Veterinary Research Forum
Vet Res Forum

Molecular characterization of antimicrobial resistance genes of Staphylococcus aureus isolated from mastitic camel milk in Egypt

Document Type : Original Article

Authors

Mohamed Mohamed Ali 1
Salwa Mahmoud Helmy 1
Hanan Ali Fahmy 2
Haitham Elaadli 3
Ibrahim Elsayed Eldesoukey 1

1 Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt

2 Department of Biotechnology, Animal Health Research Institute, Agricultural Research Center, Giza, Egypt

3 Department of Animal Hygiene and Zoonoses, Faculty of Veterinary Medicine, Alexandria University, Alexandria, Egypt

https://doi.org/10.30466/vrf.2023.2007392.3946
Abstract
Staphylococcus aureus is one of the most common causes of mastitis worldwide. This study aimed to determine the prevalence and antimicrobial resistance (AMR) patterns of S. aureus in mastitic milk samples collected from camel farms in Matrouh Governorate, Egypt. A total of 200 mastitic camel milk samples were evaluated for S. aureus using both conventional culture-based and molecular-based methods. Antibiotic susceptibility testing of S. aureus isolates was conducted using disc diffusion and agar dilution methods, with antibiotic resistance genes identified through polymerase chain reaction with specific primers. Out of samples tested, 60 (30.00%) were positive for S. aureus. The isolates displayed the highest of resistance against piperacillin-tazobactam (55.00%) followed by trimethoprim- sulfamethoxazole (45.00%) and amoxicillin (40.00%). Half of the isolates were multidrug-resistant (MDR). The AMR genes included methicillin-resistant gene (mecA), β-lactamase gene (blaZ), tetracycline resistance gene (tetK), erythromycin resistance gene (ermB) and vancomycin resistant gene (vanA) were detected in 100%, 100%, 95.00%, 90.00% and 20.00% of the isolates, respectively. In conclusion, the presence of MDRS aureus as a cause of clinical camel mastitis is a significant veterinary and public health concern. These findings highlight the importance of proper antibiotic use in Egyptian camel farms and the need for molecular techniques to fully understand the genetic profile of antimicrobial-resistant S. aureus isolates.

Keywords

Antimicrobial resistance genes
Camel
Egypt
Mastitis
Staphylococcus aureus

Subjects

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Veterinary Research Forum
Volume 15, Issue 6
June 2024
Pages 267-274

  • Receive Date 19 July 2023
  • Revise Date 07 November 2023
  • Accept Date 02 December 2023

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