Document Type : Original Article


Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar, India


The present study investigated the prospect of improvement in pharmacokinetic (PK) parameters of marbofloxacin due to alpha-1-monolaurin pre-treatment in broiler chickens. Two groups of broilers were administered a single oral dose of marbofloxacin (5.00 mg kg-1 body weight): Group-I without pre-treatment and Group-II with alpha-1-monolaurin pre-treatment (4.00 g kg-1 feed for 10 days). Blood sampling was done periodically for both groups and plasma marbofloxacin concentrations were determined using ultra-high performance liquid chromatography. Pharmacokinetic parameters using non-compartmental modelling approach were calculated with the PKSolver software. Statistical analysis revealed significant differences in plasma marbofloxacin concentrations between the two groups at 1, 2, and 24 hr. Group-II birds exhibited a higher mean maximum plasma concentration (2.43 µg mL-1) at an earlier time (Tmax: 1.38 hr) compared to Group-I. The plasma concentrations of marbofloxacin were maintained above 0.10 and 0.18 µg mL-1 up to 24 hr in Group-I and Group-II broilers, respectively. Significant differences were observed in PK parameters such as the area under the curve and total body clearance. The mean relative oral bioavailability of Group-II birds compared to Group-I was 119.61%. The findings of the study provided evidence of PK parameters enhancement of marbofloxacin in the alpha-1-monolaurin pre-treated group. The calculated PK-pharmacodynamic indices for marbofloxacin predicted clinical efficaciousness in the broiler chickens.


