Document Type : Original Article
Authors
- Ahmad reza raji 1
- shadi Hashemnia 2
- Ahmad ail Mohammadpour 3
- Abolghasem Nabipour 4
- zohre saddatfar 5
1 Department of basic sciences, Faculty of veterinary medicine, ferdowsi university of Mashhad, Mashhad, Iran
2 Department of basic sciences, veterinary faculty of shahid bahonat university of kerman. Iran
3 Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
4 Department of basic sciences, Faculty of veterinary medicine, Ferdowsi University of Mashhad, Mashhad, Iran
5 Department of Basic sciences, Faculty of Veterinary medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Ostrich (Struthio camelus) is an important wild species highlighted in national and international livestock. This research was conducted to analyze the development of the ostrich respiratory system in fetal and embryonic stages.
A total of 50 fertile ostrich eggs were collected from commercial farms. They were incubated at 36–37°C and 25 ± 2% humidity for 40 days. Sections were taken in 13, 22, 26, 30, 36, and 42 days of incubation from the lung and the cranial, middle, and caudal parts of the neck after decapitation of ostrich embryos and blood drainage. After fixation, processing, blocking, and sectioning, all samples were stained by hematoxylin and eosin, Alcian blue (AB), Van Gieson, and Periodic acid-Schiff (PAS) techniques.
It was concluded that the trachea in the 13-day-old embryo (E13) and goblet cells (PAS-positive and AB-positive) had incomplete rings of hyaline cartilages and differentiation of mesenchymal to the loose connective tissue.
Bronchial stage of the lung in the E22, pseudo-glandular stage in the E26, and parabrachial and air capillary stage in the E30 were also observed. The information obtained here will be useful for diagnosing the pathologies affecting this vital system and results in improving industrial breeding management.
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