Veterinary Research Forum
Vet Res Forum

An immunohistochemical study on the evaluation of mast cell, interleukin 17 and interleukin 1β profile in contagious caprine pleuropneumonia

Document Type : Original Article

Authors

Ozhan Karatas 1
Gokhan Akcakavak 2

1 Department of Pathology, Faculty of Veterinary Medicine, Sivas Cumhuriyet University, Sivas, Türkiye

2 Department of Pathology, Faculty of Veterinary Medicine, Aksaray University, Aksaray, Türkiye

https://doi.org/10.30466/vrf.2024.2023580.4184
Abstract
Contagious caprine pleuropneumonia (CCPP) in goats is defined as a highly contagious and rapidly spreading mycoplasmal disease that is now among the leading causes of major economic losses on many continents (Asia, Africa and the Middle East). In this study, we aimed to evaluate immunohistochemically mast cells (MCs) profile and local interleukin (IL)-17 and IL-1β protein expressions in naturally infected CCPP according to the course of the inflammation (peracute-acute, subacute-chronic). The material of the study consisted of 40 naturally infected CCPP and 6 healthy control goat lung tissues. Appropriate samples were taken from the necropsied goats and subjected to histopathological and immunohistochemical examination. In the histopathological examination of the samples, it was determined that 29 samples had a peracute-acute course and 11 had a subacute-chronic course. In immuno-histochemical examination, MC profile and local IL-17 and IL-1β protein expressions were evaluated in the peracute-acute and subacute-chronic course. Immunohistochemically, significant increases in MC number, local IL-17 and IL-1β scores were detected in the peracute-acute course compared to the control group. There were significant decreases in the relevant scores in the subacute-chronic course compared to the peracute-acute course. Current findings indicated that MC, IL-17, and IL-1β expressions played important roles in the pathogenesis of infection in naturally infected CCPP, especially in the peracute-acute course. Additionally, MC profile was evaluated for the first time in naturally infected CCPP.

