Staphylococcus aureus is an important pathogen causing a wide range of diseases in both humans and animal. The aim of this research was to screen the vancomycin-resistant associated genes in methicillin-resistant Staphylococcus aureus (MRSA) isolates from animals. A total of 400 nasal swab samples were collected from cattle, goat and sheep between February and August 2022 from both industrial and traditional livestock farms in West Azerbaijan province, Iran. Then nasal swabs were cultured on mannitol salt agar and after bacteriological examination, molecular analysis was performed to confirm the presence of Staphylococcus aureus. The MecA gene was used to detect MRSA isolates, and two important vancomycin-resistance-associated genes, namely vanA and vanB were searched in the isolates. Out of 400 nasal swabs, 69 samples had S. aureus of which 7 isolates were resistant against methicillin. No vancomycin-resistance-associated genes were detected in the MRSA isolates. Based on these findings, vancomycin could be used to treat infections caused by this bacterium.