Veterinary Research Forum
Vet Res Forum

Epigallocatechin-3-gallate affects the quality of fresh and frozen-thawed semen of Simmental bull by two different cryopreservation methods

Document Type : Original Article

Authors

Abolfazl Parvizi Alan 1
Esmail Ayen 1
Amir Khaki 2
Ali Soleimanzadeh 1

1 Department of Theriogenology and Poultry Disease, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

2 Department of Clinical Sciences, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran

https://doi.org/10.30466/vrf.2024.2000236.3852
Abstract
During the freezing process of semen, due to the generating of significant amounts of free radicals, the quality of sperm changes. Epigallocatechin-3-gallate (EGCG) is a green tea catechin, which in this study was applied to investigate its effect on the quality of bulls' sperm. We collected semen samples with an artificial vagina from 12 Simmental bulls to evaluate the effect of EGCG (10.00 and 20.00 µmol) in two cryopreserving methods on the quality parameters of semen. We designed six groups including two control groups (method one and two) and four treatments (EGCG 10.00 µmol + method one; EGCG 20.00 µmol + method one; EGCG 10.00 µmol + method two; EGCG 20.00 µmol + method two). The 20.00 µmol EGCG and a method two significantly affected the amending oxidative conditions as well as an increase in total antioxidant capacity and a decrease in malondialdehyde. The effect of EGCG in both concentrations was more on method two. The desired impact on sperm motility, viability, inhibition of lipid peroxidation and sperm DNA damage was observed in EGCG groups compared to control groups. Among the two methods, the method two had fewer adverse effects on the plasma membrane, motility parameters, viability and DNA of sperm. The EGCG in the semen extender yielded a favorable impact on thawed sperm. This effect was prompted in combination with the method two.

