Veterinary Research Forum
Vet Res Forum

Effects of multi-enzymes supplementation to wheat and soybean meal-based feeds on growth performance, digestibility and carcass characteristics of quails

Document Type : Original Article

Authors

Mehmet Irmak 1
Muzaffer Denli 2
Veysi Kayri 3
İsa Coşkun 4

1 Department of Animal Nutrition and Nutritional Disease, Faculty of Veterinary Medicine, Siirt University, Siirt, Türkiye

2 Department of Animal Science, Faculty of Agriculture, Dicle University, Diyarbakır, Türkiye

3 Department of Medical Services and Techniques, Medical Laboratory Techniques Programme, Health Services Vocational School, Batman University, Batman, Türkiye

4 Department of Animal Science, Faculty of Agriculture, Ahi Evran University, Kırşehir, Türkiye

https://doi.org/10.30466/vrf.2024.2008986.3971
Abstract
Japanese quail (Coturnix coturnix japonica) is a popular experimental animal model in scientific research. The present study investigated the effects of dietary multiple enzyme supplementation on growth performance, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails fed diets based on wheat and soya bean meal. A total number of 192 1-day-old quails were assigned to three treatments with 16 replicates in each and four quails per replicate for 38 days.  The control group received a basal diet, and the treatment groups received a basal diet with 0.10 or 0.20% multi-enzyme, respectively. Growth performance parameters, carcass characteristics, nutrient digestibility and small intestinal histomorphology in quails were evaluated. Dietary supplementation of multi-enzymes to diet significantly increased body weight gain and improved the feed conversion rate. Moreover, quails fed with 0.10 or 0.20% multi-enzymes showed better ash digestibility coefficients and apparent metabolizable energy coefficients than the control quails. Furthermore, quails fed on a diet containing 0.20% multi-enzyme had the highest crude fiber digestibility. The villi length and the villi length/crypt depth ratio of the duodenum were significantly increased and the crypt depth was decreased in quails-fed diets supplemented with both multi-enzyme levels. However, feed consumption, carcass yield, carcass weight, heart weight, gizzard weight, liver weight and total intestine weights were not affected by treatments. In conclusion, our results showed that dietary supplementation of multi-enzymes to a wheat and soybean meal-based diet enhanced the growth performance and nutrient digestibility of quails.

Keywords

Growth performance
Intestinal histomorphology
Multi-enzymes
Nutrient digestibility
Quails

