Shahsavari 1
Gholamali Moghaddam 1
Kele Amaral Alves 2
Benner Geraldo alves 3
Laritza Ferreira de Lima 3
Golshan Azimi 4
Deysi jouana Dipaz Berrocal 3
Lucina Mascena Silva 3
Yago Pinto da Silva 3
Diego Alberto Montano Vizcarra 3
José Richardo de Figuereido 3
Ana Paula Ribeiro Rodrigues 3

1 Department of Animal Sciences, Faculty of Agriculture, University of Tabriz, Tabriz, Iran

2 Department of Gynecology and Obstetrics, Medicine College, University of São Paulo, Ribeirão Preto, Brazil

3 Department of Basic Science, Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles, State University of Ceara, Fortaleza, Brazil

4 Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran

https://doi.org/10.30466/vrf.2023.1972637.3684
Abstract
Although cryopreservation of ovarian tissue has advanced greatly, it remains a challenge, and protocols should be optimized to handle the heterogeneous nature of ovarian samples. In an effort to address this factor, the present study evaluated the effects of corpus luteum (CL) and side of ovaries (right versus left) on cellular morphology and viability of vitrified bovine ovarian fragments in a closed system. The ovaries were categorized according to whether they had a CL and which side they were on, and then divided into six groups: 1) CL+ (with CL) group; 2) CL˗ (without CL) group; 3) right ovaries group; 4) left ovaries group; 5) fresh control group (ovaries without vitrification or culture that were not selected for CL or ovarian side) and 6) In vitro culture medium control group (non-vitrified ovaries that were not selected for the presence or absence of CL or side of the ovaries). The current study shows that the CL˗ and right groups had the greatest percentage of follicles with normal morphology compared to other vitrified-warmed groups. Furthermore, the levels of necrosis and tissue damage of the right cultured group were the lowest compared to other groups. It was shown that bovine ovarian tissues derived from right ovaries and ovaries without a corpus luteum can be functionally and morphologically preserved after vitrification. For the first time, the present study suggests that bovine ovarian tissue vitrification can be improved by considering the origin of the ovaries.

Keywords

Corpus luteum
Cryopreservation
Immunolocalization
Ovarian tissue
Vitrification

Subjects

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Veterinary Research Forum
Volume 14, Issue 12
December 2023
Pages 673-679

  • Receive Date 14 December 2022
  • Revise Date 14 February 2023
  • Accept Date 08 April 2023

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