Veterinary Research Forum
Vet Res Forum

Genetic characterization of virulence and extended spectrum β-lactamase producing genes of Klebsiella pneumoniae isolated from bovine milk

Document Type : Original Article

Authors

Bhavinkumar Pankajbhai Katira 1
Bhaveshkumar Ishwarbhai Prajapati 1
Ratn Deep Singh 2
Sandipkumar Sureshbhai Patel 3
Kirankumar Motiji Solanki 1

1 Department of Veterinary Public Health and Epidemiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar, India

2 Department of Veterinary Pharmacology and Toxicology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar, India

3 Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Kamdhenu University, Sardarkrushinagar, India

https://doi.org/10.30466/vrf.2023.2006974.3947
Abstract
Mastitis associated Klebsiella pneumoniae species were isolated from bovine milk to characterize virulence genes (wabG and kfuBC) and extended spectrum β-lactamase (ESBL) genes (blaCTX-M-1, blaCTX-M-2, blaCTX-M-9, blaTEM, blaSHV and blaOXA). A total number of 325 bovine milk samples (195 raw and 130 mastitic milk specimens) collected from Banaskantha, a milk-shed district of Gujarat, India, were included in the study. A total number of 27 K. pneumoniae isolates were recovered, consisting of 17 (62.96%) isolates from raw milk and 10 (37.03%) isolates from mastitic milk samples, giving an overall prevalence of 8.31%. Antibiotic sensitivity patterns revealed that 20 out of 27 isolates were found to be multi-drug resistant. Based on combination disc diffusion test and HiCrome ESBL agar method, 20 (74.07%) and 25 (92.59%) isolates were detected as ESBL producers, respectively. Among virulence genes studied, presence of wabG (25/27; 92.59%) was higher than kfuBC (5/27; 18.51%). Beta-lactamase genes viz., blaSHV, blaTEM and blaCTX-M-1 were detected in 23/27 (85.18%), 3/27 (11.11%) and 2/27 (7.40%) of isolates, respectively; while, none of the isolates was found to be positive for blaCTX-M-9 and blaOXA-1 genes. Outcome of the study provided an insight into virulence genes and ESBL producing K. pneumoniae isolated from bovine milk samples in India.

Keywords

Bovine mastitis
Drug resistance
India
Klebsiella pneumoniae
Virulence factors
Subjects
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Veterinary Research Forum
Volume 15, Issue 2
February 2024
Pages 57-64

  • Receive Date 20 July 2023
  • Revise Date 29 August 2023
  • Accept Date 23 September 2023

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