Keywords

Caprine
Interleukin-1β
Interleukin-17
Immunohistochemistry
Mast cell

Subjects

  1. Srivastava AK, Meenowa D, Barden G, et al. Contagious caprine pleuropneumonia in Mauritius. Vet Rec 2010; 167(8): 304-305.
  2. Tigga M, Choudhary BK, Ghosh RC, et al. Mycoplasmosis: an emerging threat to developing livestock industry. Int J Adv Res 2014; 2(1): 558-564.
  3. Khodakaram-Tafti A, Derakhshandeh A, A Daee A, et al. Identification of Mycoplasma capricolum subspecies capripneumoniae and Mycoplasma arginini by culture, PCR, and histopathology in pneumonic lungs of slaughtered goats in Mashhad, Iran. Iran J Vet Res 2023; 24(2): 96-101.
  4. Radostits OM, Gay C, Hinchcliff KW, et al. A textbook of the diseases of cattle, horses, sheep, pigs and goats. 10th London, UK: Saunders 2007; 2045-2050.
  5. Iqbal Yatoo M, Raffiq Parray O, Tauseef Bashir S, et al. Contagious caprine pleuropneumonia - a comprehensive review. Vet Q 2019; 39(1): 1-25.
  6. Çiftçi M, Ortatatlı M, Erer H, Hatipoğlu F, Özdemir Ö. Veterinary systemic pathology 1 [Turkish]. 4th Konya, Türkiye: Nobel Bookstore 2020; 122-132.
  7. Thiaucourt F, Bölske G, Leneguersh B, et al. Diagnosis and control of contagious caprine pleuropneumonia. Rev Sci Tech 1996; 15(4): 1415-1429.
  8. Abd-Elrahman AH, Khafaga AF, Abas OM. The first identification of contagious caprine pleuropneumonia (CCPP) in sheep and goats in Egypt: molecular and pathological characterization. Trop Anim Health Prod 2020; 52(3): 1179-1186.
  9. Mondal D, Pramanik A, Basak DK. Clinico-haematology and pathology of caprine mycoplasmal pneumonia in rain fed tropics of West Bengal. Small Rumin Res 2004; 51(3): 285-295.
  10. Johnzon CF, Rönnberg E, Pejler G. The role of mast cells in bacterial infection. Am J Pathol 2016; 186(1): 4-14.
  11. Wesolowski J, Paumet F. The impact of bacterial infection on mast cell degranulation. Immunol Res 2011; 51(2-3): 215-226.
  12. Chacón-Salinas R, Chen L, Chávez-Blanco AD, et al. An essential role for platelet-activating factor in activating mast cell migration following ultraviolet irradiation. J Leukoc Biol 2014; 95(1): 139-148.
  13. St John AL, Abraham SN. Innate immunity and its regulation by mast cells. J Immunol 2013; 190(9): 4458-4463.
  14. Boyce JA. Eicosanoid mediators of mast cells: receptors, regulation of synthesis, and pathobiologic implications. Chem Immunol Allergy 2005; 87: 59-79.
  15. Mukai K, Tsai M, Saito H, et al. Mast cells as sources of cytokines, chemokines, and growth factors. Immunolo Rev 2018; 282(1): 121-150.
  16. McGeachy MJ, Cua DJ, Gaffen SL. The IL-17 family of cytokines in health and disease. Immunity 2019; 50(4): 892-906.
  17. Puerta-Arias JD, Mejía SP, González Á. The role of the interleukin-17 axis and neutrophils in the pathogenesis of endemic and systemic mycoses. Front Cell Infect Microbiol 2020; 10: 595301. doi: 10.3389/ fcimb.2020.595301.
  18. De Filippo K, Dudeck A, Hasenberg M, et al. Mast cell and macrophage chemokines CXCL1/CXCL2 control the early stage of neutrophil recruitment during tissue inflammation. Blood 2013; 121(24): 4930-4937.
  19. Wang P, Qian H, Xiao M, et al. Role of signal transduction pathways in IL‐1β‐induced apoptosis: pathological and therapeutic aspects. Immun Inflamm Dis 2023; 11(1): e762. doi: 10.1002/iid3.762.
  20. Akcakavak G, Kazak F, Deveci MZY. Eucalyptol protects against cisplatin-induced liver injury in rats. Biol Bull 2023; 50(5): 987-994.
  21. Sener S, Ipek V. Investigation of brain mast cells in ovine encephalitic listeriosis. Biotech Histochem 2022; 97(4): 247-253.
  22. Smith BP, VanMetre D, Pusterla N. Large animal internal medicine-E-Book. 6th St. Louis, USA: Elsevier 2019; 1949.
  23. Rivera J, Fierro NA, Olivera A, et al. New insights on mast cell activation via the high affinity receptor for IgE. Adv Immunol 2008; 98: 85-120.
  24. Gekara NO, Weiss S. Mast cells initiate early anti‐Listeria host defences. Cell Microbiol 2008; 10(1): 225-236.
  25. Xu X, Zhang D, Lyubynska N, et al. Mast cells protect mice from Mycoplasma pneumonia. Am J Respir Crit Care Med 2006; 173(2): 219-225.
  26. Sutherland RE, Olsen JS, McKinstry A, et al. Mast cell IL-6 improves survival from Klebsiella pneumonia and sepsis by enhancing neutrophil killing. J Immunol 2008; 181(8): 5598-5605.
  27. Luo Y, Li C, Zhou Z, et al. Biological functions of IL‐17‐producing cells in mycoplasma respiratory infection. Immunology 2021; 164(2): 223-230.
  28. Wu Q, Martin RJ, Rino JG, et al. IL-23-dependent IL-17 production is essential in neutrophil recruitment and activity in mouse lung defense against respiratory Mycoplasma pneumoniae infection. Microbes Infect 2007; 9(1): 78-86. 
  29. Ma WT, Gu K, Yang R, et al. Interleukin-17 mediates lung injury by promoting neutrophil accumulation during the development of contagious caprine pleuropneumonia. Vet Microbiol 2020; 243: 108651. doi: 10.1016/j.vetmic.2020.108651. 
  30. Herrero-Cervera A, Soehnlein O, Kenne E. Neutrophils in chronic inflammatory diseases. Cell Mol Immunol 2022; 19(2): 177-191.
  31. Reed M, Morris SH, Owczarczyk AB, et al. Deficiency of autophagy protein Map1-LC3b mediates IL-17-dependent lung pathology during respiratory viral infection via ER stress-associated IL-1. Mucosal Immunol 2015; 8(5): 1118-1130.
  32. Kolaczkowska E, Kubes P. Neutrophil recruitment and function in health and inflammation. Nat Rev Immunol 2013; 13(3): 159-175.
  33. Abraham SN, St. John AL. Mast cell-orchestrated immunity to pathogens. Nat Rev Immunol 2010; 10(6): 440-452.
Veterinary Research Forum
Volume 15, Issue 10
October 2024
Pages 515-521

  • Receive Date 22 February 2024
  • Revise Date 14 March 2024
  • Accept Date 07 April 2024

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