Veterinary Research Forum
Vet Res Forum

Molecular detection of the beta-lactamase Cefotaxime gene in Escherichia coli from the reproductive tract of Bali cattle with repeat breeder cases on Lombok island

Document Type : Original Article

Authors

Kholik Kholik 1
Pudji Srianto 2
Aulanni’am Aulanni’am 3
Sri Pantja Madyawati 2

1 Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Pendidikan Mandalika, Mataram, Indonesia

2 Department of Veterinary Reproduction, Faculty of Veterinary Medicine, Airlangga University, Surabaya, Indonesia

3 Laboratory of Department of Chemistry, Faculty of Veterinary Medicine, Universitas Brawijaya, Malang, Indonesia

https://doi.org/10.30466/vrf.2024.2014818.4056
Abstract
This study aims to detect Escherichia coli which encodes beta-lactamase Cefotaxime (blaCTX-M) gene from the reproductive tract of Bali cattle with repeat breeder cases. This research was conducted from June to August 2021 using 16 Bali cattle with repeat breeder cases. The reproductive fluids were taken using a plastic sheet gun which was inserted into a Brain Heart Infusion medium, isolated in eosin-methylene blue agar (EMB) and identified using biochemical tests. Antibiotic susceptibility testing of E. coli was carried out using the disc diffusion method. Double-disk approximation test was used to screen the presence of E. coli which produces Extended-spectrum beta-lactamase. The polymerase chain reaction (PCR) method was used to detect the blaCTX-M gene of E. coli and sequences of the blaCTX-M gene were phylogenetically analyzed. The research results obtained three E. coli isolates from 16 reproductive tract fluids of Bali cattle. Antibiotic sensitivity tests showed that 100% of E. coli was resistant to penicillin G and oxytetracycline. 66.66% of E. coli was resistant to cefotaxime (CTX) and gentamicin, and 33.33% of E. coli was resistant to tetracycline. Escherichia coli isolates that were resistant to penicillin and CTX showed positive results in the double-disk approximation test. The results of E. coli detection using PCR showed that three E. coli isolates encoded the blaCTX-M gene located at 370 bp on gel electrophoresis. The results of the phylogenetic analysis showed that E. coli from the reproductive tract of Bali cattle was related to E. coli that encoded blaCTX-M-14 isolated from humans.

Keywords

Bali cattle
blaCTX-M
Escherichia coli
Repeat breeder

Subjects

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Veterinary Research Forum
Volume 15, Issue 8
August 2024
Pages 397-402

  • Receive Date 01 November 2023
  • Revise Date 14 January 2024
  • Accept Date 04 March 2024

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