Document Type : Original Article

Authors

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

2 Associate Professor, Department of Clinical Sciences, Faculty of Veterinary Medicine,Shahid Chamran University of Ahvaz- Iran ,

3 Assistant Professor of Medical Virology, Department of Laboratory Sciences, School of Allied Medical Sciences Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

4 Professor, Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz

Abstract

An internationally identified syndrome that leads to deaths between domestic and ornamental pigeons, particularly after racing is Young Pigeon Disease Syndrome (YPDS). Pigeon circovirus (PiCV) is regarded as one of the potential factors in the occurrence of YPDS. This survey was conducted to determine the status of PiCV infection and molecularly characterize the PiCV in pigeons suspected of YPDS. In this study, from 20 lofts in different areas of Ahvaz, 80 fecal samples from 80 diseased pigeons (with symptoms of lethargy, weight loss, crop stasis, vomiting, and diarrhea), as well as 20 fecal samples from 20 clinically healthy pigeons were obtained. To identify the circovirus, the Nested Broad Spectrum PCR (Nested-PCR) test was done using primers that target part of the rep gene with 350 bp, and several positive samples were sequenced. This study showed that PiCV was detected in 86 samples (86%). Two types of circoviruses were determined in samples. One type of the detected circoviruses was PiCVs which based on phylogenetic analysis had high genetic similarity with PiCV genotypes A, B, G, and H. Interestingly, the other type of detected circoviruses was closely related to BFDV which causes one of the most important viral diseases in psittacine birds. This is the first report on the identification of BFDV in pigeons.

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