Veterinary Research Forum
Vet Res Forum

Isolation and molecular characteristics of a recombinant feline calicivirus from Qingdao, China

Document Type : Original Article

Authors

Yongxiang Liu 1
Lide Qin 2
Xiaoliang Hu 3
Yanmei Jiang 1

1 Department of Veterinary Medicine, College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, China

2 Department for Safety Supervision of Animal Products, China Animal Health and Epidemiology Center, Qingdao, China

3 Department of Veterinary Medicine, Faculty of Agriculture, Forestry and Food Engineering, Yibin University, Yibin, China

https://doi.org/10.30466/vrf.2023.1975795.3701
Abstract
Feline calicivirus (FCV) is a highly contagious pathogen seriously affecting the upper respiratory tract and producing oral diseases in the feline. Despite widespread vaccination, the prevalence of FCV remains high. In this study, the FCV qingdao (qd)/2019/china was isolated from a domestic feline oropharyngeal swab collected from Qingdao, China. The virus was purified using the plaque assay and identified using the Polymerase chain reaction and indirect immunofluorescence assay methods, the capsid amino acid, VP1 of qd/2019/china, showed sequence identity with the other isolates ranging between 83.90% (ym3/2001/jp) and 91.10% (CH-JL4). The sequence of the capsid amino acid revealed qd/2019/china to be closely related to CH-JL4 and clustered with CH-JL4 in the phylogenetic tree. The phylo-genetic analysis indicated that the complete genomes (GenBank® accession No. MZ322896) of qd/2019/china and CH-JL4 were also classified into the same cluster. The recombination analysis with Simplot indicated that the qd/2019/china originated from the recombination of CH-JL4 and HRB-SS, and the region 3,821 - 5,301 nt originated from HRB-SS. Further, the region 3,821 - 5,301 nt were found to belong to the protease-polymerase (PP) of HRB-SS. Here, we isolated a new recombinant virus, FCV qd/2019/china. Therefore, these results would be beneficial for better understanding of the evolution and epidemiology of FCV.

