Veterinary Research Forum
Vet Res Forum

Investigating hydropericardium syndrome with different histopathological techniques in broiler chickens

Document Type : Original Article

Authors

Hossein Alavizadeh 1
Zahra Moosavi 1
Gholamreza Hashemi Tabar 2

1 Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

2 Department of Biotechnology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran

https://doi.org/10.30466/vrf.2024.2023088.4174
Abstract
Hydropericardium syndrome (HPS) has caused significant financial losses to the Iranian poultry industry in the past few years. Thirty-two broiler chickens with gross lesions of HPS were inspected histologically and immunohistochemically. Sampling was performed in Sabzevar, Iran. The dead and sick birds from random farms were subjected to necropsy examinations. Only four broiler chickens had no hydropericardium and the other gross findings were similar for birds. Basophilic and eosinophilic intranuclear inclusions, hemorrhages and necrosis in different organs were the primary characteristic histologic lesions. Lymphoid depletion, goblet cell hyperplasia and necrotizing enteritis were some of the findings reported in previous research. Low macrophage infiltration rate and brain lesions were other discoveries in Hematoxylin and Eosin (H&E) examination. Feulgen reaction and Cluster of Differentiation 68 (CD68) immunohistochemical staining were used for a comprehensive investigation and these techniques revealed improved histopathologic details. Feulgen staining confirmed brain lesions and some other changes in different organs. Eventually, the CD68 method revealed low macrophage presence in most organs. This study suggested that HPS might cause brain damage and the susceptibility of the Arian breed to the adenovirus needs further investigation.

Keywords

Feulgen staining
Histopathology
Hydropericardium syndrome
Immunohistochemistry
Iran

Subjects

  1. Asthana M, Chandra R, Kumar R. Hydropericardium syndrome: current state and future developments. Arch Virol 2013; 158(5): 921-931.
  2. Hess M. Detection and differentiation of avian adenoviruses: a review. Avian Pathol 2000; 29(3): 195-206.
  3. Asthana M, Singh VK, Kumar R, et al. Isolation, cloning and in silico study of hexon gene of fowl adenovirus 4 (FAV4) Isolates associated with hydro pericardium syndrome in domestic fowl. J Proteom Bioinform 2011; 4(9): 190-195.
  4. Abe T, Nakamura K, Tojo H, et al. Histology, immuno-histochemistry, and ultrastructure of hydro-pericardium syndrome in adult broiler breeders and broiler chicks. Avian Dis 1998; 42(3): 606-612.
  5. Ganesh K, Raghavan R. Hydropericardium hepatitis syndrome of broiler poultry: current status of research. Res Vet Sci 2000; 68(3): 201-206.
  6. Balamurugan V, Kataria JM. The hydropericardium syndrome in poultry-a current scenario. Vet Res Commun 2004; 28(2): 127-148.
  7. Nakamura K, Tanaka H, Mase M, et al. Pancreatic necrosis and ventricular erosion in adenovirus-associated hydropericardium syndrome of broilers. Vet Pathol 2002; 39(3): 403-406.
  8. Nakamura K, Mase M, Yamamoto Y, et al. Inclusion body hepatitis caused by fowl adenovirus in broiler chickens in Japan, 2009-2010. Avian Dis 2011; 55(4): 719-723.
  9. Shah MS, Ashraf A, Khan MI, et al. Fowl adenovirus: history, emergence, biology and development of a vaccine against hydropericardium syndrome. Arch Virol 2017; 162(7): 1833-1843.
  10. Asrani RK, Gupta VK, Sharma SK, et al. Hydro-pericardium-hepatopathy syndrome in Asian poultry. Vet Rec 1997; 141(11): 271-273.
  11. Kumar R, Chandra R, Shukla SK, et al. Hydro-pericardium syndrome (HPS) in India: a preliminary study on the causative agent and control of the disease by inactivated autogenous vaccine. Trop Anim Health Prod 1997; 29(3): 158-164.
  12. Niu Y, Sun Q, Zhu M, et al. Molecular epidemiology and phylogenetic analysis of fowl adenoviruses caused hydropericardium outbreak in China during 2015. Poult Sci 2018; 97(3): 803-811.
  13. Chandra R, Shukla SK, Kumar M. The hydroperi-cardium syndrome and inclusion body hepatitis in domestic fowl. Trop Anim Health Prod. 2000; 32(2): 99-111.
  14. Butterfield TA, Best TM, Merrick MA. The dual roles of neutrophils and macrophages in inflammation: a critical balance between tissue damage and repair. J Athl Train 2006; 41(4): 457-465.
Veterinary Research Forum
Volume 15, Issue 10
October 2024
Pages 559-564

  • Receive Date 18 February 2024
  • Revise Date 15 April 2024
  • Accept Date 07 May 2024

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