Subjects

  1. Al-Harthi MA. Impact of supplemental feed enzymes, condiments mixture or their combination on broiler performance, nutrients digestibility and plasma constituents. Int J Poult Sci 2006; 5(8): 764-771.
  2. Dipeolu MA, Eruvbetine D, Oguntona EB, et al. Com-parison of effects of antibiotics and enzyme inclusion in diets of laying birds. Arch Zootec 2005; 54: 3-11.
  3. Attia YA, Al-Khalaifah H, Abd El-Hamid HS, et al. Effect of different levels of multienzymes on immune response, blood hematology and biochemistry, anti oxidants status and organs histology of broiler chicks fed standard and low-density diets. Front Vet Sci 2020; 6: 510. doi: 10.3389/fvets.2019.00510.
  4. Suresh G, Santos DU, Rouissi T, et al. Production and in-vitro evaluation of an enzyme formulation as a potential alternative to feed antibiotics in poultry. Process Biochem 2019; 80: 9-16.
  5. Attia YA, Qota EMA, Aggoor FAM, et al. Value of rice bran, its maximal utilisation and its upgrading by phytase and other enzymes and diet-formulation based on available amino acids in the diet for broilers. Archiv Fur Geflugelkunde 2003; 67(4): 157-166.
  6. Cowieson AJ, Singh DN, Adeola O. Prediction of ingredient quality and the effect of a combination of xylanase, amylase, protease and phytase in the diets of broiler chicks. 1. Growth performance and digestible nutrient intake. Br Poult Sci 2006; 47(4): 477-489.
  7. Ojha BK, Singh PK, Shrivastava N. Enzymes in the animal feed In: Kuddus M (Ed). Enzymes in food biotechnology. 1st ed. Oxford, UK: Elsevier 2019; 93-109.
  8. Velázquez de Lucio BS, Hernández-Domínguez EM, Villa-Garcia M, et al. Exogenous enzymes as zoo-technical additives in animal feed: a review. Catalysts 2021; 11(7): 851. doi: 10.3390/catal11070851.
  9. Aftab U, Bedford MR. The use of NSP enzymes in poultry nutrition: myths and realities. Worlds Poult Sci J 2018; 74(2): 277-286.
  10. Choct M. Enzymes for the feed industry: past, present and future. Worlds Poult Sci J 2006; 62(1): 5-16.
  11. Bedford MR. Exogenous enzymes in monogastric nutrition - their current value and future benefits. Anim Feed Sci Technol 2000; 86(1-2): 1-13.
  12. Douglas MW, Parsons CM, Bedford MR. Effect of various soybean meal sources and Avizyme on chick growth performance and ileal digestible energy. J Appl Poult Res 2000; 9(1): 74-80.
  13. Jia W, Slominski BA, Guenter W, et al. The effect of enzyme supplementation on egg production parameters and omega-3 fatty acid deposition in laying hens fed flaxseed and canola seed. Poult Sci 2008; 87(10): 2005-2014.
  14. Yuan L, Wang M, Zhang X, et al. Effects of protease and non-starch polysaccharide enzyme on performance, digestive function, activity and gene expression of endogenous enzyme of broilers. PLoS One 2017; 12(3): e0173941. doi: 10.1371/journal.pone.0173941.
  15. Bedford MA. New enzyme technologies for poultry feeds. Br Poult Sci 2003; 44(Sup 1): 14-16.
  16. Irish GG, Balnave D. Non-starch polysaccharides and broiler performance on diets containing soyabean meal as the sole protein concentrate. Aust J Agric Res 1993; 44(7): 1483-1499.
  17. Al-Flayyih RNW, Kesab YGS. Effect of supplementation multi-enzyme on productive performance of two strains of Japanese quail. Int J Vet Sci Anim Husb 2023; 8(1) Part B: 123-132.
  18. Meng X, Slominski BA, Campbell LD, et al. The use of enzyme technology for improved energy utilization from full-fat oilseeds. Part I: canola seed. Poult Sci 2006; 85(6): 1025-1030.
  19. Slominski BA, Meng X, Campbell LD, et al. The use of enzyme technology for improved energy utilization from full-fat oilseeds. Part II: flaxseed. Poult Sci 2006; 85(6): 1031-1037.
  20. Kanbur G, Göçmen R, Cufadar Y. Effect of dietary banana leaves (Musa acuminata) with multienzyme complex supplementation on intestinal content, serum biochemicals, egg production, and egg quality in laying quail. Livest Sci 2023: 277: 105249. doi: 10.1016/ j.livsci.2023.105249.
  21. Mulaudzi A, Mnisi CM, Mlambo V. Effect of pre-treating dietary moringa oleifera leaf powder with fibrolytic enzymes on physiological and meat quality parameters in jumbo quail. Poultry 2022; 1: 54-65.
  22. Saikia AK, Bhuyan R, Saikia BN, et al. Effects of feeding graded levels of distillers dried grains with soluble (DDGS) with or without supplementation of multi-enzymes on blood bio-chemical constituents of indigenous chicken. IJAN 2019; 36(4): 382-387.