Main Subjects

  1. OECD/Food and Agriculture Organization of the United Nations. In: OECD-FAO Agricultural Outlook 2022-2031. Paris, France: OECD Publishing 2022; 189-206.
  2. Gomez-Osorio LM, Yepes-Medina V, Ballou A, et al. Short and medium chain fatty acids and their derivatives as a natural strategy in the control of necrotic enteritis and microbial homeostasis in broiler chickens. Front Vet Sci 2021; 8: 773372. doi: 10.3389/ fvets.2021.773372.
  3. Dayrit FM, Newport MT. The potential of coconut oil and its derivatives as effective and safe antiviral agents against the novel coronavirus (nCOV-2019). Indian Coconut J 2022; 64(7): 23-26.
  4. Lieberman S, Enig MG, Preuss HG. A review of mono-laurin and lauric acid: natural virucidal and bactericidal agents. Altern Complement Ther 2006; 12(6): 310-314.
  5. Saleh AA, El-Gharabawy B, Hassan A, et al. Effect of dietary inclusion of alpha-monolaurin on the growth performance, lipid peroxidation, and immunity response in broilers. Sustainability 2021; 13(9): 5231. doi: 10.3390/su13095231.
  6. Mustafa NG. Biochemical trails associated with different doses of alpha-monolaurin in chicks. Adv Anim Vet Sci 2019; 7(3): 187-192.
  7. Letlole BR, Damen EPCW, van Rensburg CJ. The effect of α-monolaurin and butyrate supplementation on broiler performance and gut health in the absence and presence of the antibiotic growth promoter zinc bacitracin. Antibiotics (Basel) 2021; 10(6): 651. doi: 10.3390/antibiotics10060651.
  8. Singh RD, Vaghela SH, Tukra S, et al. Dosage derivation of marbofloxacin in broiler chickens based on pharma-cokinetic-pharmacodynamic integration. Indian J Vet Sci Biotechnol 2023; 19(2): 7-11.
  9. Haritova AM, Rusenova NV, Parvanov PR, et al. Integration of pharmacokinetic and pharmacodynamic indices of marbofloxacin in turkeys. Antimicrob Agents Chemother 2006; 50(11): 3779-3785.
  10. Roth N, Käsbohrer A, Mayrhofer S, et al. The application of antibiotics in broiler production and the resulting antibiotic resistance in Escherichia coli: a global overview. Poultry Sci 2019; 98(4): 1791-1804.
  11. Nhung NT, Chansiripornchai N, Carrique-Mas JJ. Anti-microbial resistance in bacterial poultry pathogens. Front Vet Sci 2017; 4: doi: 10.3389/fvets.2017.00126.
  12. Patel HB, Patel UD, Modi CM, et al. Pharmacokinetic profiles of marbofloxacin following single and repeated oral administration in broiler chickens. Ann Phytomed 2018; 7(2): 174-179.
  13. Carpenter JW, Hunter RP, Olsen JH, et al. Pharmaco-kinetics of marbofloxacin in blue and gold macaws (Ara ararauna). Am J Vet Res 2006; 67(6): 947-950.
  14. Zhang Y, Huo M, Zhou J, et al. PKSolver: an add-in program for pharmacokinetic and pharmacodynamic data analysis in Microsoft Excel. Comput Methods Programs Biomed 2010; 99(3): 306-314.
  15. Wesch R. Absolute and relative bioavailability. In: Vogel HG, Maas J, Gebauer A (Eds). Drug discovery and evaluation: methods in clinical pharmacology. Berlin, Germany: Springer 2011; 173-180.
  16. Anadón A, Martínez MR, Díaz MJ, et al. Pharmacokinetic characteristics and tissue residues for marbofloxacin and its metabolite N-desmethyl-marbofloxacin in broiler chickens. Am J Vet Res 2002; 63(7): 927-933.
  17. El-Komy A, Attia T, El Latif AA, et al. Bioavailability pharmacokinetics and residues of marbofloxacin in normal and coli infected broiler chicken. Int J Pharmacol Toxicol 2016; 4(2): 144-149.
  18. Atef M, Atta A, Darwish AS, et al. Pharmacokinetics aspects and tissue residues of marbofloxacin in healthy and Mycoplasma gallisepticum-infected chickens. Wulfenia 2017; 24(10): 80-107.
  19. Martinez MN, Amidon GL. A mechanistic approach to understanding the factors affecting drug absorption: a review of fundamentals. J Clin Pharmacol 2002; 42(6): 620-643.
  20. DeHaven WI, Conner DP. The effects of food on drug bioavailability and bioequivalence. In: Lawrence XY, Bing VL (Eds). FDA bioequivalence standards. New York, USA: Springer 2014; 95-118.
  21. Singh RD, Tukra S, Patel HB, et al. Pharmacology and prospects of alpha-1-monolaurin as an alternative growth promoter in poultry industry. J Vet Pharmacol Toxicol 2022; 21(2): 1-6.
  22. Patel A, Patel HB, Sarvaiya VN, et al. Pharmacokinetics of marbofloxacin following oral administration in lactic acid pretreated broiler chickens. Asian J Dairy Food Res 2023; doi:10.18805/ajdfr.DR-2046.
  23. Aboubakr M, Abdelazem AM. Pharmacokinetics of marbofloxacin in Japanese quails (Coturnix japonica) after different routes of administration. J Am Sci 2015; 11(4): 136-142.
  24. Drlica K, Zhao X. Mutant selection window hypothesis updated. Clin Infect Dis 2007; 44(5): 681-688.
  25. Rajgor NS, Mody SK, Patel HB, et al. Intravenous and oral pharmacokinetics of marbofloxacin in layer birds. J Vet Pharmacol Toxicol 2019; 18(2): 10-14.
  26. Nielsen EI, Friberg LE. Pharmacokinetic-pharmaco-dynamic modeling of antibacterial drugs. Pharmacol Rev 2013; 65(3): 1053-1090.
  27. Spreng M, Deleforge J, Thomas V, et al. Antibacterial activity of marbofloxacin. A new fluoroquinolone for veterinary use against canine and feline isolates. J Vet Pharmacol Ther 1995; 18(4): 284-289.
  28. Kroemer S, Galland D, Guérin-Faublée V, et al. Survey of marbofloxacin susceptibility of bacteria isolated from cattle with respiratory disease and mastitis in Europe. Vet Rec 2012; 170(2): 53. doi: 10.1136/vr.100246.