Veterinary Research Forum
Vet Res Forum

Co-infection of bluetongue virus serotypes 12 and 16 in sheep from Haryana, India

Document Type : Original Article

Authors

Anita Dalal 1
Naresh Kumar Kakker 1
Deepika Chaudhary 2
Aman Kumar 2
Narender Singh Maan 3
Sushila Maan 2

1 Department of Veterinary Microbiology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

2 Department of Animal Biotechnology, College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

3 Department of Animal Nutrition College of Veterinary Sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India

https://doi.org/10.30466/vrf.2023.1990376.3796
Abstract
World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. The BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reported the dual infection of BTV serotypes, BTV-12 and -16 in sheep population from Sirsa district of Haryana in the year 2016. After detection and serotyping with Seg-2 specific real time polymerase chain reaction (PCR), the Seg-2 and Seg-6 of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in nucleotype G and nucleotype B specific for BTV-12 and BTV-16, respectively. This was the first report of BTV-16 from Haryana. The results signified the co-infection of two different serotypes in an animal from a single outbreak.

Keywords

Bluetongue virus
Dual infection
Goat
Molecular diagnosis
Real time RT-PCR

Subjects

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Veterinary Research Forum
Volume 14, Issue 12
December 2023
Pages 635-641

  • Receive Date 25 February 2023
  • Revise Date 17 May 2023
  • Accept Date 17 June 2023

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