Keywords

Epigallocatechin-3-gallate
Semen preservation
Simmental
Sperm quality

Subjects

  1. Ugur MR, Saber Abdelrahman A, Evans HC, et al. Advances in cryopreservation of bull sperm. Front Vet Sci 2019; 6: 268. doi: 10.3389/fvets.2019.00268.
  2. Jang TH, Park SC, Yang JH, et al. Cryopreservation and its clinical applications. Integr Med Res 2017; 6(1): 12-18.
  3. Yánez-Ortiz I, Catalán J, Rodríguez-Gil JE, et al. Advances in sperm cryopreservation in farm animals: cattle, horse, pig and sheep. Anim Reprod Sci 2022; 246: 106904. doi: 10.1016/j.anireprosci.2021.106904.
  4. Galarza DA, López-Sebastián A, Woelders H, et al. Two-step accelerating freezing protocol yields a better motility, membranes and DNA integrities of thawed ram sperm than three-steps freezing protocols. Cryobiology 2019; 91: 84-89.
  5. Isachenko V, Rahimi G, Mallmann P, et al. Technologies of cryoprotectant-free vitrification of human spermatozoa: asepticity as criterion of effectiveness. Andrology 2017; 5(6): 1055-1063.
  6. Ros-Santaella JL, Pintus E. Plant extracts as alternative additives for sperm preservation. Antioxidants (Basel) 2021; 10(5): 772. doi: 10.3390/antiox10050772.
  7. Lourenço SC, Moldão-Martins M, Alves VD. Antioxidants of natural plant origins: from sources to food industry applications. Molecules 2019; 24(22): 4132. doi: 10.3390/molecules24224132.
  8. Prasanth MI, Sivamaruthi BS, Chaiyasut C, et al. A review of the role of green tea (Camellia sinensis) in antiphotoaging, stress resistance, neuroprotection, and autophagy. Nutrients 2019; 11(2): 474. doi: 10.3390/ nu11020474.
  9. Plaza Dávila M, Bucci D, Galeati G, et al. Epi-gallocatechin‐3‐gallate (EGCG) reduces rotenone effect on stallion sperm–zona pellucida heterologous binding. Reprod Domest Anim 2015; 50(6): 1011-1016.
  10. Bucci D, Spinaci M, Yeste M, et al. Combined effects of resveratrol and epigallocatechin-3-gallate on post thaw boar sperm and IVF parameters. Theriogenology 2018; 117: 16-25.
  11. Bucci D, Cunto M, Gadani B, et al. Epigallocatechin-3-gallate added after thawing to frozen dog semen: effect on sperm parameters and ability to bind to oocytes’ zona pellucida. Reprod Biol 2019; 19(1): 83-88.
  12. Chen M, Liu W, Li Z, et al. Effect of epigallocatechin-3-gallate (EGCG) on embryos inseminated with oxidative stress-induced DNA damage sperm. Syst Biol Reprod Med 2020; 66(4): 244-254.
  13. Kaedei Y, Naito M, Naoi H, et al. Effects of (-)‐epi-gallocatechin gallate on the motility and penetrability of frozen-thawed boar spermatozoa incubated in the fertilization medium. Reprod Domest Anim 2012; 47(6): 880-886.
  14. Jha PK, Shahi Alam MG, Mansur AA, et al. Cryo-preservation of Bangladeshi ram semen using different diluents and manual freezing techniques. Cryobiology 2019; 89: 35-41.
  15. Arif AA, Maulana T, Kaiin EM, et al. Comparative analysis of various step-dilution techniques on the quality of frozen Limousin bull semen. Vet World 2020; 13(11): 2422-2428.
  16. Schulze M, Kuster C, Schäfer J, et al. Effect of production management on semen quality during long-term storage in different European boar studs. Anim Reprod Sci 2018; 190: 94-101.
  17. Götz KU, Luntz B, Robeis, et al. Polled Fleckvieh (Simmental) cattle - current state of the breeding program. Livest Sci 2015; 179: 80-85.
  18. Björndahl L, Kirkman Brown J; et al. The sixth edition of the WHO Laboratory Manual for the Examination and Processing of Human Semen: ensuring quality and standardization in basic examination of human Fertil Steril 2022; 117(2): 246-251.
  19. Ramazani N, Mahd Gharebagh F, Soleimanzadeh A, et al. Reducing oxidative stress by κ-carrageenan and C60HyFn: The post-thaw quality and antioxidant status of Azari water buffalo bull semen. Cryobiology 2023; 111: 104-112.
  20. Ijaz A, Hussain A, Aleem M, et al. Butylated hydroxy-toluene inclusion in semen extender improves the post-thawed semen quality of Nili-Ravi buffalo (Bubalus bubalis). Theriogenology 2009; 71(8): 1326-1329.
  21. Kim GY. What should be done for men with sperm DNA fragmentation? Clin Exp Reprod Med 2018; 45(3): 101-109.
  22. Khaki A, Araghi A, Lotfi M, et al. Differences between first and second ejaculations regarding seminal plasma calcium, magnesium and total antioxidant capacity in dual-purpose Fleckvieh bulls and their relationships with semen quality. Vet Res Forum 2019; 10(4): 333-341.
  23. Dwinofanto H, Rimayanti R, Mustofa E, et al. The effect of duration of preservation on the quality, MDA level, and DNA damage of post-thawed Bali cattle bull sperm. Iraq J Vet Sci 2019; 32(2): 249-252.
  24. Uavechanichkul R, Pongpeng J, Wirojwutthikul S, et al. Quality of bull deep frozen semen extended with different glycerol concentrations: evaluated by computer assisted semen analysis. J Kasetsart Vet 2010: 20: 53-62.
  25. Khan H, Khan M, Qureshi MS, et al. Effect of green tea extract (Camellia sinensis) on fertility indicators of post-thawed bull spermatozoa. Pakistan J Zool 2017; 49(4): 1243-1249.
  26. Mizera A, Kuczaj M, Szul A. Impact of the Spirulina maxima extract addition to semen extender on bovine sperm quality. Ital J Anim Sci 2019; 18(1): 601-607.
  27. Nath RG, Wu MY, Emami A, et al. Effects of epigallocatechin gallate, L-ascorbic acid, alpha-tocopherol, and dihydrolipoic acid on the formation of deoxyguanosine adducts derived from lipid peroxidation. Nutr Cancer 2010; 62(5): 622-629.
  28. Zubair M, Ali M, Ahmad M, et al. Effect of selenium and vitamin E on cryopreservation of semen and reproductive performance of animals (a review). J Entomol Zool Stud 2015; 3(1): 82-86.
  29. Agarwal A, Varghese AC, Sharma RK. Markers of oxidative stress and sperm chromatin integrity. Methods Mol Biol 2009; 590: 377-402.
  30. Lewis SE, Aitken RJ. DNA damage to spermatozoa has impacts on fertilization and pregnancy. Cell Tissue Res 2005; 322(1): 33-41.
  31. Fraser L, Strzezek J. Is there a relationship between the chromatin status and DNA fragmentation of boar spermatozoa following freezing-thawing? Theriogenology 2007; 68(2): 248-257.
  32. Gliozzi TM, Zaniboni L, Cerolini S. DNA fragmentation in chicken spermatozoa during cryopreservation. Theriogenology 2011; 75(9): 1613-1622.
  33. Bogle OA, Kumar K, Attardo-Parrinello C, et al. Identification of protein changes in human spermatozoa throughout the cryopreservation process. Andrology 2017; 5(1): 10-22.
  34. ChaithraShree AR, Ingole SD, Dighe VD, et al. Effect of melatonin on bovine sperm characteristics and ultrastructure changes following cryopreservation. Vet Med Sci 2020; 6(2): 177-186.
  35. Ahmed H, Jahan S, Khan A, et al. Supplementation of green tea extract (GTE) in extender improves structural and functional characteristics, total antioxidant capacity and in vivo fertility of buffalo (Bubalus bubalis) bull spermatozoa. Theriogenology 2020; 145: 190-197.
  36. Ahmed H, Jahan S, Riaz M, et al. Epigallocatechin-3-gallate (EGCG) addition as an antioxidant in a cryo-diluent media improves microscopic parameters, and fertility potential, and alleviates oxidative stress parameters of buffalo spermatozoa. Cryobiology 2020; 97: 101-109.
Veterinary Research Forum
Volume 15, Issue 7
July 2024
Pages 369-377

  • Receive Date 16 April 2023
  • Revise Date 21 February 2024
  • Accept Date 07 April 2024

Home

Guide for Authors

Submit Manuscript

Reviewers

Contact Us