Keywords

Feline calicivirus
Isolation
Recombination

Subjects

  1. Tian J, Liu D, Liu Y, et al. Molecular characterization of a feline calicivirus isolated from tiger and its patho-genesis in cats. Vet Microbiol 2016; 192: 110-117.
  2. Evermann JF, Heeney JL, Roelke ME, et al. Biological and pathological consequences of feline infectious peritonitis virus infection in the cheetah. Arch Virol 1988; 102(3-4): 155-171.
  3. Kadoi K, Kiryu M, Iwabuchi M, et al. A strain of calicivirus isolated from lions with vesicular lesions on tongue and snout. New Microbiol 1997; 20(2): 141-148.
  4. Martella V, Pratelli A, Gentile M, et al. Analysis of the capsid protein gene of a feline-like calicivirus isolated from a dog. Vet Microbiol 2002; 85(4): 315-322.
  5. Di Martino B, Di Rocco C, Ceci C, et al. Characterization of a strain of feline calicivirus isolated from a dog faecal sample. Vet Microbiol 2009; 139(1-2): 52-57.
  6. Martella V, Pinto P, Lorusso E, et al. Detection and full-length genome characterization of novel canine vesiviruses. Emerg Infect Dis 2015; 21(8): 1433-1436.
  7. Battilani M, Vaccari F, Carelle MS, et al. Virulent feline calicivirus disease in a shelter in Italy: a case description. Res Vet Sci 2013; 95(1): 283-290.
  8. Rohayem J, Bergmann M, Gebhardt J, et al. Antiviral strategies to control calicivirus infections. Antiviral Res 2010; 87(2): 162-178.
  9. Fino VR, Kniel KE. UV light inactivation of hepatitis A virus, Aichi virus, and feline calicivirus on strawberries, green onions, and lettuce. J Food Prot 2008; 71(5): 908-913.
  10. Prikhodko VG, Sandoval-Jaime C, Abente EJ, et al. Genetic characterization of feline calicivirus strains associated with varying disease manifestations during an outbreak season in Missouri (1995-1996). Virus Genes 2014; 48(1): 96-110.
  11. Domingo E, Escarmís C, Sevilla N, et al. Basic concepts in RNA virus evolution. FASEB J 1996; 10(8): 859-864.
  12. Chen S, Liu D, Tian J, et al. Molecular characterization of HLJ-073, a recombinant canine coronavirus strain from China with an ORF3abc deletion. Arch Virol 2019; 164(8): 2159-2164.
  13. Decaro N, Mari V, Campolo M, et al. Recombinant canine coronaviruses related to transmissible gastroenteritis virus of Swine are circulating in dogs. J Virol 2009; 83(3): 1532-1537.
  14. Wang HM, Liu YG, Tang YD, et al. A natural recombinant PRRSV between HP-PRRSV JXA1-like and NADC30-like strains. Transbound Emerg Dis 2018; 65(4): 1078-1086.
  15. Liu D, Zhou R, Zhang J, et al. Recombination analyses between two strains of porcine reproductive and respiratory syndrome virus in vivo. Virus Res 2011; 155(2): 473-486.
  16. He CQ, He M, He HB, et al. The matrix segment of the "Spanish flu" virus originated from intragenic recombination between avian and human influenza A viruses. Transbound Emerg Dis 2019; 66(5): 2188-2195.
  17. Hu CJ, Chang WS, Fang ZS, et al. Nanoparticulate vacuolar ATPase blocker exhibits potent host-targeted antiviral activity against feline coronavirus. Sci Rep 2017; 7(1): 13043. doi: 10.1038/s41598-017-13316-0.
  18. Guo H, Miao Q, Zhu J, et al. Isolation and molecular characterization of a virulent systemic feline calicivirus isolated in China. Infect Genet Evol 2018; 65: 425-429.
  19. Meli ML, Berger A, Willi B, et al. Molecular detection of feline calicivirus in clinical samples: A study comparing its detection by RT-qPCR directly from swabs and after virus isolation. J Virol Methods 2018; 251: 54-60.
  20. Wu H, Zhang X, Liu C, et al. Antiviral effect of lithium chloride on feline calicivirus in vitro. Arch Virol 2015; 160(12): 2935-2943.
  21. Felsenstein J. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 1985; 39(4): 783-791.
  22. Zhou L, Fu N, Ding L, et al., Molecular characterization and cross-reactivity of Feline Calicivirus circulating in Southwestern China. Viruses 2021; 13(9): 1812. doi: 10.3390/v13091812.
  23. Lukashev AN. Recombination among picornaviruses. Rev Med Virol 2010; 20(5): 327-337.
  24. Zhao Y, Chen X, Ying Y, et al. Isolation and phylogenetic analysis of three feline calicivirus strains from domestic cats in Jilin Province, China. Arch Virol 2017; 162(9): 2579-2589.
  25. Liu C, Liu Y, Liu D, et al. Complete genome sequence of feline calicivirus strain HRB-SS from a cat in Heilongjiang Province, Northeastern China. Genome Announc 2014; 2(5): e00698-14. doi: 10.1128/ genomeA.00698-14
  26. Coyne KP, Reed FC, Porter CJ, et al. Recombination of feline calicivirus within an endemically infected cat colony. J Gen Virol 2006; 87(Pt 4): 921-926.
  27. Coyne KP, Gaskell RM, Dawson S, et al. Evolutionary mechanisms of persistence and diversification of a calicivirus within endemically infected natural host populations. J Virol 2007; 81(4): 1961-1971.
  28. Coyne KP, Christley RM, Pybus OG, et al. Large-scale spatial and temporal genetic diversity of feline calicivirus. J Virol 2012; 86(20): 11356-11367.
  29. Wei L, Huhn JS, Mory A, et al. Proteinase-polymerase precursor as the active form of feline calicivirus RNA-dependent RNA polymerase. J Virol 2001; 75(3): 1211-1219.
  30. Wu H, Zu S, Sun X, et al. N-terminal domain of feline calicivirus (FCV) proteinase-polymerase contributes to the inhibition of host cell transcription. Viruses 2016; 8(7): 199. doi: 10.3390/v8070199
  31. Wu H, Huang J, Liu Y, et al. Feline calicivirus proteinase-polymerase protein degrades mRNAs to inhibit host gene expression. J Virol 2021; 95(13): e00336-21. doi: 10.1128/JVI.00336-21.
  32. Simon-Loriere E, Holmes EC. Why do RNA viruses recombine? Nat Rev Microbiol 2011; 9(8): 617-626.
Veterinary Research Forum
Volume 14, Issue 11
November 2023
Pages 583-588

  • Receive Date 06 December 2022
  • Revise Date 19 March 2023
  • Accept Date 20 May 2023

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