Document Type : Original Article

Authors

1 Hebei university of engineering

2 Department for safety supervision of animal products,China Animal Health and Epid-emiology Center, Qingdao, P. R. China.

3 Faculty of Agriculture, Forestry and Food Engineering, Yibin University; Yibin Key Laboratory of Zoological Diversity and Ecological Conservation, Yibin, P.

4 College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, Hebei, P. R. China.

Abstract

Feline calicivirus (FCV) is a highly contagious pathogen seriously affecting the upper respiratory tract and producing oral diseases in the feline. Despite widespread vaccination, the prevalence of FCV remains high. In this study, the FCV qd/2019/china was isolated from a domestic feline oropharyngeal swab collected from Qingdao, China. The virus was purified using the plaque assay and identified using the PCR and IFA methods, the capsid amino acid, VP1 of qd/2019/china showed sequence identity with the other isolates ranging between 83.9% (ym3/2001/jp) to 91.1% (CH-JL4). The sequence of the capsid amino acid revealed qd/2019/china to be closely related to CH-JL4 and clustered with CH-JL4 in the phylogenetic tree. The phylogenetic analysis indicated that the complete genome (GenBank accession No. MZ322896) of qd/2019/china and CH-JL4 was also classified into the same cluster. While the recombination analysis with Simplot indicated that the qd/2019/china originated from the recombination of CH-JL4 and HRB-SS, and the region 3821–5301 nt originated from HRB-SS. Further, the region 3821–5301 nt were found to belong to the protease-polymerase (PP) of HRB-SS. Here, we isolated a new recombinant virus, FCV qd/2019/china. Therefore, these results would be beneficial for understanding the evolution of FCV.

Keywords

Main Subjects