Veterinary Research Forum
Vet Res Forum

Evaluating potential of H’mong chicken bone marrow-derived mesenchymal stem cells at different ages for primordial germ cells’ feeder layer

Document Type : Original Article

Authors

Van Hanh Nguyen
Thi Huong-Giang Tran
Hong Thi Nguyen
Hiep Thi Nguyen
Nhung Thi Nguyen
Tien Phat Do

Department of Embryo Technology, Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, Vietnam

https://doi.org/10.30466/vrf.2024.2016008.4070
Abstract
Primordial germ cells (PGCs) have potential applications in genetic conservation, vaccination, tissue repair therapies, and genetic research. Chicken bone marrow-derived mesenchymal stem cells (cbMSCs) is a good candidate for co-culture with PGCs. However, there is no consensus on the optimal age of donors. In this study, we aimed to compare specific parameters of H’Mong cbMSCs obtained from day 14th and 19th embryos, and day 3rd newborns. Isolated cbMSCs showed characteristics of MSCs. Cells had fibroblast-like morphology, plastic-adherent, expressed specific markers of MSCs and multilineage differentiation potential. The growth rate of cells from day 19th embryos was higher than from other ages. Moreover, cells expressed markers of pluripotency such as Nanog, PouV, Sox2, CVH, DAZL, and KIT, known for their role in maintaining stem cell self-renewal and pluripotency. As feeder cells, cbMSCs from three different ages promoted proliferation of H’Mong PGCs during co-culture. These results suggested that cbMSCs from different ages can be used for co-culture H’Mong PGCs which were further used for genetic preservation of H’Mong chicken or gene editing research.

Keywords

Bone marrow mesenchymal stem cells
Different ages
Feeder layer
H’mong chicken
Primordial germ cells

Subjects

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Veterinary Research Forum
Volume 15, Issue 7
July 2024
Pages 335-342

  • Receive Date 19 November 2023
  • Revise Date 05 January 2024
  • Accept Date 07 April 2024

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