Veterinary Research Forum
Vet Res Forum

Preparation and evaluation of controlled released implant containing mesoporous selenium nanoparticles loaded with curcumin in rats with spinal cord injury

Document Type : Original Article

Authors

Ehsan Lajmiri 1
Moosa Javdani 2
Pegah Khosravian 3
Mohammad Hashemnia 4
Hossein Kazemi Mehrjerdi 1

1 Department of Clinical Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahrekord University, Shahrekord, Iran

3 Medical Plants Research Center, Basic Health Science Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

4 Department of Pathobiology, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran

https://doi.org/10.30466/vrf.2024.2014162.4040
Abstract
In this study, a controlled released delivery drug system designed and synthesized by loading curcumin and selenium nanoparticles (SeNaPs) on chitosan hydrogel, and while evaluating the physicochemical properties of the prepared drug delivery system, the tissue changes caused by the local implant of that system in rats with experimental spinal cord injury (SCI) were investigated. For this purpose, 100 adult female rats were randomly divided into five equal groups which are: Control group without any treatment for SCI, chitosan group that received chitosan hydrogel, curcumin group that received curcumin-loaded hydrogel, SeNaP group that received chitosan loaded with SeNaPs and SeNPCur group that received chitosan loaded with SeNaPs and curcumin. On the 3rd and 7th days of the study, severe infiltration of leukocytes, especially lymphocytes, as well as axon swelling and hemorrhagic necrosis at the lesion sites were observed in all groups, especially the control group. On the 7th day, the severity of these injuries decreased in the SeNPCur group and the highest number of astrocytes was observed in this group. In addition, on the 14th and 21st days of the study, the lowest severity of nerve tissue damage and the lowest presence of inflammatory cells along with the highest number of astrocytes were seen in the SeNPCur group. The glial fibrillary acidic protein study also confirmed the presence of more and significant astrocytes in the SeNPCur, curcumin and SeNP groups at different times of the study, respectively. The histopathological results showed the neuroprotective effects of chitosan hydrogel loaded with selenium and curcumin.

Keywords

Chitosan Hydrogel
Curcumin
Spinal cord injury
Selenium nanoparticles
Subjects
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Veterinary Research Forum
Volume 15, Issue 7
July 2024
Pages 357-367

  • Receive Date 31 October 2023
  • Revise Date 05 January 2024
  • Accept Date 04 March 2024

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