Veterinary Research Forum
Vet Res Forum

Detection of antibodies against structural proteins of foot-and-mouth disease virus in bovines of western Uttar Pradesh, India

Document Type : Original Article

Authors

Afroz Afroz 1
Amit Kumar Verma 2
Amit Kumar 3
Surendra Upadhyay 4
Arbind Singh 5

1 Veterinary Officer, Animal Husbandry Department, District Mainpuri, India

2 Department of Veterinary Medicine, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut , India

3 Animal Biotechnology, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India

4 PhD Scholar, College of Biotechnology, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India

5 Livestock Farm Complex, College of Veterinary and Animal Sciences, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut, India

https://doi.org/10.30466/vrf.2024.2014839.4057
Abstract
Foot-and-mouth disease (FMD) is considered as one of the most important contagious viral diseases affecting cloven-footed animals. For effective control of FMD, immunization along with herd immunity is essential in the field conditions. To assure and track the coverage and effectiveness of the vaccination program, the serological studies are very much required after the vaccination program. The present study was aimed to investigate the prevalence of antibodies against structural proteins of FMD virus (FMDV) serotypes of O, A and Asia-1 in seven districts of western Uttar Pradesh, India, and assure the efficacy of vaccination under National Animal Disease Control Program. A total of 308 sera samples were collected from apparent healthy vaccinated cattle and buffaloes from seven districts including Amroha, Baghpat, Bareilly, Bulandsahar, Gautam Budh Nagar, Meerut and Muzaffarnagar of western Uttar Pradesh, India. Determination of antibodies against structural proteins of FMDV was carried out using solid-phase blocking enzyme-linked immunosorbent assay. The protective level of the FMDV serotypes O, A and Asia-1 included in the inactivated trivalent vaccine was 66.55, 48.05 and 47.08% in bovines, respectively. To provide the higher level of protection against the circulating FMDV, the present study recommended the thorough investigation of the immunogenic interaction between the vaccine strains and the field strains. Further investigations should also be conducted with larger sample size and across diverse geographical regions to gain a more comprehensive understanding of herd immunity.

Keywords

Foot-and-mouth disease virus
India
Solid-phase blocking ELISA
Structural protein

Subjects

  1. Abd El-Rhman MM, Abo El-Hassan DG, Awad WS, et al. Serological evaluation for the current epidemic situation of foot and mouth disease among cattle and buffaloes in Egypt. Vet World 2020; 13(1): 1-9.
  2. Verma AK, Kumar A, Mahima M, et al. Epidemiology and diagnosis of foot-and-mouth disease: a review. Indian J Anim Sci 2012; 82(6): 543-551.
  3. Chakraborty S, Kumar N, Dhama K, et al. Foot-and-mouth disease, an economically important disease of animals. Adv Anim Vet Sci 2014; 2(2S): 1-18.
  4. Afroz A, Verma AK, Kumar A, et al. Seroprevalence and associated risk factors of foot and mouth disease in western Uttar Pradesh. Biol Forum Int J 2023; 15(1): 448-454.
  5. Singh A, Yadav SK, Verma AK, et al. Prevalence and distribution of foot and mouth disease virus serotypes in bovines of Uttar Pradesh. Multilogic Sci 2019; 8: 38-40.
  6. Hamblin C, Barnett IT, Hedger RS. A new enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. I. Development and method of ELISA. J Immunol Methods1986; 93(1): 115-121.
  7. Hamblin C, Kitching RP, Donaldson AI, et al. Enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and-mouth disease virus. III. Evaluation of antibodies after infection and vaccination. Epidemiol Infect 1987; 99(3): 733-744.
  8. Pattnaik B, Venkataramanan R. Comparison of liquid-phase and Mab-blocking ELISA for assessment of the reactivity of monoclonal antibodies to foot-and-mouth disease virus. J Immunol Methods 1994; 172(2): 265-267.
  9. Singh CP, Verma AK, Pal BC. Prevalence of protected animals against FMD in Uttar Pradesh. Haryana Vet 2008; 47: 107-109.
  10. Mackay DK, Bulut AN, Rendle T, et al. A solid-phase competition ELISA for measuring antibody to foot and mouth disease virus. J Virol Methods 2001; 97(1-2): 33-48.
  11. Paiba GA, Anderson J, Paton DJ, et al. Validation of a foot-and-mouth disease antibody screening solid-phase competition ELISA (SPCE). J Virol Methods 2004; 115(2): 145-158.
  12. Ma LN, Zhang J, Chen HT, et al. An overview on ELISA techniques for FMD. Virol J 2011; 8: 419.doi: 10.1186/1743-422X-8-419.
  13. Verma AK, Pal BC, Singh CP, et al. Studies of the outbreaks of foot and mouth disease in Uttar Pradesh, India, between 2000 and 2006. Asian J Epidemiol 2008; 1(2): 40-46.
  14. Verma AK, Sehzad S, Mehra S, et al. LPB ELISA based pre and post-vaccination seroprevalence of foot and mouth disease virus. Indian J Comp Microbiol Immunol Infect Dis 2009; 30(2): 130-132.
  15. Subramaniam S, Pattnaik B, Sanyal A, et al. Status of foot-and-mouth disease in India. Transbound Emerg Dis 2013; 60(3): 197-203.
  16. Hegde R, Kowalli S, Nagaraja K, et al. Serosurveillance of foot and mouth disease in Karnataka state, India: a 3 years study. Virus Dis 2016; 27(3): 294-302.
  17. Doel TR. Optimization of the immune response to foot-and-mouth disease vaccines. Vaccine 1999; 17(13-14): 1767-1771.
  18. Garland AJ. Vital elements for the successful control of foot-and-mouth disease by vaccination. Vaccine 1999; 17(13-14): 1760-1766.
  19. Verma AK, Tripathi AK, Neha N. Investigation of FMD outbreak in district Bulandshahr, Uttar Pradesh, India. Int J Livest Res 2017; 7(4): 113-118.
  20. Bora M, Sharma R, Kakker NK. Detection of anti-non-structural protein antibodies against foot and mouth disease virus in the bovine population of Haryana during FMD control programme in the year 2012. Haryana Vet 2014; 53(1): 8-12.
Veterinary Research Forum
Volume 15, Issue 5
May 2024
Pages 219-222

  • Receive Date 04 November 2023
  • Revise Date 21 December 2023
  • Accept Date 05 February 2024

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