  23. Tüzün AE, Olgun O, Yıldız AÖ, et al. Effect of different dietary inclusion levels of sunflower meal and multi-enzyme supplementation on performance, meat yield, ileum histomorphology, and pancreatic enzyme activities in growing quails. Animals (Basel) 2020; 10(4): 680. doi: 10.3390/ani10040680.
  24. Gado SM, El-Gendi GM, Mohamady TF, et al. Effect of probiotic supplementation on productive performance of quail chicks. Ann Agric Sci Moshtohor 2013; 51(2): 107-116.
  25. Sebastian S, Touchburn SP, Chavez ER. Implications of phytic acid and supplemental microbial phytase in poultry nutrition: a review. Worlds Poult Sci J 1998; 54(1): 27-47.
  26. Nutrient requirements of poultry. 9th ed. Washington DC, USA: National Academy Press 1994; 44-45.
  27. Official method of Analysis. 18th ed. Association of Officiating Analytical Chemists. Washington DC, USA: 2005; Method 935.14 and 992.24.
  28. Marquardt RR. A simple spectrophotometric method for the direct determination of uric acid in avian excreta. Poult Sci 1983; 62(10): 2106-2108.
  29. Nkukwana TT, Muchenje V, Pieterse E, et al. Effect of Moringa oleifera leaf meal on growth performance, apparent digestibility, digestive organ size and carcass yield in broiler chickens. Livest Sci 2014; 161: 139-146.
  30. Adeola O. Digestion and balance techniques in pigs. In: Miller ER, Ullrey DE, Lewis AJ (Eds). Swine nutrition. 2nd Washington DC, USA: CRC Press 2001; 903-917.
  31. Aptekmann KP, Baraldi Artoni SM, Stefanini MA, et al. Morphometric analysis of the intestine of domestic quails (Coturnix coturnix japonica) treated with different levels of dietary calcium. Anat Histol Embryol 2001; 30(5): 277-280.
  32. Attia YA, Al-Khalaifah HS, Alqhtani AH, et al. The impact of multi-enzyme fortification on growth performance, intestinal morphology, nutrient digestibility, and meat quality of broiler chickens fed a standard or low-density diet. Front Vet Sci 2022; 9: 1012462. doi: 10.3389/fvets.2022.1012462.
  33. Chimote MJ, Barmase BS, Raut AS, et al. Effect of supplementation of probiotic and enzymes on performance of Japanese quails. Vet World  2009; 2(6): 219-220.
  34. Rabie MH, El Maaty HA. Growth performance of Japanese quail as affected by dietary protein level and enzyme supplementation. Asian J Anim Vet Adv 2015; 10(2): 74-85.
  35. Mushtaq T, Sarwar M, Ahmad G, et al. Influence of canola meal-based diets supplemented with exogenous enzyme and digestible lysine on performance, digestibility, carcass, and immunity responses of broiler chickens. Poult Sci 2007; 86(10): 2144-2151.
  36. Horvatovic MP, Glamocic D, Zikic D, et al. Performance and some intestinal functions of broilers fed diets with different inclusion levels of sunflower meal and supplemented or not with enzymes. Braz J Poult Sci 2015; 17(1): 25-30.
  37. Alagawany M, Attia AI, Ibrahim ZA, et al. The effectiveness of dietary sunflower meal and exogenous enzyme on growth, digestive enzymes, carcass traits, and blood chemistry of broilers. Environ Sci Pollut Res Int 2017; 24(13): 12319-12327.
  38. Feng Y, Wang L, Khan A, et al. Fermented wheat bran by xylanase-producing Bacillus cereus boosts the intestinal microflora of broiler chickens. Poult Sci 2020; 99(1): 263-271.
  39. Kies AK, Van Hemert KHF, Sauer WC. Effect of phytase on protein and amino acid digestibility and energy utilisation. Worlds Poult Sci J 2001; 57(2): 109-126.
  40. Ravindran V, Selle PH, Bryden WL. Effects of phytase supplementation, individually and in combination, with glycanase, on the nutritive value of wheat and barley. Poult Sci 1999; 78(11): 1588-1595.
  41. Geyra A, Uni Z, Sklan D. The effect of fasting at different ages on growth and tissue dynamics in the small intestine of the young chick. Br J Nutr 2001; 86(1): 53-61.
  42. de Oliveira-Bruxel TM, Nunes RV, Eyng C, et al. Sunflower meal and exogenous enzymes in initial diets for broilers. Rev Caatinga 2016; 29(4): 996-1005.
  43. Farahat M, Ibrahim D, Kishawy ATY, et al. Effect of cereal type and plant extract addition on the growth performance, intestinal morphology, caecal microflora, and gut barriers gene expression of broiler chickens. Animal 2021; 15(3): 100056. doi: 10.1016/j.animal. 2020.100056.
Veterinary Research Forum
Volume 15, Issue 11
November 2024
Pages 583-590

  • Receive Date 10 August 2023
  • Revise Date 04 March 2024
  • Accept Date 05 June 2024

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