Document Type : Original Article
Authors
- Sushila Maan 1
- Anita Dalal 2
- Naresh Kumar Kakker 3
- Deepika Chaudhary 4
- Aman Kumar 5
- Narender Singh Maan 6
1 Department of Animal Biotechnology, College of Veterinary sciences, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
2 Lala Lajpat Rai University of Veterinary and animal Sciences
3 Department of Veterinary Microbiology, College of Veterinary sciences, Lala Lajpat Rai University of Veterinary and Animal sciences, Hisar, Haryana
4 Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar,Haryana
5 Department of Animal Biotechnology, Lala Lajpat rai University of Veterinary and animal sciences, Hisarv, Haryana
6 Department of Animal Nutrition, Lala Lajpat Rai University of Veterinary and Animal sciences Hisar, Haryana.
Abstract
Abstract:
World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reports the dual infection of BTV serotypes, BTV-12 and -16 in sheep population from Sirsa district of Haryana in the year 2016. After detection and serotyping with Seg-2 specific real time PCR, the Seg-2 and Seg-6 of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in Nucleotype G and Nucleotype B specific for BTV-12 and BTV-16, respectively. This is the first report of BTV-16 from Haryana. The results signify the co-infection of two different serotypes in an animal from a single outbreak.
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