World Organization for Animal Health has listed bluetongue (BT) under notifiable diseases. BT is an arboviral infectious disease of domestic and wild ruminants caused by the bluetongue virus (BTV). Southern states of India had remained the point of attention for BT since first presence in 1964 in Maharashtra. Recently, northern states of India have also been reported positive for BTV in small ruminants. The present study reports the dual infection of BTV serotypes, BTV-12 and -16 in sheep population from Sirsa district of Haryana in the year 2016. After detection and serotyping with Seg-2 specific real time PCR, the Seg-2 and Seg-6 of BTV were PCR amplified and sequenced. On phylogenetic analysis it was detected to be clustered in Nucleotype G and Nucleotype B specific for BTV-12 and BTV-16, respectively. This is the first report of BTV-16 from Haryana. The results signify the co-infection of two different serotypes in an animal from a single outbreak.