Document Type : Original Article


1 Associate Professor, Department of veterinary microbiology, NTR College of Veterinary Science,Gannavaram

2 Department of Veterinary Microbiology, College of Veterinary Science, Tirupati

3 Department of Animal Genetics and Breeding, Sri Venkateswara Veterinary University, College of Veterinary Science, Garividi, Andhra Pradesh, India

4 Livestock Farm Complex, Sri Venkateswara Veterinary University N.T.R College of Veterinary Science, Gannavaram

5 Department of Veterinary Microbiology, Sri Venkateswara Veterinary University N.T.R College of Veterinary Science, Gannavaram



The aim of this study was to investigate the probiotic potential of autochthonous Lactobacillus species isolated from buffalo calves against multidrug-resistant Escherichia. coli (E. coli). A total of 252 rectal swabs were collected from healthy neonatal buffalo calves under 30 days old from six districts of Andhra Pradesh, India in a completely randomized design for a period from August 2019 to August 2021, of which 190 Lactobacillus were isolated based on cultural, morphological, biochemical and molecular testes. Among these isolates, 57 showed a high degree of auto-aggregation (>80%) and hydrophobicity (>60%) and 51 of the 57 isolates had a zone diameter of inhibition more than 15 mm against multidrug-resistant E. coli in Agar well diffusion assay. Out of 51 isolates, 36 were found to be acid and bile tolerant and showed varying levels of sensitivity to the antibiotics such as erythromycin, clindamycin, tetracycline, chloramphenicol, and ampicillin. Among the 36 isolates, L. reuteri 178, L. reuteri 209, L. fermentum 182, L. fermentum 211, and L. plantarum 34 were non-haemolytic, and none of the isolates were able to hydrolyse gelatine. Therefore, these five autochthonous Lactobacillus species may be used in probiotic or synbiotic formulations against multidrug resistant E. coli in buffalo calves.


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