Veterinary Research Forum
Vet Res Forum

Anti-Listeria activity of postbiotics of Lactiplantibacillus sakei in beef fillet using aerosolization technique

Document Type : Original Article

Authors

Sima Valipour 1
Hossein Tajik 2
Mehran Moradi 2
Rahim Molaei 3
Mahdi Ghorbani 4

1 PhD Candidate of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

3 Urmia University

4 Graduate of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

https://doi.org/10.30466/vrf.2023.2014356.4044
Abstract
In recent years, the use of probiotics and their metabolites, known as postbiotics as natural preservatives has received increasing attention in the food industry. This study aimed to prepare and characterize postbiotics of Lactiplantibacillus sakei and to investigate its application as an anti-Listeria solution on beef fillets using an aerosolization technique. The functional groups, including organic acids, polysaccharides and other minor metabolites, were identified by Fourier transform infrared (FTIR) in the postbiotics. The 2, 2′-diphenyl-1-picrylhydrazyl radical scavenging activity of the postbiotics was reported as 0.82 mg mL-1. The antimicrobial test using the agar well diffusion method revealed a zone of inhibition of 27.00 ± 1.20 mm. Application of an aerosolized postbiotics solution resulted in a significant reduction in Listeria monocytogenes counts on beef fillets, reaching 3.30 log10 CFU g-1 over a 15-day storage period at 4.00 ± 1.00 ˚C. The results of this study revealed that the postbiotics of L. sakei was an effective antimicrobial additive for controlling foodborne pathogens in beef fillets and aerosolization is a promising method for developing an antimicrobial coating on meat to enhance meat safety.

Keywords

Aerosolization
Active packaging
Food shelf life
Lactiplantibacillus sakei
Postbiotics

Subjects

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Veterinary Research Forum
Volume 15, Issue 5
May 2024
Pages 223-229

  • Receive Date 25 October 2023
  • Revise Date 25 November 2023
  • Accept Date 02 December 2023

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